Table 1 – Primers used during the experimental work
Primer (5’-3’)
Reference/Target
MluIpMCT1
HindIIIpMCT1
MCT1 f1
MCT1 f2
For1:ATGACGCGTGATTGCCTAGAGCTCGTCAG
For2:ATGACGCGTGGACATCAAAGACACTCCATG
Rev :ATGAAGCTTCTGCCTCGTTTGCTTGTTCC
Tm
(ºC)
77.7
78.2
75.7
For1: GATTGCCTAGAGCTCGTCAG
Rev: CTGCCTCGTTTGCTTGTTCC
For2: GGACATCAAAGACACTCCATG
Rev: CTGCCTCGTTTGCTTGTTCC
61.7
67.0
62.0
67.0
RVprimer3
CTAGCAAAATAGGCTGTCCC
GLprimer2
GGAAGACGCCAAAAACATAAAG
STAT3 (MCT1 promoter)
54.1
60.3
For1:GATTACGGTGAGAAAGATTCTGC
Rev1:CCCTTATGTCTCCTCACTGC
62.7
61.3
For2: GTGATCCCCTGAAACGACAATG
Rev2: CTGCAAAAATATCAACCTGAATTCC
FOXM1
MCT1
MCT4
HPRT
56.0
For:GTATTACTCACTAGTAGTACTTGGC
Rev:GAGATTGAAGAAGAGTAGAGATTGG
STAT3 (LDHB promoter)
STAT3
57.0
For:TGTGGGAAGAATCACGCCTTCTAC
Rev:GTCATCCTGGAGATTCTCTACCAC
For:CTTTCTTTGTTTATCAGTGCTGCTAG
Rev:GGCTGGATTTCTTCCTCCTTG
67.5
65.2
69.1
63.9
63.1
65.9
For: GCTGGGCAGTGGTAATTGGA
Rev: CAGTAATTGATTTGGGAAATGCAT
For: CACAAGTTCTCCAGTGCCATTG
Rev: CGCATCCAGGAGTTTGCCTC
For: TGACACTGGCAAAACAATGCA
Rev: GGTCCTTTTCACCAGCAAGCT
67.1
64.4
66.7
69.2
67.5
66.6
Technique
PCR; Automate
sequencing
PCR; Automate
sequencing
PCR; Automate
sequencing
PCR; Automate
sequencing
qRT-PCR
(ChIP)
qRT-PCR
(ChIP)
qRT-PCR
Tm: melting temperature
1