Age-0 yellow perch density (#/ha) Year Month Preservation 18 mm Trawl Seine Pelagic End Member Mean (SD) Taxonomic Groups* Benthic End Member Mean (SD) Taxonomic Groups* Individual-level Population-level Offshore Inshore % Benthic % Benthic % Benthic % Benthic (a in = 0.25) (a in = 0.43) 1965 July Formaldehyde 154110 26662 8231 -31.99 (0.86) b,d,e,f,i,m -27.46 (1.55) a,d 0.2 81.3 7.8 15.5 1965 August Formaldehyde 12392 1652 -29.68 (0.97) a,e,f,m -24.49 (0.33) a,d,i 0.6 39.9 2.3 4.2 1966 July Formaldehyde 44220 25725 4693 -32.29 (0.48) b,d,e,f -25.17 (1.58) a,d,h 17.0 46.4 18.7 20.6 1966 August Formaldehyde 4602 1639 -31.60 (0.29) a,b,d,e,f -24.86 (0.67) a,d,l,m 2.4 42.9 6.7 11.0 1967 July Formaldehyde 67320 40025 5970 -30.16 (0.63) a,d,e,f,m -25.23 (3.32) a,h,l 7.1 2.7 6.9 6.6 1967 August Formaldehyde 14457 4326 -30.37 (0.83) a,d,e,f -24.59 (-) a 7.5 37.3 10.2 13.0 1970 July Formaldehyde 88000 25066 14313 -30.40 (0.99) a,d,e,f,h -24.41 (1.32) a,d,h,l -2.8 43.8 4.7 11.2 1970 August Formaldehyde 7369 4809 -30.42 (0.64) a,e,f -25.40 (1.08) a,d,e,g,h 10.0 55.5 18.1 25.0 2007 July Frozen 138760 13368 9748 -32.83 (1.71) a,d,e,f -23.34 (1.52) a,d,l 13.4 66.8 23.8 32.3 2007 August Frozen 6805 6853 -32.20 (1.03) a,e,f,j -23.29 (0.91) a,d,e,h,i,k,l 18.1 93.8 37.1 50.8 2008 July Frozen 67680 2007 20923 -32.30 (0.81) e,f -24.93 (1.00) a,d,h,i,l,m 8.4 80.0 64.0 71.9 2008 August Frozen 634 4501 -31.02 (0.44) d,e,f -23.05 (1.24) a,c,d,e,h,i,l,m 7.9 78.9 57.8 67.8 2009 July Frozen 125621 5257 8896 -32.64 (0.67) a,e,f -23.05 (1.00) a,d,f,i 4.2 77.0 30.4 45.0 2009 August Frozen 3126 6405 -30.28 (0.58) a,e,f,l -22.51 (0.81) a,d,f,k,l -2.6 78.2 30.2 46.5 2011 July Frozen 4713 519 2784 -32.70 (1.11) a,e -25.32 (1.16) a,d 7.1 76.9 51.9 63.1 2011 August Frozen 164 1115 -30.25 (0.79) a,d,e,m -23.06 (1.53) a,d,m 7.5 78.2 56.6 66.7 * - Taxonomic groups from diets included in pelagic and benthic end member calculations are indicated with the following letters: a - amphipod, b - bosmina, c - chaoborus, d - chironomid larvae, e - cladoceran, f - copepod, g - corixidae, h - ephemeroptera, i - isopod, j - leptodora, k - chironomid pupae, l - tricopterans, m - zooplankton. Table S1. Summary Table of Samples and Analyses by Month/Year Month/year sampled, preservation technique, age-0 yellow perch density (18 mm, trawl, seine), mean pelagic and benthic end member values (mean ± 1 SD) and taxonomic groups used in calculations, individual-level percent benthic reliance for demersal and littoral age-0 yellow perch, and