mouse IR sequences
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Mouse Insulin Receptor - A (- exon 11) NM_010568 atgggcttcgggagaggatgtgagacgacggctgtgccattgctggtggccgtggccgcgttgctggtgggcacagccggccacctgtaccctggagaggtgtgccctggtatggacatc cggaacaacctgaccaggctacatgagctggagaactgctcagtcattgagggccatctgcagatcctcctgatgttcaagaccagacccgaagatttccgagacctcagtttccccaaa ctcatcatgatcacagattacctgcttctcttccgtgtctatggtctggaaagtctgaaagacctcttcccaaatctcacagtcatccgaggctcccgtctcttcttcaactatgccctggttat cttcgagatggtccacctgaaggagctggggctttataacctcatgaacatcacccggggctctgtccgcatcgagaagaataatgagctctgctacctggccactatcgactggtcccgt atcctggattctgtggaggacaactacattgtactgaacaaagatgacaacgaggaatgtggggatgtctgtccaggcaccgccaagggcaagaccaactgtcctgccactgtcatcaat gggcagtttgtggaacggtgctggacacacagtcattgtcagaaagtttgcccaaccatctgtaagtcacatggctgcacagctgaaggcctgtgctgccacaaagagtgcctgggcaac tgttcggaacctgatgaccccaccaagtgtgtggcctgtcgcaacttctatctggatggtcagtgtgtggagacctgcccgccaccctactatcacttccaggactggcgctgtgtgaacttc agcttctgccaagaccttcacttcaaatgcaggaactctcggaagcctggctgccaccaatacgtcattcacaacaataagtgcatccccgagtgcccgtctggctataccatgaattcca gcaacttgatgtgcaccccatgtctgggaccctgccctaaggtctgccaaatcctcgaaggtgagaagaccattgattctgtgacatctgcccaggagctccgaggctgcactgtgatcaa cggtagcctgatcatcaacatccgagggggcaacaacctggcagctgagctggaggctaaccttggcctcattgaagaaatttcgggatttctaaagatccgccgctcctatgctctggta tcactttctttcttcaggaagctacatctgattcgaggagagaccttggaaattgggaactattctttttatgccttggacaaccagaacctgaggcaactctgggactggagcaaacacaa cctcaccatcactcagggcaagctcttcttccattacaacccgaaactctgcttgtctgaaattcacaagatggaagaagtctccggaactaagggccgtcaggagaggaacgacattgc cctgaagaccaatggggaccaggcatcgtgtgaaaatgaattgcttaaattttctttcattcggacatcttttgacaagatcctgttgaggtgggaaccctactggccccccgacttccgag atctcctgggattcatgttgttctacaaagaggccccttatcagaatgtgacagagtttgatgggcaggatgcttgtggctccaacagctggactgtggtggatattgacccgccccagag gtccaacgaccccaagtctcagaccccaagccaccctgggtggctgatgcggggcctcaaaccctggacccaatacgccatctttgtgaagaccttggttaccttctctgatgaacggcgg acctatggagccaaaagtgatatcatctatgtgcaaacagatgccactaatccttctgtccccctggatcccatatcagtttctaattcctcatctcagattatcttaaagtggaagcccccc tctgaccccaatggcaacatcacacactacctggtgtactgggagaggcaagcagaggacagcgagctgtttgagctggattattgtctcaaagggctgaagctcccttcacggacctgg tccccaccctttgagtctgatgattctcagaagcacaatcagagtgagtatgacgactcggccagtgagtgctgctcatgccctaagactgactctcagatcctgaaggagctggaggagt cttcattcaggaagaccttcgaggattacctgcacaacgtggtttttgtccccaggccatcccgaaagcgaagatcccttgaagaggtggggaatgtgacagccaccacactcacacttcc agatttccccaacgtctcctctaccattgtgcccacaagtcaggaggagcacaggccatttgagaaagtggtgaacaaggagtcacttgtcatctctggcctgagacacttcactgggtac cgcattgagctgcaggcatgcaatcaagattccccagatgagaggtgcagtgtggctgcctacgtcagtgcccggaccatgcctgaagctaaggcagatgacatcgttggccctgtgact catgaaatctttgagaacaatgttgtacacttaatgtggcaagagccaaaggaacctaatggtctgattgtgctatatgaagtgagctatcgccgatatggtgatgaggagctgcacctct gtgtctcccggaagcattttgccctggagcggggctgcaggctgcgagggctctccccaggaaactacagtgttcgagtccgggctacctctctggcaggaaatggctcctggacagaac ccacctatttttatgtgactgattatttagatgtcccatcaaatattgccaaaattatcattggacccctcatctttgtcttcctcttcagtgttgtgattggaagtatttatctatttctgagaaa