Supplementary Online Data
Supplementary Online Table S1
3-way nested ANOVAs, showing summary statistics for development period (DP), body weight
(BW), adult longevity (AL), male song pulse pair rate (PR), male song asynchrony interval (AI),
male song attractiveness index (AT), and female pulse-pair rate threshold.
Maryland population;
males
Environmental
Source
d.f.
DP-1
log (BW)
Gradient
Temperature
F
MS (x10-2)
F
Condition
1
1181.02
736.61***
1485.82
795.27*
Sire
23
1.81
0.83
3.39
1.61
Dam (sire)
48
1.03
2.04**
1.44
1.17
Condition x Sire
23
1.66
3.33***
1.91
1.56
Condition x Dam (Sire)
48
0.5
1.66**
1.23
1.38*
686a
0.3
Condition
1
21.08
44.28***
4.55
8.01**
Sire
23
2.61
3.1**
3.46
2.77**
Dam (sire)
48
1.91
0.78*
1.51
1.89*
Condition x Sire
23
0.49
1.21
0.56
0.71
Error
Density
MS (x10-5)
6.37
Condition x Dam (Sire)
Error
ad.f.
48
0.41
806b
0.23
1.75**
0.80
1.12
0.72
for BW, AL, PR, PA, AI, and AT are 683,591,584,585,581 and 585, respectively.
b
d.f. for BW, AL, PR, PA, AI, and A
*, **, *** : P < 0.05, 0.01, 0.001, respectively.
Maryland population;
Females
Environmental
Source
d.f.
DP-1
log (BW)
Gradient
Temperature
F
MS
F
Condition
1
745.17
1268.66 ***
43334.63
848.39*
Sire
23
1.08
1.20
73.42
1.45
Dam (sire)
47
0.55
2.08**
26.52
0.90
Condition x Sire
23
0.62
2.34**
53.35
1.83*
Condition x Dam (Sire)
47
0.26
1.28
29.39
1.49*
690a
0.21
Condition
1
17.84
29.18***
480.11
22.34**
Sire
23
1.48
1.46
79.85
2.28*
Dam (sire)
48
0.69
2.29**
33.67
1.73*
Error
Density
MS (x10-5)
19.71
Condition x Sire
23
0.63
2.12*
21.39
1.09
Condition x Dam (Sire)
48
0.3
1.44
19.51
0.83
700b
0.23
Error
ad.f.
for BW, AL, PR threshold are 687, 613 and 632, respectively.
b
23.47
d.f. for BW, AL and PR threshold are 700, 588 and 6
Louisiana population;
Males
Environmental
Source
d.f.
DP-1
log (BW)
Gradient
MS (x10-5)
F
MS (x10-2)
F
Temperature
Condition
1
1275.84
511.18***
775.93
1281.99***
Sire
13
2.04
0.6
2.62
9.92
Dam (sire)
27
1.97
1.85
0.76
0.69
Condition x Sire
13
2.54
2.42
0.60
0.55
Condition x Dam (Sire)
27
1.06
1.60
1.10
1.99
506a
0.66
Condition
1
85.61
209.81***
0.88
0.65
Sire
13
2.46
2.71
5.39
2.40*
Dam (sire)
27
1.52
1.50
1.89
1.91*
Condition x Sire
13
0.41
0.40
1.36
1.38
Condition x Dam (Sire)
27
1.01
2.11
0.99
1.36
541b
0.48
Error
Density
Error
ad.f.
0.56
for BW, AL, PR, PA, AI, and AT are 503, 449, 485, 484 and 485, respectively.
*, **, *** : P < 0.05, 0.01, 0.001, respectively.
DP: development time
BW: body weight
AL: adult longevity
0.73
b
d.f. for BW and AL are 537, 475 resp
Louisiana population;
females
Environmental
Source
d.f.
DP-1
log (BW)
Gradient
Temperature
MS
F
1
672.26
367.36***
14096.16
397.11***
Sire
13
1.72
0.82
85.7
2.21
Dam (sire)
27
0.75
1.44
22.72
1.14
Condition x Sire
13
1.88
3.63**
36.08
1.82
Condition x Dam (Sire)
27
0.52
1.27
19.90
1.19
412a
0.41
Condition
1
104.15
6.34*
107.38
3.95
Sire
13
55.32
3.70
74.50
2.75
Dam (sire)
28
13.55
0.89
20.43
0.96
Condition x Sire
13
16.52
1.09
27.59
1.31
Condition x Dam (Sire)
28
15.17
1.12
21.29
1.31
466b
13.56
Error
ad.f.
