Scientific Report – 3rd International Workshop on Spectroscopy for
Biology, Maresias, São Paulo, Brazil,
October 18-22th 2010
Organizer
Name (Institution)
Address
Street, Number, Zip-Code
Phone:
E-mail:
Eneida de Paula
UNIVERSIDADE ESTADUAL DE CAMPINAS (UNICAMP)
Instituto de Biologia, Departamento de Bioquímica
Rua Monteiro Lobato, no. 255, Campinas-SP Brasil, CEP 13083-862
+ 55 19 35216143
FAX:
+ 55 19 35216129
depaula@unicamp.br
Co-Organizers (local organizers) Antonio José da Costa Filho, Rosangela Itri, Eneida de Paula,
Leila M. Beltramini
Scientific Advisory Board (SAB) Antonio José da Costa Filho, Rosangela Itri, Fábio Ceneviva
Lacerda Almeida, Leila M. Beltramini, Eneida de Paula
Summary
The International Workshop on Spectroscopy for Biology (IWSB) was a meeting of interdisciplinary
nature, with a view of disseminating, among graduate students and young investigators, cutting edge
techniques used to investigate biologically relevant systems. This workshop served to introduce
students from Brazil and surrounding countries to modern biophysical techniques such as NMR,
fluorescence, ESR, circular dichroism, IR, X-ray scattering, microscopies and molecular simulations.
Furthermore, during this year´s workshop we celebrated Prof. Shirley Schreier´s 70th birthday. Prof.
Schreier is one of the Biophysics pioneers in Brazil, who has always been committed with good
science and education of the students.
This third edition of the workshop had a strong participation of graduate students and post-docs, who
certainly benefit from the close contact with the leading scientist present, a balanced blend of local
and internationally renowned scientists, who presented overviews of their expertises in terms of
biophysical techniques as well as applications in protein structure and conformation, membrane
properties, lipid domains, mechanisms of peptides interaction, biotechnological applications, among
others. The lecturers stood during the entire week of the event, which allowed a deeper interaction
between students and speakers.
IWSB was a nice event in terms of setting the perfect conditions to stimulate scientific discussions and
exchange of state-of-the-art knowledge between young researchers and well-known scientists from
different parts of the world. Moreover, IWSB was an event totally dedicated to modern biophysical
techniques, creating an adequate forum for the Biophysical community in Brazil and Latin America, to
greatly benefit from the scientific program.
The overall evaluation of the Workshop was excellent (77.6%) or good (22.4%) by 51 students that
voluntarily fulfilled the IUPAB evaluation form. The organization, accommodation, quality of
presentations, duration and facilities were equally well evaluated, as summarized below.
The Venue & Location:
Maresias Beach Hotel, São Sebastião, SP, Brasil
The Scientific Programme (attached below)
Changes in the programme: Dr. Paulo Bish could not present his speech so distributed his time and
invited 2 additional participants to give oral presentations (15 min each), namely: Dr. Rosana Chehin
(Argentina) and Dr. Maria Eliana Lanio Ruiz (Cuba).
Invited speakers and lecturers attended at the course:
Ian Smith (National Research Council, Canada)
Wayne Hubbell (University of California Los Angeles, EUA)
Jack Johnson (The Scripps Research Institute, EUA)
Dorothee Kern (Brandeis University, EUA)
Bruno Maggio (Universidad Nacional de Córdoba, Argentina)
Paolo Mariani (Polytechnic University of Marche, Italy)
Francesco Spinozzi (Polytechnic University of Marche, Italy)
Derek Marsh (Max Planck Institute for Biophysical Chemistry, Germany)
Guillermo Montich (Universidad Nacional de Córdoba, Argentina)
Mônica Pickholz (Universidad de Buenos Aires, Argentina)
Manuel Prieto (Universidade Técnica de Lisboa, Portugal)
Alberto Spisni (Universita' degli Studi di Parma, Italy)
Bonnie Wallace (Birkbeck College, University of London, England)
Anthony Watts (University of Oxford, England)
Richard Epand (McMaster University, Canada)
Felix M. Goñi (Universidad del País Vasco, Spain)
Paulo M. Bisch (Federal University of Rio de Janeiro, Brazil)
Antonio J. Costa-Filho (University of São Paulo, Brazil)
Amando S. Ito (University of São Paulo, Brazil)
Maria Cristina Nonato (University of São Paulo, Brazil)
Ana Paula Valente (Federal University of Rio de Janeiro, Brazil)