gaggcagccggatgggccaatgggaccactgtatgcatcttcaaaccctgagtacctcagtgccagtgatgtgtttccatcttctgtgtacgtgccggacgagtgggaggtgcctcgagag aagatcacccttcttcgagagctggggcagggatcctttggtatggtgtatgaaggcaatgccaaggatatcatcaagggtgaggcagagacccgtgttgcggttaagactgtcaatgag tcagccagtcttcgagaacggatcgagttcctcaatgaggcatcagtcatgaagggattcacctgccatcatgtggtccgccttcttggggtggtatccaaaggacagccaacgctggtag tgatggaattgatggctcatggagacctgaaaagtcacctccgttctctgaggccagatgctgagaataacccaggccgccctccccctaccttgcaagaaatgattcagatgacagcag aaattgctgatggcatggcatacttgaacgccaagaagtttgtgcaccgggacctggcagctcgaaactgcatggttgcccatgattttactgtcaaaattggagactttggaatgacaag ggacatctacgagacagattactatcggaaagggggcaagggactgcttcctgtgaggtggatgtcacctgagtccctgaaggatggagtctttactgcttcttctgatatgtggtcctttg gggtggtcctttgggaaatcactagcctggctgagcaaccttatcaaggcctgtctaatgaacaggtgttgaagtttgtcatggatggaggctatctggatccccctgataactgtccagag agactcactgacctgatgcgcatgtgctggcagttcaaccccaagatgaggccaaccttcctggaaatcgtcaacctgctcaaggatgacctccaccccagctttccagaagtttccttctt ctacagcgaggagaacaaggctcctgagagtgaggagctggagatggagtttgaagacatggagaatgtcccgttggatcgttcctctcactgtcagagagaagaggctgggggccgg gagggagggtcctcactgagcatcaaacggacctatgatgaacacatcccctatacccacatgaatgggggcaagaagaacggacgtgtccttaccctgccaaggtcaaacccttccta a Mouse Insulin Receptor - B (+ exon 11) EU346716.1 atgggcttcgggagaggatgtgagacgacggctgtgccattgctggtggccgtggccgcgttgctggtgggcacagccggccacctgtaccctggagaggtgtgccctggtatggacatc cggaacaacctgaccaggctacatgagctggagaactgctcagtcattgagggccatctgcagatcctcctgatgttcaagaccagacccgaagatttccgagacctcagtttccccaaa ctcatcatgatcacagattacctgcttctcttccgtgtctatggtctggaaagtctgaaagacctcttcccaaatctcacagtcatccgaggctcccgtctcttcttcaactatgccctggttat cttcgagatggtccacctgaaggagctggggctttataacctcatgaacatcacccggggctctgtccgcatcgagaagaataatgagctctgctacctggccactatcgactgg tcccgtatcctggattctgtggaggacaactacattgtactgaacaaagatgacaacgaggaatgtggggatgtctgtccaggcaccgccaagggcaagaccaactgtcctgccactgtc atcaatgggcagtttgtggaacggtgctggacacacagtcattgtcagaaagtttgcccaaccatctgtaagtcacatggctgcacagctgaaggcctgtgctgccacaaagagtgcctg ggcaactgttcggaacctgatgaccccaccaagtgtgtggcctgtcgcaacttctatctggatggtcagtgtgtggagacctgcccgccaccctactatcacttccaggactggcgctgtgt gaacttcagcttctgccaagaccttcacttcaaatgcaggaactctcggaagcctggctgccaccaatacgtcattcacaacaataagtgcatccccgagtgcccgtctggctatacc atgaattccagcaacttgatgtgcaccccatgtctgggaccctgccctaaggtctgccaaatcctcgaaggtgagaagaccattgattctgtgacatctgcccaggagctccgaggctgca ctgtgatcaacggtagcctgatcatcaacatccgagggggcaacaacctggcagctgagctggaggctaaccttggcctcattgaagaaatttcgggatttctaaagatccgccgctcc tatgctctggtatcactttctttcttcaggaagctacatctgattcgaggagagaccttggaaattgggaactattctttttatgccttggacaaccagaacctgaggcaactctgggactgg agcaaacacaacctcaccatcactcagggcaagctcttcttccattacaacccgaaactctgcttgtctgaaattcacaagatggaagaagtctccggaactaagggccgtcaggag aggaacgacattgccctgaagaccaatggggaccaggcatcgtgtgaaaatgaattgcttaaattttctttcattcggacatcttttgacaagatcctgttgaggtgggaaccctactggcc ccccgacttccgagatctcctgggattcatgttgttctacaaagaggccccttatcagaatgtgacagagtttgatgggcaggatgcttgtggctccaacagctggactgtggtggat