Making Solutions Review Sheet No. 1 Convert the values as indicated. Specify the appropriate instrument with which the final measurements should be made. For items 10-13, show the calculation (equation and units) for the preparation of each solution. Instrument Choices graduated cylinder 2-ml pipet P1000 analytical balance 10-ml pipet 1-ml pipet P100 1. 3.4 ml = ________ µl 4. 73.12 µg = _______ mg 7. 10.5 µl = ________ ml ____________ ____________ ____________ 2. 43.0 ml = ________ L 5. 5.39 g = ________ mg 8. 7.503 ml = ________ µl ____________ ____________ ____________ 3. 0.17 ml = ________ µl 6. 30.6 g = __________ mg 9. 33 µg = ________ mg ____________ ____________ ____________ Solutions to Be Prepared 10. Prepare 25 ml of 2.5 g/ml NaCl solution 5-ml pipet table top balance P10 Calculations 11. Prepare 10 ml of 50 mg/ml CuSO4 solution 12. Prepare 2 L of 0.5 g/ml dextrose solution 13. Prepare 100 ml of 0.005 g/ml NaOH solution Taken from Biotechnology: Science for the New Millennium Laboratory Manual by Ellyn Daugherty, page 49 Making Solutions Review Sheet No. 2 Convert the values as indicated. Specify the appropriate instrument with which the final measurements should be made. For items 10-13, show the calculation (equation and units) for the preparation of each solution. Instrument Choices graduated cylinder 2-ml pipet P1000 analytical balance 10-ml pipet 1-ml pipet P100 1. 0.079 L = _______ ml 4. 9.22 mg = _______ g ____________ 2. 10.72 µl = ______ml ____________ 3. 0.3 ml = ________ µl ____________ 5-ml pipet table top balance P10 7. 0.085 ml = ________ µl ____________ ____________ 5. 841 µg = ________ mg 8. 1.223 ml = _______ µl ____________ ____________ 6. 3.64 g = _________ mg ____________ Solutions to Be Prepared 10. Prepare 40 ml of 6.5 mg/ml CuSO4 pentahydrate solution 9. 0.19 g = ________ µg ____________ Calculations 11. Prepare 20 ml of 8% NaCl solution 12. Prepare 0.75 L of 5% dextrose solution 13. Prepare 10 ml of 1.25% NaOH solution Taken from Biotechnology: Science for the New Millennium Laboratory Manual by Ellyn Daugherty, page 54 Making Solutions Review Sheet No. 3 Convert the values as indicated. Specify the appropriate instrument with which the final measurements should be made. For items 7-11, show the calculation (equation and units) for the preparation of each solution. Instrument Choices graduated cylinder 2-ml pipet P1000 analytical balance 10-ml pipet 1-ml pipet P100 1. 0.42 g = ______ µg 3. 90.22 µg = _________ g 5. 0.0285 ml = ______ µl ____________ ____________ ____________ 2. 999 µl = ________ ml 4. 80.41 ml = ________ µl 5. 70.503 mg = ________ g ____________ ____________ ____________ Solutions to Be Prepared 7. Prepare 550 ml of 9.5 mg/ml NaOH solution 5-ml pipet table top balance P10 Calculations 8. Prepare 150 ml of 2% CuSO4 .5H2O solution 9. Prepare 3 L of 0.025% dextrose solution 10. Prepare 125 ml of 10 M NaOH solution 11. Prepare 75 ml of 0.1 M NaCl solution Taken from Biotechnology: Science for the New Millennium Laboratory Manual by Ellyn Daugherty, page 58 Making Solutions Review Sheet No. 4 Show the calculation (equation and units) for the preparation of each solution. Solutions to Be Prepared 1. Prepare 100ml of 15 mg/ml NaOH from 100 mg/ml NaOH Calculations 2. Prepare 10 ml of 0.5 CuSO4.5H2O solution from 10 M CuSO4.5H2O 3. Prepare 2 L of 5 mg/ml gelatin solution from 1 g/ml gelatin 4. Prepare 950 ml of 1 X CuSO4.5H2O solution from 25 X CuSO4.5H2O 5. Prepare 5 L of 0.2 M dextrose solution from 5 M dextrose solution 6. Prepare 100 ml of 2.5 X NaOH from 50X NaOH 7. Prepare 50 ml of 5 mM NaCl from 1 M NaCl Taken from Biotechnology: Science for the New Millennium Laboratory Manual by Ellyn Daugherty, page 62