Pedro G. Pascutti (Federal University of Rio de Janeiro, Brazil)
Karin A. Riske (Federal University of São Paulo, Brazil)
Shirley Schreier (University of São Paulo, Brazil)
Maurício S. Baptista (University of São Paulo, Brazil)
Maria Lucia Bianconi (Federal University of Rio de Janeiro, Brazil)
Maria Teresa Lamy (University of São Paulo, Brazil)
Short oral presentations:
Rosana N. Chehín Instituto Superior de Investigaciones Biológicas (INSIBIO) and Insitituto de
Química Biológica Dr Bernabé Bloj, CONICET-UNT (Argentina)
María Eliana Lanio Ruiz Centro de Estudio de las Proteínas, Facultad de Biología, Universidad de la
Habana (Cuba)
Eduardo M. Clop Depto. Química. FCEFyN, Univ. Nac. de Córdoba (Argentina)
Gianella Facchin Cátedra de Química Inorgánica, DEC, Facultad de Química, UDELAR (Uruguay)
Thiago V. Seraphim Instituto de Química e Instituto de Biologia, Universidade Estadual de Campinas
(Brazil)
Marinônio Lopes Cornélio - Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual
Paulista “Júlio de Mesquita Filho (Brazil)
Leida G. Abraçado Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro (Brazil)
Theo L. F. Souza Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro
Thatyane Nobre Instituto de Física de São Carlos, Universidade de SãoPaulo (Brazil)
Leandro Ramos Souza Barbosa Instituto de Física, Universida de São Paulo (Brazil)
Tatiana Prieto Instituto de Física de São Carlos, Universidade de São Paulo (Brazil)
Sebastião A. Mendanha Instituto de Física, Universidade Federal de Goiás (Brazil)
Jose Carlos Bozelli Junior - Instituto de Química, Universidade de São Paulo (Brazil)
Problems: none
Training aspect: Students were stimulated to participate, making questions after each speech or
lecturer. Besides, there was a “lunch with experts” section, when students were distributed in small
tables (up to 6 places each) where one invited speaker was sitting, to encourage them to talk about
their work.
Key Figures on Participants
The Total Number of regular participants, including plenary speakers but excluding accompanying
guests and local organizers of the Course/Workshop/Special Meeting was:
Total Number
160
Regular Participants Included:
graduate students
74
postdoctoral students
34
principal investigators from academia & industry
20
Number Invited Plenary Speakers:
27
paid by (institution) Support
3
paid by other sponsorship support
24
Number Members of Scientific Advisory/Committee Board
5
Registration fees were paid directly to the Brazilian Biophysics Society that transferred it to the organizers.
Registration fee included hotel costs, meals, course printed materials and coffee breaks
Travel expenses (flight/train tickets) were covered for the lecturers, the tutors and the organizers. Some of the
lecturers needed additional transport assistance to the hotel. This assistance was also covered by the course
budget.
Geographic distribution of participants
8.... participants from Europe & Turkey
8.... participants from USA & Canada
0.... participants from Australia
0.... participants from Asia
0.... participants from Africa
144(8).... participants from South America including (not including) Brazil
The Scientific Presentations Comprised
27..... invited plenary lectures
13.... selected talks or student presentations of 15 minutes
123.... poster presentations
0.... no presentation (visitors & company representative)
Feedback & Comments by Participants (see attached statistics on the questionnaires submitted to
students)
Full summary of comments:
"Very very Nice!
Organization of the program:
"The outstanding work of the organization committee was responsible for the success of the event.
The invited speakers were appropriately selected, because they allowed us to have a very good
overview of the areas."
Questionnaire Summary Form SUBMITTED TO THE STUDENTS
PERCENTUAL RESULTS
EVENT: III International Workshop on Spectroscopy for Biology
Organizers: Eneida de Paula, Rosangela Itri, Antonio José da Costa Filho, Leila Beltramini, Fabio C.L. Almeida
Excellent
Question 1: Organisation of the program.
90,2
Question 2: Quality of Formal Presentations.
74,5
Question 3: Was there adequate discussion after each presentation?
58,8
Question 4: Did the Workshop fulfill your expectations?
70,6
Question 5: Was there…
YES
… sufficient information available about the Workshop?
