ELECTRONIC SUPPLEMENTARY MATERIAL
Fine mapping of the dull fruit skin gene D in cucumber (Cucumis
sativus L.)
MOLECULAR BREEDING
Xu-Qin Yang1, Wei-wei Zhang2, Yue Li1, Huan-Le He1, Bei-Bei Bie1, Guo-Liang Ren1, Jun-Long
Zhao1, Yun-Li Wang1, Jing-Tao Nie1, Jun-Song Pan1*, Run Cai1*

1 School of Agriculture and Biology, Shanghai Jiao Tong University, Dongchuan Road, Minhang District, Shanghai
200240, China
2  Shanghai Chenshan Plant Science Research Center, Chinese Academy of Sciences, Chenshan Botanical Garden,
Shanghai 201602, China
* Corresponding authors. J.S. Pan, E-mail: jspan71@sjtu.edu.cn; R. Cai, E-mail: cairun@sjtu.edu.cn
Supplementary Table S1 The primers used to construct the genetic map of the dull fruit skin gene D
Markers
Forward primer sequences(5’-3’)
Reverse primer sequences(5’-3’)
Polymorphism
SSR04812
AGATATGTAGACCATCCAAAGCA
TCTTTTCATACGTGGGTCCTG
Co-dominant
SSR07100
CACACCATTTACGGTTATGGG
CATTTGGTTCAGAAAGGGGA
Co-dominant
SSR03529
TGAATTGAATAGACACAACAATATGC
ACATGTTGGGACTCCATGTG
Co-dominant
SSR16068
TATAACCCCGTCTTCTCCCC
GGGAAACCCCTTTGACTCTC
Co-dominant
SCZ24
CCGGAGCTGTGTAATGGAAGAA
AATTTGAGATATAACCTACGTGA
Co-dominant
SCZ69
TTCCGAAAGCAGGAGAGTCAAT
GCCAGATTTGGTATATCAAACAG
Dominant
SSR16203
TCGAGGTAAATCAAAACCGA
ATGTGTCAAACCCACCCATT
Co-dominant
SSR04142
GCAAAAGTAGAAAAGCGCGA
TGTTGTCATTACCAAATTGATTCT
Co-dominant
SSR15818
GGACATGTCAACTCCCCTGT
GCCTCTAGCCTGAAAGACCA
Co-dominant
SSR15321
TCAATGTAGGTAGAGCACCACG
TCCAATTGCTTGACCAATGA
Co-dominant
SSR04644
CCAAAAGCTTTCTCAAATAATGG
CTCTGCTCTCCATTTACGCC
Co-dominant
Note: These 11 markers were reported in Zhang et al. (2012).
Supplementary Table S2 The primers used for semi-quantitative RT-PCR ananlysis of 31 candidate genes
Genes ID
Forward primer sequences(5’-3’)
Reverse primer sequences(5’-3’)
Csa016877
TCAGTCGTCGTTCCAGTTT
CCCATAGCGGATTTCAGT
Csa016878
AAGAAAGTCAGAAGTGAAGGTG
AAGTGGTATGCTCCCAAAGA
Csa016879
CGTTCTCATTTTAGCATTTG
CCATAGAACCACAGCGACT
Csa016880
ATTGAACCAAAGGCTACTATGT
GCAACAACCCTCATTTTCTC
Csa016881
TACGGAGGAAGGGTAGAGAT
CTGGATAACACTGCCAATAA
Csa016882
TCTTTCTAACCTCAAAACCAA
CAGTTCCCATCTCCATCTT
Csa016883
TACCAAACTTTCGCTCCTG
CTAAATGGAAAGGGTGGC
Csa016884
CAGTGCTCTTCTTAGTGGGTAT
TTGGAATGATGCTGCTTT
Csa016885
ATGGCACTTTGCTCTTCTG
AACCTTGCCAGATTCAGC
Csa016886
CTCGAAGCAAATCACATAAG
TTTCTCTTCAATCAACAGCA
Csa016887
TACTGGATAAACTGAGGAGATA
GCTTTGTAACTTGGCTTCC
Csa016888
TTGTTGGGACATTGGGATA
ATGGGAGTCTGCTATTCTTG
Csa016889
TTAGTGGTGGCTTTACGAA
GCACCAAAAGCAGAGAAA
Csa016890
TCTCCTACGCTACAGTTTCAC
TCTTCTTCAAATCTTCTCGTT
Csa016891
ATCCACAGAGGGAGGTCA
TATTGACACGACCCAAGTTAT
Csa016892
TGGTGTGATGACCCCGAC