F
Condition
Error
Density
MS (x10-5)
for BW, AL, PR threshold are 411, 345 and 372, respectively.
respectively.
b
16.75
16.30
d.f. for BW, AL and PR threshold are 466, 375, 409
M
Supplementary Online Table S2
3-way nested ANOVAs for each of 4 environmental rearing conditions; see Supplementary Online Table S1 for
Male traits
Environment
DP-1
log (BW)
AL
Source
d.f.
MS (x10-5)
F
d.f.
MS (x10-2)
F
d.f.
MS
Population
1
76.99
37.46***
1
5.33
3.85
1
0.58
High
Sire (population)
36
2.14
2.35***
36
1.43
1.49
36
17.65
temperature
Dam (population, sire)
75
0.94
2.29***
75
0.97
1.45*
75
5.537
Error
579
0.41
576
0.67
506
3.52
Population
1
6.00
3.33
1
12.42
3.55
1
803.50
Sire (population)
36
1.79
1.73*
36
3.6
2.45***
36
46.08
Low
temperature
Dam (population, sire)
76
1.06
Error
617
0.9
Population
1
2.95
High
Sire (population)
36
Density
Dam (population, sire)
Error
2.16***
76
1.4
614
0.82
1.89
1
0.00
1.55
1.76*
36
76
0.90
2.33***
657
0.39
Population
1
57.03
Low
Sire (population)
36
Density
Dam (population, sire)
Error
1.75***
76
21.85
540
15.61
0.00
1
2.82
2.87
1.65*
36
22.96
76
1.78
2.05***
76
13.763
651
0.87
573
7.57
39.74***
1
8.95
4.29*
1
13.72
1.47
1.81*
36
2.13
2.56***
36
30.56
76
0.84
2.97***
76
0.58
0.84**
76
10.25
690
0.28
604
6.84
688
*, **, *** : P < 0.05, 0.01, 0.001,
respectively.
Female traits
Environment
DP-1
log (BW)
AL
Source
High
d.f.
MS (x10-5)
F
d.f.
MS
F
d.f.
MS
Population
1
66.70
55.25***
1
20.45
0.88
1
1.45
Sire (population)
36
1.30
2.00**
36
24.59
1.66*
36
6.81
temperature
Dam (population, sire)
75
0.66
Error
600
0.32
Population
1
7.28
Low
Sire (population)
36
temperature
Dam (population, sire)
Error
2.07***
75
14.89
599
11.92
6.87*
1
2857.58
1.09
3.11***
36
76
0.36
1.53**
511
0.23
Population
1
4.50
High
Sire (population)
36
Density
Dam (population, sire)
Error
1.25
75
2.452
516
2.59
30.37***
1
6.24
96.48
2.71***
36
23.88
76
36.08
1.37*
76
10.688
508
26.26
442
9.38
3.53
1
1062.91
19.59***
1
1.03
1.33
2.40**
36
55.97
2.01**
36
10.37
76
0.57
1.69**
76
28.03
1.08
76
6.159
567
0.34
567
25.90
467
6.18
Population
1
29.51
35.58***
1
2.72
0.06
1
44.07
Low
Sire (population)
36
0.84
1.66*
36
49.44
2.52***
36
7.80
Density
Dam (population, sire)
76
0.52
1.92***
76
19.79
1.25
76
5.95
Error
595
0.27
595
15.81
493
4.32
*, **, *** : P < 0.05, 0.01, 0.001,
respectively.
Supplementary Online Table S3 : Heritability of female and male life history traits in 2 A. grisella
populations reared under 4 environmental conditions. h2 values represent maximum likelihood
estimates made from transformed data (see text). LRT1 and LRT2 represent likelihood ratio
tests for significance of heritability estimate and of non-additive genetic variance, respectively,
evaluated assuming a Χ2 distribution with d.f. = 1; boldface : p < 0.05; underline : P < 0.05
following Holm correction for multiple tests.
Trait
Environment Population h2 ± se
LRT1 LRT2
Female weight
High temp.
Low temp.
High density
Low density
Female development period
High temp.
Low temp.