96,0
Good
9,8
23,5
31,4
29,4
NO
4,0
Adequate
Poor
Unsatisfactory
Sum
51
51
51
51
2,0
9,8
Sum
50
… any problem with transportation?
2,0
98,0
… any problem with the Workshop language?
10,0
90,0
… enough time for informal discussions with all participants?
98,0
2,0
… enough time for discussion after poster sessions?
98,0
2,0
… opportunity for informal discussion with lecturers?
98,0
2,0
… possibility to meet others in your field?
100,0
0,0
… chance to obtain overview of the fields?
98,0
2,0
… chance to receive help with current work?
98,0
2,0
Question 6: Duration of the Workshop?
Right
Too Long
85,7
8,2
Question 7: Location and Accommodation
Excellent
Good
of the Workshop?
70,0
24,0
Question 8: Quality of Facilities?
Excellent
Good
65,2
32,6
Question 9: Give your overall evaluation of the Workshop Excellent
Good
77,6
22,4
Question10: How did you learn about the Workshop?
Posters Web/E-mail
Source
15,3
54,2
50
50
50
50
50
50
51
50
Too Short Should Be
2,0
4,1
Adequate
Poor
4,0
Adequate Poor
0,0
2,2
Adequate
Poor
0,0
0,0
Advert. Mouth
1,7
27,1
days
Sum
49
Unsatisfactory
2
Unsatisfactory
Sum
50
Sum
46
Unsatisfactory
Sum
49
Other
1,7
% of Total
100,0
100,0
100,0
100,0
% of Total
98,0
98,0
98,0
98,0
98,0
98,0
98,0
100,0
98,0
% of Total
96,1
% of Total
98,0
% of Total
90,2
% of Total
96,1
3rd International Workshop on Spectroscopy for Biology October 18, 2010 (Monday)
15:00 – 18:00
18:00 – 18:20
18:20 – 19:20
19:30 – 21:30
9:00 – 10:15
October 19, 2010 (Tuesday)
9:00 – 9:30
Scientific Program
Check-in and Registration
Opening ceremony
Antonio J. Costa-Filho (USP, Brazil), Eneida de Paula (UNICAMP, Brazil)
Fábio C. L. Almeida (UFRJ, Brazil), Leila M. Beltramini (USP, Brazil)
Rosângela Itri (USP, Brazil)
Opening lecture – Chair: Eneida de Paula (UNICAMP, Brazil)
Ian C. P. Smith Institute for Biodiagnostics, National Research Council (Canada)
“Metabolomic Studies of Human Cancer by NMR Spectroscopy”
Dinner
Chair: Antonio J. Costa-Filho (IFSC/USP, Brazil)
Ana P. Valente
Instituto de Bioquímica Médica, Univ. Federal do Rio de Janeiro (Brazil)
“Portrayal of complex dynamic properties of defensins by NMR: multiple motions associated with membrane
interaction”
9:30 – 10:15
Anthony Watts
Biochemistry Department, Oxford University (UK)
“Resolving structural and dynamic details of the binding sites of membrane drug targets at the nanoscale
10:15 – 10:45
Coffee Break
10:45 – 12:00
Chair: Fábio C. L. Almeida (UFRJ, Brazil)
10:45 – 11:00
Eduardo Clop Depto. Química. FCEFyN, Univ. Nac. de Córdoba (Argentina)
“Localization of protonated and deprotonated species of p-Nitrophenol within model membranes”
11:00 – 11:15
Theo L. F. Souza Instituto de Bioquímica Médica, Univ. Federal do Rio de Janeiro (Brazil)
“New Insights to Assist the Drug Design of IAP Antagonists: Structural and Calorimetric Approaches”
11:15 – 12:00
Dorothee Kern Dept. Biochemistry and Howard Hughes Medical Inst., Brandeis Univ. (USA)
“The choreography of a protein’s dance: Exploration by NMR, crystallography, computation and single molecule
fluorescence transfer”
12:00 – 15:00
Lunch
15:00 – 16:15
Chair: Antonio J. Costa-Filho (IFSC/USP, Brazil)
15:00 – 15:15
Sebastião A. Mendanha Instituto de Física, Universidade Federal de Goiás (Brazil)
“Oxidative Stress on the Lipid and Protein Domains of Erythrocyte Membrane Studied by Electron Paramagnetic
Resonance”
15:15 – 15:30
Tatiana Prieto Instituto de Física de São Carlos, Universidade de São Paulo (Brazil)
