LOCUS
2013
DEFINITION
Exported File
2788 bp ds-DNA
circular SYN 18-APR-
Entry vector for transferring a cloned attL-flanked gene to a
destination vector in the Gateway(R) system.
ACCESSION
.
VERSION
.
KEYWORDS
pENTR223 with ORF
SOURCE
synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE
1 (bases 1 to 2788)
AUTHORS
I.M.A.G.E Consortium
TITLE
Direct Submission
JOURNAL
Exported Thursday, Apr 18, 2013 from SnapGene 1.5.2
http://www.snapgene.com
FEATURES
Location/Qualifiers
source
1..2788
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
terminator
268..295
/note="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator
primer_bind
protein_bind
/note="color: #ffffff"
387..473
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
/note="color: #ffffff"
537..553
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
/note="color: #a020f0; direction: RIGHT"
569..662
/bound_moiety="LR Clonase(TM)"
/note="attL1'"
/note="recombination site for the Gateway(R) LR
reaction;
gap
misc_feature
protein_bind
mutated version of attL1"
/note="color: #31849b"
663..672
/estimated_length="10"
/note="color: #------"
663..672
/note="ORF insertion site"
/note="color: #a6acb3"
673..766
/bound_moiety="LR Clonase(TM)"
/note="attL2'"
/note="recombination site for the Gateway(R) LR
reaction;
promoter
primer_bind
CDS
mutated version of attL2"
/note="color: #31849b"
complement(784..802)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
/note="color: #ffffff; direction: LEFT"
complement(807..823)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
/note="color: #a020f0; direction: LEFT"
1254..2045
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase"
/note="SmR"
/note="confers resistance to spectinomycin and
streptomycin"
/note="color: #ccffcc"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
rep_origin
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
2138..2726
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
ORIGIN
1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
//
ctttcctgcg
taccgctcgc
gcgcccaata
cgacaggttt
tagccaggaa
gtttgatgcc
acaacgttca
caacagataa
gcagttccct
aacgacggcc
ctgttcgttg
agnnnnnnnn
gttgcaacga
tcccctatag
ctcaaaatct
ccagccagga
cgtggaaacg
aatgcaagta
gtaacggcgc
tgcctcgggc
gcagcaacga
aagcggtgat
atctcgaacc
agccacacag
ggcgagcttt
tccgcgctgt
ctaagcgcga
agccagccac
ttgccttggt
ttgaggcgct
agcgaaatgt
cgccgaagga
tcatacttga
cagatcagtt
aataaccctc
gagcgtcaga
taatctgctg
aagagctacc
ctgtccttct
catacctcgc
ttaccgggtt
ggggttcgtg
agcgtgagca
taagcggcag
atctttatag
cgtcaggggg
ccttttgctg
ttatcccctg
cgcagccgaa
cgcaaaccgc
cccgactgga
gagtttgtag
tggcagttta
aatccgctcc
aacgaaaggc
actctcgcgt
agtcttaagc
caacaaattg
nnctttcttg
acaggtcact
tgagtcgtat
ctgatgttac
cagaaatgcc
gatgaaggca
gcgtatgcgc
agtggcggtt
atccaagcag
tgttacgcag
cgccgaagta
gacgttgctg
tgatattgat
gatcaacgac
agaagtcacc
actgcaattt
gatcgacatt
aggtccagcg
aaatgaaacc
agtgcttacg
tgtcgctgcc
agctagacag
ggaagaattt
gagccaccca
ccccgtagaa
cttgcaaaca
aactcttttt
agtgtagccg
tctgctaatc
ggactcaaga
cacacagccc
ttgagaaagc
ggtcggaaca
tcctgtcggg
gcggagccta
gccttttgct
attctgtgga
cgaccgagcg
ctctccccgc
aagcgggcag
aaacgcaaaa
tggcgggcgt
cggcggattt
ccagtcttcc
taacgctagc
tcgggcccca
atgagcaatg
tacaaagttg
atcagtcaaa
tacatggtca
attgcacaag
tcgacttcgc
cgaacccagt
tcacgcaact
ttcatggctt
caagcgcgtt
cagggcagtc
tcgactcaac
gccgtacatt
ttgctggtta
cttttggaaa
attgttgtgc
ggagaatggc
gatctggcta
gcggaggaac
ttaacgctat
ttgtcccgca
gactgggcaa
gcttatcttg
gtccactacg
tgaccaaaat
aagatcaaag
aaaaaaccac
ccgaaggtaa
tagttaggcc
ctgttaccag
cgatagttac
agcttggagc
gccacgcttc
ggagagcgca
tttcgccacc
tggaaaaacg
cacatgtt
taaccgtatt
cagcgagtca
gcgttggccg
tgagcgcaac
aggccatccg
cctgcccgcc
gtcctactca
gactgagcct
atggatgttt
aataatgatt
cttttttata
gcattataag
ataaaatcat
tagctgtttc
ataaaaatat
tgctacccaa
ggacataagc
ggtccagaac
gttatgactg
acgccgtggg
gccctaaaac
tatcagaggt
tgtacggctc
cggtgaccgt
cttcggcttc
acgacgacat
agcgcaatga
tcttgctgac
tctttgatcc
ggaactcgcc
tttggtacag
tggagcgcct
gacaagaaga
tgaaaggcga
cccttaacgt
gatcttcttg
cgctaccagc
ctggcttcag
accacttcaa
tggctgctgc
cggataaggc
gaacgaccta
ccgaagggag
cgagggagct
tctgacttga
ccagcaacgc
accgcctttg
gtgagcgagg
attcattaat
gcaattaata
tcaggatggc
accctccggg
ggagagcgtt
ttcgttttat
tcccagtcac
ttattttgac
atgccaactt
aaagcattgc
tatttgccat
ctggcagctc
atcatcatgc
ggttgccggg
ctgttcggtt
cttgaccgaa
tttttttggg
tcgatgtttg
aaagttaaac
agttggcgtc
cgcagtggat
aaggcttgat
ccctggagag
cattccgtgg
cattcttgca
aaaagcaaga
ggttcctgaa
gcccgactgg
cgcagtaacc
gccggcccag
agatcgcttg
gatcaccaag
gagttacgcg
agatcctttt
ggtggtttgt
cagagcgcag
gaactctgta
cagtggcgat
gcagcggtcg
caccgaactg
aaaggcggac
tccaggggga
gcgtcgattt
ggccttttta
agtgagctga
aagcggaaga
gcagctggca
cgcgtaccgc
cttctgctta
ccgttgcttc
caccgacaaa
ttgatgcctg
gacgttgtaa
tgatagtgac
tgtacaaaaa
ttatcaattt
ccagctgata
tggcccgtgt
ctcctctaga
tgacgcacac
cgtaagctgt
cgcagcggtg
gtacagtcta
atgttatgga
atcatgaggg
atcgagcgcc
ggcggcctga
gaaacaacgc
agcgagattc
cgttatccag
ggtatcttcg
gaacatagcg
caggatctat
gctggcgatg
ggcaaaatcg
tatcagcccg
gcctcgcgcg
gtagtcggca
tcgttccact
tttctgcgcg
ttgccggatc
ataccaaata
gcaccgccta
aagtcgtgtc
ggctgaacgg
agatacctac
aggtatccgg
aacgcctggt
ttgtgatgct
cggttcctgg