High density
Maryland
Louisiana
Maryland
Louisiana
Maryland
Louisiana
Maryland
Louisiana
0.10 ± 0.05
0.15 ± 0.07
0.35 ± 0.09
0.37 ± 0.11
0.08 ± 0.05
0.11 ± 0.06
0.17 ± 0.06
0.26 ± 0.09
3.42
4.53
27.31
23.26
2.55
2.92
11.91
14.44
0.82
0.00
0.00
0.00
0.00
0.00
0.00
0.00
Maryland
Louisiana
Maryland
Louisiana
Maryland
0.46 ± 0.09
0.46 ± 0.12
0.52 ± 0.11
0.37 ± 0.11
0.69 ± 0.12
40.60
22.33
52.94
25.79
69.63
0.77
0.06
0.02
0.00
0.55
Low density
Female adult longevity
High temp.
Low temp.
High density
Low density
Male weight
High temp.
Low temp.
High density
Low density
Male development period
High temp.
Low temp.
High density
Low density
Male adult longevity
High temp.
Low temp.
High density
Low density
Louisiana
Maryland
Louisiana
0.21 ± 0.08
0.44 ± 0.09
0.29 ± 0.09
11.40
36.76
14.04
0.00
0.12
0.74
Maryland
Louisiana
Maryland
Louisiana
Maryland
Louisiana
Maryland
Louisiana
0.20 ± 0.06
0.07 ± 0.06
0.23 ± 0.08
0.16 ± 0.08
0.13 ± 0.06
0.09 ± 0.07
0.10 ± 0.05
0.19 ± 0.08
13.68
0.84
11.44
4.44
5.22
1.74
3.10
6.03
0.00
0.00
0.00
0.00
0.00
0.03
0.12
0.00
Maryland
Louisiana
Maryland
Louisiana
Maryland
Louisiana
Maryland
Louisiana
0.19 ± 0.06
0.16 ± 0.07
0.38 ± 0.08
0.31 ± 0.09
0.29 ± 0.07
0.44 ± 0.11
0.25 ± 0.06
0.36 ± 0.10
8.16
5.36
34.05
16.57
22.63
21.99
18.77
33.54
0.16
0.16
0.00
0.04
0.62
1.32
0.07
0.00
Maryland
Louisiana
Maryland
Louisiana
Maryland
Louisiana
Maryland
Louisiana
0.67 ± 0.12
0.51 ± 0.13
0.67 ± 0.12
0.26 ± 0.08
0.56 ± 0.10
0.33 ± 0.09
0.70 ± 0.11
0.53 ± 0.13
75.03
31.54
64.14
9.27
86.91
13.25
112.23
27.53
0.17
0.76
0.40
2.63
0.08
2.48
0.16
3.36
Maryland
Louisiana
Maryland
Louisiana
Maryland
Louisiana
Maryland
Louisiana
0.52 ± 0.11
0.30 ± 0.10
0.36 ± 0.09
0.19 ± 0.08
0.46 ± 0.10
0.10 ± 0.06
0.47 ± 0.09
0.21 ± 0.08
54.75
13.50
31.51
6.48
41.82
1.43
50.08
10.70
0.00
0.00
0.00
0.00
0.10
2.36
0.00
0.00
Supplementary Online Table S4 : . Genetic covariance (Cov) and standard error of covariance
between female and male traits in 2 A. grisella populations reared under 4 environmental
conditions. Values represent maximum likelihood estimates made from transformed data (see
text). LRT represents likelihood ratio test statistic for significance of genetic correlation estimate
(rg), evaluated assuming a Χ2 distribution with d.f. = 1; boldface : p < 0.05; underline : p < 0.05
following Holm correction for multiple tests.