“Spectroscopy and reactivity studies of hemin-protein complexes”
15:30 – 16:15
Wayne Hubbell Dept of Chemistry and Biochemistry, Univ.California, Los Angeles (USA)
“Exploring protein structure and dynamics with site directed spin labeling”
16:15 – 16:45
Coffee Break
16:45 – 18:00
Chair: Shirley Schreier (IQ/USP, Brazil)
16:45 – 17:15
M. Teresa Lamy Instituto de Física, Universidade de São Paulo (Brazil)
“Structural changes of DMPG vesicle along its anomalous gel-fluid phase transition”
17:15 – 18:00
Derek Marsh Max-Planck-Institut für biophysikalische Chemie (Germany)
“Spin-Label Biospectroscopy”
18:00 – 20:00
Poster Presentation
20:00 – 22:00
Dinner
9:00 – 10:15
Chair: Pietro Ciancaglini (FFCLRP/USP, Brazil)
October 20, 2010 (Wednesday)
9:00 – 9:30
Maurício Baptista Instituto de Química, Universidade de São Paulo (Brazil)
“Membrane biosensors based on SPR: Limits of detection and cooperative interactions of cytochrome C with
membrane mimetics”
9:30 – 10:15
Felix Goñi Fac.Ciencia y Tecnología, Univ. del País Vasco (Spain)
“Novel aspects of detergent interactions with membranes”
10:15 – 10:45
Coffee Break
10:45 – 12:00
Chair: Maria Elisabete D. Zaniquelli (FFCLRP/USP, Brazil)
10:45 – 11:00
Gianella Facchin Facultad de Química, UDELAR (Uruguay)
“Spectroscopic characterization of [Cu(dipeptide)(phenantroline)]
complexes and its interaction with calf thymus DNA”
11:00 – 11:15
Thatyane M. Nobre Instituto de Física de São Carlos, Universidade de São Paulo (Brazil)
“Elucidating Pulchellin intoxication mechanism by using biomembrane model systems”
11:15 – 12:00
Bruno Maggio Facultad de Ciencias Químicas, Universidad Nacional de Córdoba (Argentina)
“The surface topography generated by phosphohydrolytic enzymes revealed by monolayer fluorescence”
12:00 – 15:00
Lunch
15:00 – 16:15
Chair: Thelma A. Pertinhez (Univ. of Parma, Italy)
15:00 – 15:15
Leida Abraçado Instituto de Ciências Biomédicas, Univ. Federal do Rio de Janeiro (Brazil)
“Ferromagnetic resonance and magnetic characteristics of intact magnetosome chains in uncultured Candidatus
magnetoglobus multicellularis”
15:15 – 15:30
Thiago V. Seraphim Instituto de Química, Universidade Estadual de Campinas (Brazil)
“Biochemical and biophysical studies of a novel type I Hsp40 molecular chaperone from sugarcane”
15:30 – 16:15
Alberto Spisni Department of Experimental Medicine, University of Parma (Italy)
16:15 – 16:45
16:45 – 17:45
“Combining structural and functional studies in search for new antimicrobial peptides to be used for human and
animal therapy”
Coffee Break
Chair: Ian C. P. Smith (National Research Council, Canada)
SPECIAL LECTURE: Shirley Schreier Instituto de Química, Univ. de São Paulo (Brazil)
“The magnetism of membranes. A personal account”
Poster Presentation
Dinner
Chair: Eneida de Paula (IB/UNICAMP, Brazil)
18:00 – 20:00
20:00 – 22:00
9:00 – 10:15
October 21, 2010 (Thursday)
9:00- - 9:30
Paulo Bisch Instituto de Biofísica Carlos Chagas Filho, Univ.Federal do Rio de Janeiro (Brazil)
“Peptide–membrane interactions: Molecular modeling and experiments”
9:30 – 10:15
Paolo Mariani Dipartimento SAIFET, Università Politecnica delle Marche (Italy)
“Proteins In solution: an analysis of preferential hydration, structural properties and stability by neutron and X-ray
scattering techniques”
10:15 – 10:45
Coffee Break
10:45 – 12:00
Chair: Rosângela Itri (IF/USP, Brazil)
10:45 – 11:00
María E. Lanio Centro de Estudio de las Proteínas, Fac. de Biología, Univ. Habana (Cuba)
