Supplementary table 1 Agronomic and grain quality performance of ten phenotypically selected BC1F1 plants of the
cross ‘HUW234’ × ‘Glu133’ used for background selection
Linel/ Parents
Quality and agronomic traits
GPC
%
Fe (ppm)
Zn
(ppm)
DM
PH
SPN
SPL
TLN
TGW (g)
YPP
(g)
RPG %
14.3
46.0
48.3
110
98
51
9.0
9.0
40.04
7.56
77.3
HUW234-09-36 13.5
49.3
49.8
111
96
51
8.0
8.0
41.20
8.50
79.5
HUW234-09-28 13.7
47.3
49.9
117
95
48
9.0
11
36.84
8.45
70.5
HUW234-09-41 13.9
45.4
45.8
114
92
45
9.0
9.0
38.40
8.20
72.7
HUW234-09-42 13.2
41.3
43.9
110
97
54
10
11
41.60
8.35
75.0
HUW234-09-53 14.2
46.0
48.3
110
98
51
9.0
9.0
41.94
7.85
77.3
HUW234-09-60 13.7
47.3
49.9
117
95
48
9.0
11
36.84
8.23
70.5
HUW234-09-69 13.8
49.3
49.8
111
96
51
8.0
8.0
41.92
8.94
81.8
HUW234-09-72 13.9
45.4
45.8
114
92
45
9.0
9.0
38.40
8.60
72.7
HUW234-09-75 13.2
41.3
43.9
110
97
54
10
11
41.60
7.90
75.0
HUW234
11.15
38.0
28.0
112.6
98.8
51
11
7
91.85
37.3
Glu 133
14
45.2
49
120
82.6
53.6
11
11
42.24
8.41
HUW234-09-8
SE±
0.11
0.88
0.78
0.90
0.68
1.01
0.21
0.4
0.65
0.12
Supplementary table 2 Agronomic and grain quality
HUW234 × Glu133 used for background selection
Parents/plant
GPC % Fe (ppm)
Zn
(ppm)
HUW234-09-69-8
14.6
47.0
48.2
HUW234-09-69-38 14.3
46.0
48.3
HUW234-09-69-19 13.5
49.3
49.8
HUW234-09-69-63 13.4
49.2
49.8
HUW234-09-69-44 13.5
47.3
49.9
HUW234-09-69-14 13.7
47.3
49.4
HUW234-09-69-31 13.6
42.0
42.1
HUW234-09-69-43 13.2
40.7
41.2
HUW234-09-69-12 13.0
40.5
41.4
HUW234-09-69-16 13.0
41.3
47.0
HUW234
11.15
38.0
28.0
Glu 133
14
45.2
49
SE±
0.16
1.12
1.16
performance of ten phenotypically selected BC2F1 plants from
Agronomic and quality traits
DM
PH
SPN
SPL
TLN
109
110
111
112
113
112
114
114
115
115
112.6
120
0.65
9
9
8
8
11
11
13
11
12
11
7
11
0.54
98
98
96
97
95
97
97
97
97
89
98.8
82.6
0.84
51
51
51
50
48
48
50
51
51
57
51
53.6
0.79
9
9
8
8
9
9
10
9
9
11
11
11
0.28
TGW
(gm)
39.2
41.9
41.2
40.2
36.8
36.3
41.4
38.7
38.8
41.45
91.85
42.24
0.63
YPP
(gm)
7.8
8.4
8.3
8.4
8.4
8.2
7.9
8.2
8.3
8.3
37.3
8.41
0.07
RPG
%
91.9
90.7
88.4
88.4
88.4
87.2
87.2
88.4
88.4
88.4
Supplementary table 3 Mean grain quality of selected HUW234*4 × Glu133 BC2F4 progenies
Lines/Parents
Grain quality traits
Fe (ppm)
Zn (ppm)
GPC %
HUW234-09-21
42.0
42.1
13.7
HUW234-09-47
45.2
51.6
13.9
HUW234-09-48
46.0
48.3
14.8
HUW234-09-49
46.3
46.0
13.8
HUW234-09-65
43.6
44.4
13.4
HUW234-09-112
40.5
41.4
13.0
HUW234-09-114
47.3
49.9
13.7
HUW234-09-116
41.3
47.0
13.0
HUW234-09-117
41.2
48.2
13.8
HUW234-09-128
40.3
55.7
13.2
HUW234-09-213
49.3
49.8
13.5
HUW234-09-214
48.4
48.7
13.1
HUW234-09-239
45.4
45.8
13.9
HUW234-09-255
41.3
43.9
13.2
HUW234-09-256
45.4
49.8
13.4
HUW234
38.0
28.0
11.15
Glu 133
45.2
49
14
GPC, grain protein content (%); Fe (ppm),iron content; Zn (ppm), zinc content
Supplementary table 4 Mean agronomic performance of improved HUW234*4 × Glu133 BC2F4
Line/Parent
HUW234-09-21
HUW234-09-47
HUW234-09-48
HUW234-09-49
HUW234-09-65
HUW234-09-112
HUW234-09-114
HUW234-09-116
HUW234-09-117
HUW234-09-128
HUW234-09-213
HUW234-09-214
HUW234-09-239
HUW234-09-255
HUW234-09-256
HUW234
Glu 133
DM
PH (cm)
SPN
SPL
Trait
TLN
TGW (g)
114
110
110
111
111
115
117
115
111
110
111
112
114
110
112
112
120
77.1
89.1
98.2
89.3
93.2
97.4
95.4
89.3
78.9
87.6
96.2
90.8
92.5
97.3
91.7
99.0
82.7
45.2
44.3
51.1
45.3
49.4
47.3
48.1
47.6
49.5
48.2
45.7
49.1
45.9
43.5
52.1
43.6
49.4
10
9
9
11
10
9
9
11
10
8
8
8
9
10
9
11
11
10
10
9
11
12
12
11
11
12
9
8
8
9
11
11
7
11
41.4
40.0
41.9
41.6
42.4
38.8
36.8
41.5
42.0
37.1
41.2
41.8
38.4
41.6
41.8
37.3
41.2
GY
tonnes/ha
4.67
4.57
4.87
4.54
4.57
4.49
4.51
4.52
4.62
4.54
4.47
4.53
4.48
4.39
4.52
4.47
4.63
RPG%
94.2
94.2
94.2
94.2
94.2
94.2
94.2
95.3
95.3
93.2
94.2
93.2
94.2
94.2
95.3
-
DM,days to maturity; PH, plant height; SPN, spikelet number; SPL, spikelet length; TLN, number of tillers; TGW, 1000grain weight;% RPG, per cent recurrent parent genome recovery