attgacccgccccagaggtccaacgaccccaagtctcagaccccaagccaccctgggtggctgatgcggggcctcaaaccctggacccaatacgccatctttgtgaagaccttggttacc ttctctgatgaacggcggacctatggagccaaaagtgatatcatctatgtgcaaacagatgccactaatccttctgtccccctggatcccatatcagtttctaattcctcatctcagatt atcttaaagtggaagcccccctctgaccccaatggcaacatcacacactacctggtgtactgggagaggcaagcagaggacagcgagctgtttgagctggattattgtctcaaagggct gaagctcccttcacggacctggtccccaccctttgagtctgatgattctcagaagcacaatcagagtgagtatgacgactcggccagtgagtgctgctcatgccctaagactgactctcag atcctgaaggagctggaggagtcttcattcaggaagaccttcgaggattacctgcacaacgtggtttttgtccccagAAAAACCTCTTCAGGCAATGGTGCCGAGGACAG TAGgccatcccgaaagcgaagatcccttgaagaggtggggaatgtgacagccaccacactcacacttccagatttccccaacgtctcctctaccattgtgcccacaagtcaggaggagc acaggccatttgagaaagtggtgaacaaggagtcacttgtcatctctggcctgagacacttcactgggtaccgcattgagctgcaggcatgcaatcaagattccccagatgagaggtgca gtgtggctgcctacgtcagtgcccggaccatgcctgaagctaaggcagatgacatcgttggccctgtgactcatgaaatctttgagaacaatgttgtacacttaatgtggcaagagccaa aggaacctaatggtctgattgtgctatatgaagtgagctatcgccgatatggtgatgaggagctgcacctctgtgtctcccggaagcattttgccctggagcggggctgcaggctgcgagg gctctccccaggaaactacagtgttcgagtccgggctacctctctggcaggaaatggctcctggacagaacccacctatttttatgtgactgattatttagatgtcccatcaaatattgccaa aattatcattggacccctcatctttgtcttcctcttcagtgttgtgattggaagtatttatctatttctgagaaagaggcagccggatgggccaatgggaccactgtatgcatcttcaaaccct gagtacctcagtgccagtgatgtgtttccatcttctgtgtacgtgccggacgagtgggaggtgcctcgagagaagatcacccttcttcgagagctggggcagggatcctttggtatggtgta tgaaggcaatgccaaggatatcatcaagggtgaggcagagacccgtgttgcggttaagactgtcaatgagtcagccagtcttcgagaacggatcgagttcctcaatgaggcatcagtca tgaagggattcacctgccatcatgtggtccgccttcttggggtggtatccaaaggacagccaacgctggtagtgatggaattgatggctcatggagacctgaaaagtcacctccgttctct gaggccagatgctgagaataacccaggccgccctccccctaccttgcaagaaatgattcagatgacagcagaaattgctgatggcatggcatacttgaacgccaagaagtttgtgcacc gggacctggcagctcgaaactgcatggttgcccatgattttactgtcaaaattggagactttggaatgacaagggacatctacgagacagattactatcggaaagggggcaagggactg cttcctgtgaggtggatgtcacctgagtccctgaaggatggagtctttactgcttcttctgatatgtggtcctttggggtggtcctttgggaaatcactagcctggctgagcaaccttatcaag gcctgtctaatgaacaggtgttgaagtttgtcatggatggaggctatctggatccccctgataactgtccagagagactcactgacctgatgcgcatgtgctggcagttcaaccccaag atgaggccaaccttcctggaaatcgtcaacctgctcaaggatgacctccaccccagctttccagaagtttccttcttctacagcgaggagaacaaggctcctgagagtgaggagctggag atggagtttgaagacatggagaatgtcccgttggatcgttcctctcactgtcagagagaagaggctgggggccgggagggagggtcctcactgagcatcaaacggacctatgatgaaca catcccctatacccacatgaatgggggcaagaagaacggacgtgtccttaccctgccaaggtcaaacccttcctaa Initial Set of Primers (IR F & IR R) mouse IR forward primer within exon 10 taagactgactctcagatcctg (22bp, Tm 60.44) (gc = 10 & at = 12, calculated Tm 64 4x10 + 2x12) mouse IR reverse primer within exon 12 gac aag tga ctc ctt gtt cac (21bp, Tm 61.14) gtgaacaaggagtcacttgtc PCR Fragment obtained using: mouseIR_forward1 & mouseIR_reverse1 from matrix DNA <mouse_IR-A.xdna> taagactgactctcagatcctgaaggagctggaggagtcttcattcaggaagaccttcgaggattacctgcacaacgtggtttttgtccccaggccatcccgaaagcgaagatcccttgaa