GATAAAACTACCGAAGATGCTC
Csa016893
CTTCACAGTCCCATCGTCT
CTCAACGATGACGAAAAGC
Csa016894
GATTTCAATGCCCACGAC
TGACTTTGGCAGCGGTAG
Csa016895
AACTACCAACCTAACCCTGA
CTTGGAGTCTTCATTAGTGCT
Csa016896
AGGTTCCCCTTCTGCGTA
CCATACCGACCTGGAGTTC
Csa016897
ATCAATGACTTGTAATGGGACT
CAAATAATGTTGCGAGTCCT
Csa016898
CAGGGATGGCGAGAAGTG
TTCTTTACTCCCTCTCTTCAGC
Csa016899
AATAAATGCCTGAAAGATGAG
ATGCCATCAACAAACTCTTC
Csa016900
ATGAGCCAAATAACAACCG
CGCCCTTCCCTACACTGA
Csa016901
AAGGCAGCAGGCTATGTG
TGACAATCACCACAAACTCGT
Csa016902
TCCCTACGGTTGAGATTTC
GCTCCTTGACCGTGTATCT
Csa016903
CTGCTTCATCTGGGTTCTAAT
CCACCTCTGATTCCTCCA
Csa016904
TAGATGTGGCTGTTTCGC
CTGGTTTGAGTTGCCTTTT
Csa016905
GAAGAAGGTCCAACAGAGC
CTAAGTCGTCCACTCCCAT
Csa016906
TTAGCGAGGTTCCAGTTAGA
ACTCCTTCCATTCCCTCAA
Csa016907
TATTCAACATTGTCGGCG
AGCAAGATTCAACTCCTGTG
Actin
TCGTGCTGGATTCTGGTG
GGCAGTGGTGGTGAACAT
Note: The primer sequences of cucumber Actin gene were reported in Li et al. (2009).
Supplementary Table S3 The 72 cucumber lines examined for the D/d locus using the SSR markers SSR37 and SSR112
Markes
Lines
Origin
D/G
SSR37
SSR112
S106
America
D
A
S107
America
D
83H
America
83G
Markes
Lines
Origin
D/G
SSR37
SSR112
A
S02
Holand
G
B
B
A
A
S17-2
Holand
G
B
B
D
A
A
S23
Holand
G
B
B
America
D
A
A
S46
Holand
G
B
B
S42
Spain
G
B
B
S46-1
Holand
G
B
B
S75
Spain
G
B
B
S46-2
Holand
D
A
B▲
S76
Spain
G
B
B
S49-1
Holand
G
B
B
M3
China
D
A
A
S49-2
Holand
G
B
B
M12
China
D
A
A
S51-2
Holand
G
B
B
Gl
China
D
A
A
S55-1
Holand
G
B
B
B52
China
D
A
A
S152-11
Holand
D
A
B▲
S52
China
D
B▲
B▲
S159-13
Holand
G
B
B
S57
China
G
B
B
C17-1
Holand
G
B
B
B
C17-2
Holand
G
B
B
S58
China
G
A▲
S59-3
China
G
B
B
S66
Japan
D
A
A
S60
China
D
A
A
S67
Japan
D
A
A
S61-2
China
D
A
A
S69-2
Japan
D
A
A
S80
China
G
B
B
S73-3
Japan
D
A
A
S82
China
G
B
B
S74
Japan
D
A
A
S94
China
D
A
A
S109-6
Japan
D
A
A
S103
China
D
A
A
S118-1
Japan
D
A
A
S105
China
D
A
A
L397-1
Japan
G
B
B
S110
China
D
A
A
S397-20
Japan
G
B
B
S122-2
China
G
B
B
S414
Japan
G
B
B
S122-16
China
G
B
B
S03
Israel
G
B
B
SB-1
China
D
A
A
S05
Israel
G
B
B
C19
France
D
A
B▲
S06
Israel
G
B
B
C21
France
D
A
A
S36
Israel
G
B
B
H34
European
G
B
B
S45
Israel
G
B
B
S34
European
G
B
B
S45-1
Israel
G
B
B
S35-1
European
G
B
B
S45-2
Israel
G
B
B
S124-5
European
D
A
A
S47
Israel
D
A
B▲
S124-6
European
G
B
B
S48-1
Israel
G
B
B
S125-5
European
G
B
B
S48-3
Israel
G
B
B
S126-2
European
G
B
B
S53
Korean
D
A
A
S127-21
European
G
B
B
S112-7
Korean
D
A
A
D: dull fruit phenotype, G: glossy fruit phenotype. Marker genotype designation: A, dull fruit allele; B, glossy fruit allele.
▲ Disagreement
between marker and phenotype.