population level percent benthic reliance of age-0 yellow perch at in = 0.25 and 0.43. Taxonomic Groups Year Month n Length (mm) Cladocerans Copepods Amphipods Chironomids Trichoptera Isopod Zooplankton Other* Offshore 1965 July 18 36.7 (2.4) 25.6 6.9 0.1 0.2 0.0 0.3 12.0 0.0 1965 August 20 48 (4.9) 29.7 7.5 0.3 0.0 0.0 0.0 287.3 0.0 1966 July 21 36.3 (4.4) 26.5 0.8 0.0 0.0 0.0 0.0 0.0 0.1 1966 August 19 57.4 (4.8) 19.2 14.6 0.3 0.2 0.0 0.0 1.9 0.8 1967 July 20 41.0 (2.5) 33.0 39.1 0.2 0.4 0.0 0.0 0.5 0.0 1967 August 15 55.5 (5.0) 60.2 12.9 0.2 0.3 0.0 0.0 3.3 0.0 1970 July 18 43.0 (5.2) 63.9 0.5 0.6 0.1 0.0 0.0 0.0 0.1 1970 August 20 55.3 (6.3) 20.2 4.1 1.8 0.1 0.0 0.0 0.0 0.0 2007 July 20 48.0 (5.5) 48.1 41.1 0.4 1.4 0.0 0.0 0.0 16.0 2007 August 32 62.9 (8.8) 391.4 43.3 0.3 0.1 0.0 0.0 0.8 0.0 2008 July 19 51.6 (4.0) 35.0 69.4 0.0 0.2 0.0 0.0 0.2 0.0 2008 August 26 60.3 (6.0) 8.8 69.4 0.1 0.2 0.0 0.0 0.4 0.0 2009 July 21 48.8 (3.0) 27.7 19.8 0.0 0.0 0.0 0.0 0.2 0.0 2009 August 21 65.2 (5.9) 73.7 14.4 6.3 0.0 0.1 0.0 0.2 0.0 2011 July 20 55.6 (4.4) 82.9 5.4 0.2 0.0 0.0 0.0 0.3 0.1 2011 August 20 62.7 (5.5) 9.0 2.7 0.8 10.4 0.1 0.1 21.6 0.2 Inshore 1965 July 20 41.3 (5.5) 0.0 0.0 1.2 2.9 0.0 0.0 8.5 0.0 1965 August 20 49.1 (6.6) 0.0 0.4 11.3 1.1 0.0 0.1 50.0 0.0 1966 July 22 44.0 (5.7) 23.9 1.6 2.5 0.3 0.0 0.0 81.1 0.1 1966 August 20 54.6 (6.5) 37.8 0.0 2.6 0.2 0.1 0.0 23.3 0.1 1967 July 18 36.6 (1.4) 0.8 0.8 2.2 0.6 0.2 0.0 0.0 0.2 1967 August 22 48.6 (4.5) 26.9 0.0 1.7 0.6 0.0 0.0 0.0 0.0 1970 July 20 46.2 (6.4) 64.3 0.1 3.3 2.8 0.1 0.0 0.0 0.2 1970 August 20 58.6 (5.8) 9.3 0.0 3.5 1.7 0.0 0.0 18.1 0.1 2007 July 20 50.0 (6.4) 1.4 2.4 2.6 0.8 2.0 0.0 0.4 0.4 2007 August 25 62.2 (8.0) 11.4 11.4 9.0 1.5 0.2 0.1 0.0 0.1 2008 July 20 52.8 (6.1) 0.2 0.2 4.0 5.6 0.3 0.1 0.0 0.0 2008 August 28 66.6 (10.3) 0.0 1.4 5.5 1.7 1.9 0.0 3.1 0.3 2009 July 21 50.4 (4.9) 7.2 23.2 2.6 0.9 0.4 0.1 14.2 2.0 2009 August 20 66.3 (8.4) 0.9 5.3 5.9 3.0 0.2 0.0 0.3 0.0 2011 July 20 54.9 (3.8) 2.6 9.0 3.4 5.0 0.6 0.0 0.2 0.9 2011 August 20 68.7 (6.2) 9.5 5.7 5.5 4.1 0.5 0.0 0.3 0.3 * - Taxonomic groups represented in other category include: clams, corixidae, eggs, ephemeroptera, hemiptera ,mites, and snails. Table S2. Summary of Diet Composition for Demersal and Littoral Age-0 Yellow Perch Sample size, mean length (± 1 SD), and mean number of items observed in diets of age-0 yellow perch for each month/year combination. Only the dominant taxonomic groups observed are included (represent >99% of all diet items).