Environ. rearing cond.; 0: low temperature; 1: high density; 2: high temp.; 3: low density
Population; 0 : Maryland; 1 : Louisiana
Female trait; 0: development time; 1: body weight; 2: adult longevity; 3: pulse-pair rate
response
Male trait; 0: development time; 1: body weight; 2 : adult longevity; 3: song pulse-pair
rate; 4: song peak amplitude; 5: song asynchrony interval; 6: song attractiveness
index
Environmental
rearing condition
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
Population
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
1
1
1
1
1
1
1
1
1
Female
trait
0
0
0
0
0
0
0
1
1
1
1
1
1
1
2
2
2
2
2
2
2
3
3
3
3
3
3
3
0
0
0
0
0
0
0
1
1
1
Male
trait
0
1
2
3
4
5
6
0
1
2
3
4
5
6
0
1
2
3
4
5
6
0
1
2
3
4
5
6
0
1
2
3
4
5
6
0
1
2
Cov
s.e. (Cov)
0.51
-0.31
-0.21
-0.05
-0.22
-0.01
-0.23
-0.30
0.34
0.21
-0.12
0.28
0.03
0.14
-0.16
0.17
0.30
-0.06
0.04
0.00
0.01
0.08
-0.05
-0.08
-0.05
-0.01
0.02
-0.02
0.10
0.02
0.05
-0.07
0.04
-0.03
-0.01
-0.25
0.24
0.14
0.09
0.07
0.07
0.07
0.07
0.05
0.06
0.07
0.06
0.06
0.06
0.06
0.04
0.05
0.07
0.06
0.06
0.06
0.05
0.04
0.05
0.07
0.05
0.06
0.06
0.05
0.04
0.04
0.07
0.07
0.06
0.06
0.06
0.05
0.07
0.07
0.08
0.07
LRT
rg
24.51
11.26
4.90
0.23
6.36
0.06
10.98
9.48
19.97
6.42
1.82
16.06
0.34
4.81
2.83
4.93
16.12
0.58
0.39
0.00
0.05
0.77
0.39
1.22
0.40
0.01
0.20
0.16
1.03
0.03
0.35
0.71
0.23
0.16
0.01
7.22
5.93
2.56
0.86
-0.69
-0.46
-0.12
-0.60
-0.99
-0.89
-0.64
1.00
0.58
-0.34
0.91
1.00
0.66
-0.38
0.56
1.00
-0.22
0.18
0.93
0.08
0.23
-0.18
-0.31
-0.19
-0.04
1.00
-0.14
0.31
0.05
0.20
-0.30
0.18
-0.37
-0.03
-0.81
0.63
0.50
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
1
1
1
1
1
1
1
1
1
1
1
1
1
2
2
2
2
2
2
2
3
3
3
3
3
3
3
0
0
0
0
0
0
0
1
1
1
1
1
1
1
2
2
2
2
2
2
2
3
3
3
3
3
3
3
0
0
0
0
0
0
0
1
1
1
3
4
5
6
0
1
2
3
4
5
6
0
1
2
3
4
5
6
0
1
2
3
4
5
6
0
1
2
3
4
5
6
0
1
2
3
4
5
6
0
1
2
3
4
5
6
0
1
2
3
4
5
6
0
1
2
0.10
0.02
0.00
0.08
-0.22
0.22
0.19
0.10
0.15
0.04
0.21
0.18
-0.07
0.07
-0.01
-0.09
0.02
-0.09
0.62
-0.02
-0.07
-0.08
0.04
0.00
0.03
-0.09
0.12
-0.02
-0.11
-0.01
0.00
-0.02
-0.06
-0.07
0.22
-0.01
-0.09
-0.04
-0.08
0.10
-0.05
-0.17
0.15
-0.03
-0.03
0.06
0.15
0.11
0.02
-0.01
0.17
0.02
0.12
0.01
0.08
0.01
0.06
0.06
0.05
0.07
0.07
0.07
0.06
0.05
0.05
0.04
0.06
0.06
0.06
0.05
0.04
0.05
0.04
0.05
0.09
0.07
0.08
0.06
0.06
0.05
0.05
0.05
0.04
0.05
0.04
0.04
0.03
0.03
0.06
0.05
0.06
0.04
0.04
0.03
0.03
0.06
0.05
0.06
0.05
0.04
0.04
0.04
0.06
0.07
0.05
0.06
0.06
0.04
0.05
0.05
0.06
0.04
1.47
0.05
0.00
0.81
7.03
8.36
9.89
2.35
5.14
0.59
7.94
5.23
0.80
1.04
0.02
1.95
0.23
1.54
43.49
0.07
0.41
0.84
0.26
0.01
0.21
1.38
4.10
0.11
4.16
0.02
0.00
0.24
0.47
1.15
8.61
0.02
2.65
0.83
3.30
1.13
0.48
3.73