“Sticholysin I mutants and spectroscopic studies reveal novel details of functional mechanism of the actinoporins and
constitute platforms for nanobiotech applications”
11:00 – 11:15
Leandro Barbosa Instituto de Física, Universida de São Paulo (Brazil)
“C12E8 detergent-induced self-assembling of a subunit from Na,K-ATPase: a small angle X-Ray scattering study”
11:15 – 12:00
Francesco Spinozzi Dipartimento SAIFET, Università Politecnica delle Marche (Italy)
“SAXS and SANS analysis of biomolecular systems in solution”
12:00 – 15:00
Lunch
15:00 – 16:15
Chair: Rosângela Itri (IF/USP, Brazil)
15:00 – 15:30
M. Cristina Nonato Fac. Ciências Farmacêuticas de Ribeirão Preto, Univ. São Paulo (Brazil)
“Multiple-approach studies of Dihydroorotate dehydrogenase: A strategy for the treatment of neglected diseases”
15:30 – 16:15
John E. Johnson Department of Molecular Biology, The Scripps Research Institute (USA)
“Time resolved studies of virus maturation: A hybrid methods approach”
16:15 – 16:45
Coffee Break
16:45 – 17:45
Chair: Iseli L. Nantes (UFABC, Brazil)
16:45 – 17:15
M. Lucia Bianconi Instituto de Bioquímica Médica, Univ. Federal do Rio de Janeiro (Brazil)
“Unfolding thermodynamics of the tetrameric l-Asparaginase from E. coli”
17:15 – 17:45
Karin Riske Depto. de Biofísica, Universidade Federal de São Paulo (Brazil)
“Optical microscopy of giant lipid vesicles”
19:00 – 21:00
Dinner
9:00 – 10:15
Chair: Leila M. Beltramini (IFSC/USP, Brazil)
October 22, 2010 (Friday)
9:00 – 9:30
Amando Ito Fac. de Filosofia Ciências e Letras de Ribeirão Preto, Univ. São Paulo (Brazil)
“Fluorescence techniques to study biological model systems”
9:30 – 10:15
Bonnie A. Wallace Dept. of Crystallography and Institute of Structural and Molecular Biology
Birkbeck College, University of London (UK)
“Synchrotron Radiation Circular Dichroism (SRCD) spectroscopy – a new method for examining protein conformation
and protein interactions”
10:15 – 10:45
Coffee Break
10:45 – 12:00
Chair: Eneida de Paula (IB/UNICAMP, Brazil)
10:45 – 11:00
Rosana N. Chehín INSIBIO and Inst. Quím. Biológica Bernabé Bloj, Conicet-UNT (Argentina)
“Glyceraldehyde-3-phosphate dehydrogenase amyloid folding pathway studied by infrared spectroscopy"
11:00 – 11:15
Marinônio L. Cornélio IBILCE, Univ. Estadual Paulista “Júlio de Mesquita Filho (Brazil)
“Monitoring the unfolding transitions of RNase A with differential scanning
calorimetry (DSC) and two-dimensional infrared, sample-sample correlation spectroscopy (SS)”
11:15 – 12:00
Guillermo G. Montich Facultad de Ciencias Químicas, Univ. Nacional de Córdoba (Argentina)
“Conformational changes in the binding of soluble proteins to the lipid membrane interface”
12:00 – 15:00
Lunch
15:00 – 16:15
Chair: Luis Gustavo Dias (FFCLRP/USP, Brazil)
15:00 – 15:30
Pedro G. Pascutti Inst. de Biofisica Carlos Chagas Filho, Univ. Federal Rio de Janeiro (Brazil)
“Molecular simulations applied to enzyme inhibitor development against tropical infectious diseases, proteinmembrane interactions and folding”
15:30 – 16:15
Monica A. Pickholz Fac Pharm & Biochem, University of Buenos Aires (Argentina)
“Molecular dynamics study on the encapsulation of prilocaine in liposomes at physiologic pH”
16:15 – 16:45
Coffee Break
16:45 – 18:00
Closing Ceremony
16:45 – 17:00
Biophysical Society Travel Awards (Chair: Antonio J. Costa-Filho)
17:00 – 17:30
Chair: Rosângela Itri (IF/USP, Brazil)
Iolanda Cuccovia
Instituto de Química, Universidade de São Paulo (Brazil)
17:30 – 18:00
Farewell
19:00- 21:00
Dinner