CENTRE FOR GENOME-ENABLED BIOLOGY AND MEDICINE CGEBM Booking Form Please complete Part A of this form and submit it to CEGBM via email to: e.collieduguid@abdn.ac.uk. Please allow up to 1 week for consideration and return of the assessment to you. Please indicate in your email if your grant deadline requires an earlier response. Part A - To be completed by the applicant Name of PI PI contact details (full postal, telephone, and email) Affiliation (School/Institute) (internal only) Services Required NGS Microarray Bioinformatics Biostatistics Data Management Data Storage Access to HPCC (Maxwell) Study title Description of study Does this study require Ethical Approval? Ethics Ref No R&D ref No Yes/ No Data generated by CGEBM will be stored on University of Aberdeen secure network drives. Data analysed within the CGEBM bioinformatics unit will also be transferred to the University of Aberdeen HPC, Maxwell. CGEBM has no responsibility to obtain regulatory approvals for data storage and this is the sole responsibility of the PI named above. Please stipulate if you require alternative data storage and provide details here: Funding body Study type (e.g. pilot, project grant, programme grant, fellowship) Submission deadline Project start date Duration of the project Proposed CGEBM start date Consultation date (CGEBM use) GMO Material provided GMO Approval CGEBM Project Form v4 Yes/ No GMO/ cellular constituent Copy provided (Approval and Handling SOP)/ Not applicable 16 January 2014 Page 1 of 4 CENTRE FOR GENOME-ENABLED BIOLOGY AND MEDICINE CGEBM Booking Form NGS (if NGS selected above, the applicant should complete this section) Organism (common name) Genus/ Species Reference sequence for organism (Yes/No) Size of target region e.g. Genome, Transcriptome, amplicon Ploidy (e.g. Haploid, diploid, etc) Application (e.g. WGS, WES, RNAseq, ChIPseq, BISseq, RADseq, metagenome, etc) Principal Aim (e.g. differential gene expression, SNP/ variant calling, de novo assembly, miRNA, methylated DNA, microbial diversity, etc) Number of samples Starting material (e.g. genomic DNA, amplicon, total RNA, mRNA, ChIP DNA, cells etc) NOTE: we can only process cells/ tissues of types covered by our containment levels/ approvals Sample Status (Snap frozen, in TRIzol, in RNALater, etc) Is this work part of a larger study with existing NGS data that needs to be considered in the design and/or choice of platform? Bioinformatics required Yes/ No Biostatistics required Yes/ No If you have preferences for sequencing design specifications please add the relevant details below in Part B, otherwise this section will be completed by CGEBM. Microarrays (if Microarray selected above, the applicant should complete this section) Organism (common name) Genus/ Species Application (gene expression, genotyping, etc) Principal Aim (e.g. differential gene expression, SNP/ variant calling, miRNA expression, etc) Number of samples Starting material (e.g. genomic DNA, total RNA, mRNA, cells, tissues etc) NOTE: we can only process cells/ tissues of types covered by our containment levels/ approvals Sample Status (Snap frozen, in TRIzol, in RNALater, etc) Service Required (e.g. HYB only; Cells to Chip; Cells to QC’d data, etc) Microarray required Target Preparation Kit (If prepared by user, please state) Poly A controls used (Where applicable, for user prepared Yes/ No Volume (ul) samples) Is this work part of a larger study with existing microarray data that needs to be considered in the design and/or choice of microarray? Bioinformatics required Biostatistics required CGEBM Project Form v4 Yes/ No Yes/ No 16 January 2014 Page 2 of 4 CENTRE FOR GENOME-ENABLED BIOLOGY AND MEDICINE CGEBM Booking Form Part B – CGEBM use only Technical assessment of project CGEBM Reference For NGS NGS Platform (Ion Proton/Illumina MiSeq) Paired-end or single read Coverage/Depth Read length Barcoded libraries Library preparation For Microarray Microarray details above complete Date received by CGEBM Technical assessment of project by: CGEBM Description of work to be performed NGS Costs Biostatistics costs Bioinformatics costs Microarray costs Data storage costs Data Management costs Miscellaneous costs Access to HPCC (Maxwell) Currently free at point of use. If funding available from the funding body for this type of infrastructure, please discuss options with IT Total costs CGEBM Project Form v4 16 January 2014 Page 3 of 4 CENTRE FOR GENOME-ENABLED BIOLOGY AND MEDICINE CGEBM Booking Form Please wait to sign this form until CGEBM has assessed your requirements and returned the form to you with a proposal of the work to be performed. To be completed and signed by PI prior to CGEBM commencing project: NGS project Date Samples Delivered No. Samples Sample Names Grouping Information (e.g. Group 1: Control – Samples 2, 4, 6; Group 2: timepoint 1 – Samples T1S2, T1S4, T1S6; Group 3 – timepoint 2 – Samples T2S2, T2S4, T2S6) Sequencing (wet lab to QC’d raw data) Date results required Expected Completion Date (Provided by CGEBM) Completion Date (CGEBM use only) Analysis: Bioinformatics/ biostatistics Date results required Expected Completion Date (Provided by Bioinformatics) Completion Date (CGEBM use only) Microarray project Date Samples Delivered No. Samples Sample Names Grouping Information (e.g. Group 1: Control – Samples 2, 4, 6; Group 2: timepoint 1 – Samples T1S2, T1S4, T1S6; Group 3 – timepoint 2 – Samples T2S2, T2S4, T2S6) Microarrays (wet lab to QC’d raw data) Date results required Expected Completion Date (Provided by CGEBM) Completion Date (CGEBM use only) Analysis: Bioinformatics/ biostatistics Date results required Expected Completion Date (Provided by Bioinformatics) Completion Date (CGEBM use only) By signing this form you are agreeing to CGEBM staff performing the services specified in this Technical Assessment above, at the quoted cost and under our Terms and Conditions Signature: Date: CENTRE FOR GENOME-ENABLED BIOLOGY AND MEDICINE University of Aberdeen, 23 St. Machar Drive, Aberdeen, Scotland, UK AB24-3RY Email: e.collie-duguid@abdn.ac.uk Tel: +44(0)1224 437958 The University of Aberdeen is a charity registered in Scotland, No SC013683 CGEBM Project Form v4 16 January 2014 Web: http://www.abdn.ac.uk/genomics/ Page 4 of 4