4.50
0.26
0.35
1.56
2.86
1.18
0.09
0.03
4.19
0.30
2.64
0.03
0.83
0.01
0.45
0.09
0.04
0.30
-0.99
1.00
1.00
0.74
1.00
0.80
1.00
1.00
-0.41
0.56
-0.08
-0.88
0.63
-0.63
0.99
-0.06
-0.13
-0.21
0.15
0.93
0.24
-0.38
0.76
-0.12
-0.81
-0.08
-0.02
-0.45
-0.20
-0.36
0.93
-0.05
-0.70
-1.00
-1.00
0.27
-0.22
-0.54
0.67
-0.21
-1.00
0.89
0.57
0.33
0.14
-0.06
0.72
1.00
0.63
0.08
0.36
0.06
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
1
1
1
1
1
1
1
1
1
1
1
1
1
2
2
2
2
2
2
2
3
3
3
3
3
3
3
0
0
0
0
0
0
0
1
1
1
1
1
1
1
2
2
2
2
2
2
2
3
3
3
3
3
3
3
0
0
0
0
0
0
0
1
1
1
3
4
5
6
0
1
2
3
4
5
6
0
1
2
3
4
5
6
0
1
2
3
4
5
6
0
1
2
3
4
5
6
0
1
2
3
4
5
6
0
1
2
3
4
5
6
0
1
2
3
4
5
6
0
1
2
0.04
0.05
-0.03
0.03
-0.05
0.00
0.04
0.10
0.01
-0.01
0.04
0.12
-0.14
-0.06
-0.02
-0.17
0.00
-0.15
0.56
-0.01
-0.03
0.00
-0.10
0.01
0.07
-0.06
0.03
0.08
-0.03
0.02
0.01
-0.11
-0.11
0.07
0.15
-0.02
0.02
0.01
-0.06
-0.08
-0.06
-0.22
0.00
-0.08
-0.01
0.06
0.41
-0.07
0.03
0.02
-0.08
-0.09
-0.06
0.10
0.15
0.05
0.05
0.05
0.03
0.05
0.06
0.06
0.04
0.05
0.05
0.04
0.05
0.07
0.07
0.05
0.06
0.06
0.04
0.06
0.09
0.06
0.07
0.05
0.05
0.05
0.06
0.05
0.04
0.05
0.04
0.03
0.03
0.04
0.06
0.05
0.06
0.04
0.04
0.04
0.05
0.07
0.05
0.06
0.05
0.04
0.04
0.05
0.10
0.06
0.08
0.06
0.05
0.05
0.06
0.07
0.05
0.06
0.26
0.38
0.68
0.20
0.31
0.00
0.29
1.78
0.01
0.11
0.39
1.41
1.83
0.64
0.04
3.60
0.00
3.92
55.93
0.01
0.08
0.00
2.23
0.05
0.77
0.66
0.32
1.24
0.26
0.13
0.05
3.47
1.88
1.67
3.88
0.12
0.19
0.02
0.85
0.73
0.99
7.60
0.01
2.06
0.06
0.74
13.25
0.63
0.07
0.03
1.17
2.06
0.64
1.04
5.86
0.37
0.24
0.27
-1.00
0.22
-0.27
0.00
0.46
0.73
0.05
-1.00
0.38
0.40
-0.42
-0.41
-0.08
-0.69
-1.00
-0.83
1.00
-0.02
-0.06
-0.01
-0.85
0.85
0.24
-0.26
0.23
0.34
-0.28
0.35
0.66
-0.72
-0.31
0.39
0.47
-0.14
0.32
0.28
-0.29
-0.18
-0.28
-0.59
0.03
-0.88
-0.68
0.25
0.85
-0.30
0.08
0.08
-0.94
-1.00
-0.38
0.35
1.00
0.23
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
1
1
1
1
1
1
1
1
1
1
1
1
1
2
2
2
2
2
2
2
3
3
3
3
3
3
3
0
0
0
0
0
0
0
1
1
1
1
1
1
1
2
2
2
2
2
2
2
3
3
3
3
3
3
3
0
0
0
0
0
0
0
1
1
1
3
4
5
6
0
1
2
3
4
5
6
0
1
2
3
4
5
6
0
1
2
3
4
5
6
0
1
2
3
4
5
6
0
1
2
3
4
5
6
0
1
2
3
4
5
6
0
1
2
3
4
5
6
0
1
2
-0.05
0.07
0.05
0.03
0.10
-0.08
0.06
0.03
0.03
-0.04
0.02
0.11
-0.05
-0.08
0.00
0.09
0.03
0.06
0.56
-0.01
-0.11
-0.02
0.06
-0.07
0.05
0.13
0.16
-0.03
-0.13
0.18
0.04
0.07
0.12
-0.06
0.10
0.06
-0.09
-0.07
0.00
0.04
-0.10
-0.04
0.00
-0.17
-0.05
-0.02
0.48
-0.11
0.01
0.01
0.01
-0.04
0.00
-0.18
0.24
0.13
0.05
0.04
0.04
0.04
0.06
0.05
0.06
0.04
0.04
0.04
0.04
0.09
0.07
0.08
0.06
0.05
0.05
0.06
0.09
0.06
0.07
0.05
0.07
0.05
0.04
0.06
0.05
0.05
0.04
0.05
0.04
0.03
0.06
0.04
0.05
0.04
0.05
0.04
0.03
0.06
0.04
0.05
0.04
0.05
0.04
0.03