gaggtggggaatgtgacagccaccacactcacacttccagatttccccaacgtctcctctaccattgtgcccacaagtcaggaggagcacaggccatttgagaaagtggtgaacaagga gtcacttgtc 250 bp PCR Fragment obtained using: mouseIR_forward1 & mouseIR_reverse1 from matrix DNA <mouse_IR-B.xdna> taagactgactctcagatcctgaaggagctggaggagtcttcattcaggaagaccttcgaggattacctgcacaacgtggtttttgtccccagAAAAACCTCTTCAGGCAATGG TGCCGAGGACAGTAGgccatcccgaaagcgaagatcccttgaagaggtggggaatgtgacagccaccacactcacacttccagatttccccaacgtctcctctaccattgtgccc acaagtcaggaggagcacaggccatttgagaaagtggtgaacaaggagtcacttgtc 286 bp Second Set of Primers (IR exon F & IR exon R) mouse IR exon 9-10 forward primer bridging across exon 9-10 ttgtctcaaagggctgaagc (20bp, GC% 50) Tm 60 mouse IR exon 12-13 reverse primer bridging across exon 12-13 gtc atc tgc ctt agc ttc agg (21bp, GC% 52) Tm 62.57 cctgaagctaaggcagatgac PCR Fragment obtained using: mouseIR_ex 9-10 F & mouseIR_ex 12-13 R from matrix DNA <mouse_IR-A.xdna> ttgtctcaaagggctgaagctcccttcacggacctggtccccaccctttgagtctgatgattctcagaagcacaatcagagtgagtatgacgactcggccagtgagtgctgctcatgccct aagactgactctcagatcctgaaggagctggaggagtcttcattcaggaagaccttcgaggattacctgcacaacgtggtttttgtccccaggccatcccgaaagcgaagatcccttgaa gaggtggggaatgtgacagccaccacactcacacttccagatttccccaacgtctcctctaccattgtgcccacaagtcaggaggagcacaggccatttgagaaagtggtgaacaagga gtcacttgtcatctctggcctgagacacttcactgggtaccgcattgagctgcaggcatgcaatcaagattccccagatgagaggtgcagtgtggctgcctacgtcagtgcccggaccatg cctgaagctaaggcagatgac 502 bp PCR Fragment obtained using: mouseIR_ex 9-10 F & mouseIR_ex 12-13 R from matrix DNA <mouse_IR-B.xdna> ttgtctcaaagggctgaagctcccttcacggacctggtccccaccctttgagtctgatgattctcagaagcacaatcagagtgagtatgacgactcggccagtgagtgctgctcatgccct aagactgactctcagatcctgaaggagctggaggagtcttcattcaggaagaccttcgaggattacctgcacaacgtggtttttgtccccagAAAAACCTCTTCAGGCAATGGT GCCGAGGACAGTAGgccatcccgaaagcgaagatcccttgaagaggtggggaatgtgacagccaccacactcacacttccagatttccccaacgtctcctctaccattgtgccca caagtcaggaggagcacaggccatttgagaaagtggtgaacaaggagtcacttgtcatctctggcctgagacacttcactgggtaccgcattgagctgcaggcatgcaatcaagattccc cagatgagaggtgcagtgtggctgcctacgtcagtgcccggaccatgcctgaagctaaggcagatgac 538 bp Third Set of Primers (rat IR splice F & rat IR splice R) rat IR splice forward ttcattcaggaagaccttcga (21 bp) rat IR splice reverse cag gcc aga gat gac aag tga c (22 bp) gtcacttgtcatctctggcctg PCR Fragment obtained using: rat IR splice F & rat IR splice R from matrix DNA <mouse_IR-A.xdna> ttcattcaggaagaccttcgaggattacctgcacaacgtggtttttgtccccaggccatcccgaaagcgaagatcccttgaagaggtggggaatgtgacagccaccacactcacacttcc agatttccccaacgtctcctctaccattgtgcccacaagtcaggaggagcacaggccatttgagaaagtggtgaacaaggagtcacttgtcatctctggcctg 223 bp PCR Fragment obtained using: rat IR splice F & rat IR splice R from matrix DNA <mouse_IR-B.xdna> ttcattcaggaagaccttcgaggattacctgcacaacgtggtttttgtccccagAAAAACCTCTTCAGGCAATGGTGCCGAGGACAGTAGgccatcccgaaagcgaag atcccttgaagaggtggggaatgtgacagccaccacactcacacttccagatttccccaacgtctcctctaccattgtgcccacaagtcaggaggagcacaggccatttgagaaagtggt gaacaaggagtcacttgtcatctctggcctg 259 bp
