Application of ecogenomics to the assessment of the ecological
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50 45 Proportion of total OTUs 40 35 30 Triclosan Control 25 Triclosan Low 20 Triclosan High 15 10 5 0 1 2 3 4 Number of replicates 5 6 Supplementary Figure 1. The distribution of occurrences (presence/absence observations) for OTUs sampled and sequenced from the surficial sediments of the three triclosan modified treatments. 1 Supplementary Table 1. Summary BLAST and taxonomic allocation results of the two sequenced reference samples obtained from Fundulus heteroclitus. Nearest taxonomic level is lowest common taxonomic level between the highest BLAST hit and Fundulus heteroclitus. Final corrected taxonomic assignment refers to the final assignment given to the OTU subsequent to phylogenetic tree visualization and Linnaeus Blasts. uk = unknown. OTU name Sum of reads 7240 35020 32366 29353 4290 19259 11246 34736 9923 19934 50573 52816 690 511 435 373 333 331 276 256 Corrected similarity (%) 99 79 74 97 68 89 94 68 94 95 26518 224 5625 6446 9920 4104 28281 28268 34083 30036 26823 25568 29335 10308 753 32590 207 176 145 130 124 96 95 78 64 60 58 54 53 51 Bit-Score E Value Accession Description 694 252 623 337 206 646 601 206 553 455 0 6.49E-64 3.52E-175 2.09E-89 6.90E-50 0 1.42E-168 6.90E-50 3.67E-154 8.34E-125 M91180 DQ093794 M91180 M91180 EF431912 M91180 M91180 EF431912 M91180 M91180 Fundulus heteroclitus Pterospora floridiensis Fundulus heteroclitus Fundulus heteroclitus Cyprinodon variegatus Fundulus heteroclitus Fundulus heteroclitus Cyprinodon variegatus Fundulus heteroclitus Fundulus heteroclitus 96 494 1.87E-136 M91180 97 95 78 97 94 95 93 70 96 97 72 72 91 57 297 603 502 376 291 374 439 461 524 365 261 261 359 470 3.02E-77 3.81E-169 1.40E-138 4.85E-101 1.53E-75 1.95E-100 8.49E-120 2.21E-126 2.52E-145 1.04E-97 1.45E-66 1.45E-66 5.76E-96 4.91E-129 HQ615532 M91180 M91180 M91180 CR696643 M91180 M91180 M91180 M91180 M91180 EF431912 EF431912 M91180 M91180 Fundulus heteroclitus Craterocephalus stercusmuscarum Fundulus heteroclitus Fundulus heteroclitus Fundulus heteroclitus Tetraodon nigroviridis Fundulus heteroclitus Fundulus heteroclitus Fundulus heteroclitus Fundulus heteroclitus Fundulus heteroclitus Cyprinodon variegatus Cyprinodon variegatus Fundulus heteroclitus Fundulus heteroclitus 2 Nearest correct taxonomic level Final, corrected taxonomic assignment Species Superkingdom Species Species Order Species Species Order Species Species Species Family uk eukaryote uk eukaryote Family uk eukaryote Kingdom Class uk eukaryote Class Order Class Species Species Species Class Species Species Species Species Species Order Order Species Species Family Order uk eukaryote Family Class Order Class uk eukaryote Order Family uk eukaryote uk eukaryote Phylum uk eukaryote Order Supplementary Table 1 (continued) OTU name Sum of reads 950 20361 6109 42 42 39 Corrected similarity (%) 96 93 96 27400 39 2190 5754 37217 29596 4740 3588 25985 14798 9258 6241 9605 14975 993 Bit-Score E Value Accession Description 498 237 295 1.47E-137 1.74E-59 1.13E-76 M91180 CR696643 M91180 36 99 9.07E-18 JQ896300 36 28 28 25 25 23 36 96 87 95 95 94 99 588 407 306 450 502 9.07E-18 9.77E-165 3.05E-110 5.41E-80 3.85E-123 1.20E-138 JQ896300 M91180 FJ790717 EF431912 M91180 M91180 22 21 20 20 20 19 18 91 52 96 95 93 91 49 236 430 286 442 420 644 400 7.07E-59 8.38E-117 6.44E-74 6.30E-121 2.95E-114 0 6.52E-108 HQ615532 M91180 M91180 M91180 M91180 M91180 M91180 Fundulus heteroclitus Tetraodon nigroviridis Fundulus heteroclitus Hypophthalmichthys molitrix Hypophthalmichthys molitrix Fundulus heteroclitus Cavernomonas stercoris Cyprinodon variegatus Fundulus heteroclitus Fundulus heteroclitus Craterocephalus stercusmuscarum Fundulus heteroclitus Fundulus heteroclitus Fundulus heteroclitus Fundulus heteroclitus Fundulus heteroclitus Fundulus heteroclitus 3 Nearest correct taxonomic level Final, corrected taxonomic assignment Species Class Species Order Class Order Order uk eukaryote Order Species Superkingdom Order Species Species uk eukaryote Order uk eukaryote Order Order Class Class Species Species Species Species Species Species Phylum uk eukaryote Order Order Class Phylum uk eukaryote
