Supplementary Table 4: NNK versus ethanol effects on protein/lipid adducts in liver
Protein
Ethanol Factor
NNK Factor
Ethanol x NNK
Interaction
Competitive ELISA
F-Ratio
P-value
F-Ratio
P-Value
F-Ratio
P-Value
Keratin-18
2.114
0.149
99.73
<0.0001
1.160
N.S.
Protein Carbonyl
0.785
N.S.
0.761
N.S.
0.896
N.S.
HNE
8.929
0.006
2.469
0.127
2.330
0.138
8-iso-PGF2
5.081
0.032
2.101
0.158
0.4576
N.S.
Supplementary Table 4: Immunoreactivity was measured by competitive ELISAs. Results
were analyzed by 2-way ANOVA and the Fisher multiple comparisons post-test. F-ratios and
P-values reflecting variance with respect to (Control vs Ethanol), Vehicle-VEH vs NNK
treatment, and interactions between ethanol and NNK are indicated. Abbreviations and
symbols: HNE=4-hydroxynonenal, 8-iso-PGF2α= 8-epimer of Prostaglandin F2α. Livers were
harvested approximately 30 minutes after an ethanol or saline binge.