0.10
0.07
0.06
0.06
0.05
0.05
0.05
0.08
0.07
0.06
0.71
2.60
1.10
0.24
1.01
1.05
0.66
0.29
0.22
0.65
0.07
0.78
0.31
0.61
0.00
1.69
0.21
0.66
38.98
0.02
1.53
0.07
0.37
0.90
0.79
2.35
7.39
0.15
5.08
6.58
0.51
2.72
2.13
1.00
2.32
1.27
1.31
2.03
0.00
0.20
2.91
0.31
0.00
5.64
0.81
0.20
18.17
1.36
0.01
0.02
0.02
0.39
0.00
2.55
9.14
2.79
-0.40
1.00
1.00
0.31
0.59
-0.67
0.45
0.42
0.78
-1.00
0.27
0.23
-0.19
0.22
0.00
1.00
1.00
0.41
1.00
-0.03
-0.24
-0.08
0.13
-0.34
0.46
0.36
0.77
-0.10
-0.79
0.72
0.32
1.00
0.48
-0.38
0.44
0.54
-0.38
-0.84
0.03
0.11
-0.56
-0.15
0.02
-0.69
-0.42
-0.33
0.99
-0.34
0.04
0.05
0.07
-0.36
0.00
-0.51
0.84
0.57
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
2
2
2
2
2
2
2
3
3
3
3
3
3
3
3
4
5
6
0
1
2
3
4
5
6
0
1
2
3
4
5
6
-0.04
0.05
0.08
0.05
-0.24
0.17
0.05
-0.09
0.02
0.03
-0.01
-0.02
-0.08
0.01
0.04
-0.03
0.01
-0.01
0.05
0.05
0.05
0.05
0.08
0.07
0.06
0.05
0.05
0.04
0.05
0.07
0.07
0.06
0.05
0.05
0.04
0.05
0.30
0.66
1.82
0.70
3.83
4.19
0.39
1.58
0.09
0.31
0.02
0.02
0.99
0.01
0.31
0.19
0.03
0.04
-0.20
0.36
0.64
0.36
-0.64
0.64
0.26
-0.59
0.15
0.35
-0.07
-0.06
-0.29
0.05
0.23
-0.21
0.10
-0.09
Supplementary Online Table S5. Pearson product moment correlation coefficients, calculated
among dam (full-sib) families and among sire (half-sib) families, between female pulse-pair rate
(PR) threshold and male life history and song characters (pulse-pair rate, PR; peak amplitude,
PA; asynchrony interval, AI; attractiveness index, AT). Calculations were made on transformed
data (see text).
Maryland population
High temperature
Female PR threshold – Male Development period
Low temperature
High de
Full-sib
Half-sib
Full-sib
Half-sib
Full-sib
0.156
0.198
-0.092
-0.025
-0.107
-0.107
-0.212
-0.061
-0.022
-0.103
-0.332**
-0.442*
-0.195
-0.136
-0.246*
PR threshold -
Body weight
PR threshold -
Adult longevity
PR threshold -
PR
-0.026
0.072
-0.03
-0.011
0.297*
PR threshold -
PA
-0.289*
-0.284
-0.075
-0.16
-0.019
PR threshold -
AI
-0.002
-0.13
-0.001
0.135
0.096
PR threshold -
AT
-0.237*
-0.306
-0.068
-0.128
0.03
Louisiana population
High temperature
Female PR threshold – Male Development period
Low temperature
High de
Full-sib
Half-sib
Full-sib
Half-sib
Full-sib
-0.012
0.111
-0.319*
-0.419
-0.228
PR threshold -
Body weight
-0.039
0.094
-0.216
-0.043
-0.215
PR threshold -
Adult longevity
-0.093
0.041
0.096
0.161
-0.052
PR threshold -
PR
-0.026
-0.138
-0.024
-0.02
0.012
PR threshold -
PA
0.091
0.249
-0.25
-0.345
-0.264
PR threshold -
AI
0.116
0.319
0.097
0.132
-0.08
PR threshold -
AT
0.125
0.209
-0.208
-0.244
-0.221
*, **, *** : p < 0.05, 0.01, 0.001, respectively. boldface : p < .05 following
Holm correction for multiple tests.