Supplementary table Eighty SSR markers used to detect homozygous SSR loci ratio of wheat varieties Locus Marker Chromoso type me* Xcwm6 EST5 SSR 1A Numb PIC er of value alleles Fragment size Marker grade 10 0.66 220-260 1 (bp) Xbarc8 0 Genom 1B ic-SSR 6 0.61 97-125 1 Xcfd72 Genom 1D ic-SSR 11 0.66 210-240 1 Xgwm2 Genom 2A 94 ic-SSR 16 0.74 67-105 1 Xgwm4 Genom 2B 29 ic-SSR 12 0.67 190-230 1 Xgwm2 Genom 2D 61 ic-SSR 11 0.67 160-210 1 Xgwm1 Genom 3A/1D 55 ic-SSR 9 0.74 120-160 1 Xswes1 EST85 SSR 4 0.58 180-190 1 10 0.71 120-150 1 Xgdm7 2 3B Genom 3D ic-SSR Xgwm6 Genom 4A 10 ic-SSR 7 0.55 160-175 1 Xksum6 EST2 SSR 9 0.77 170-210 1 Genom 2B/4D ic-SSR 12 0.76 110-160 1 Xgwm3 Genom 2A/5A 04 ic-SSR 16 0.80 200-230 1 Xgwm6 Genom 3A/5B/7D 7 ic-SSR 8 0.58 70-100 1 Xcfd29 Genom 5D ic-SSR 14 0.82 160-190 1 Xgwm3 Genom 6A 34 ic-SSR 21 0.80 100-140 1 Xbarc1 98 Genom 6A /6B ic-SSR 12 0.66 110-160 1 Xcfd76 Genom 6D ic-SSR 14 0.79 140-170 1 Xcfa20 28 Genom 7A/7B ic-SSR 7 0.49 230-260 1 Xgwm3 Genom 7B 33 ic-SSR 8 0.61 140-150 1 Xgwm4 Genom 7D 18 0.69 90-140 1 Xbarc9 1 4B 37 ic-SSR Xbarc1 7 Genom 1A ic-SSR 8 0.77 250-280 2 4 0.50 210-240 2 Genom 1A/1B/1D/ 8 ic-SSR 5B 0.70 240-280 2 Xcwm7 EST5 SSR Xbarc2 40 1A Xgwm2 Genom 1B 68 ic-SSR 19 0.82 230-250 2 Xcfd48 5 0.79 250-380 2 Genom 1B ic-SSR Xcwm1 EST70 SSR 1D 2 0.37 230-250 2 Xcwm3 EST34 SSR 2A 3 0.53 175-180 2 Xgwm4 Genom 2A 25 ic-SSR 9 0.72 120-141 2 Xwmc7 Genom 2B 64 ic-SSR 7 0.81 130-180 2 Xgwm5 Genom 2D 39 ic-SSR 13 0.83 130-170 2 Xksum7 EST3 SSR 3 0.55 160-180 2 2D Xwmc5 Genom 3A 32 ic-SSR 8 0.59 160-200 2 Xbarc3 24 Genom 3A ic-SSR 7 0.69 247-380 2 Xwmc3 Genom 3B 26 ic-SSR 8 0.76 170-210 2 Xgwm1 Genom 3D 61 ic-SSR 8 0.69 150-170 2 Xwmc4 Genom 4A 68 ic-SSR 12 0.84 130-170 2 Xgwm4 Genom 4B 95 ic-SSR 9 0.75 150-180 2 Xwmc4 Genom 4D 57 ic-SSR 4 0.58 160-170 2 Xcfa21 55 Genom 5A ic-SSR 5 0.74 220-240 2 Xbarc2 1 Genom 5B/6D ic-SSR 7 0.65 200-215 2 5B 2 0.36 160-200 2 Genom 5D ic-SSR 7 0.66 150-160 2 Xgwm1 Genom 6A 8 0.70 180-235 2 Xcwm2 EST32 SSR Xcfd8 69 ic-SSR Xgwm5 Genom 6A/6B 70 ic-SSR 8 0.74 120-150 2 Xbarc3 54 Genom 6B ic-SSR 7 0.75 180-260 2 Xgwm3 Genom 6D 25 ic-SSR 8 0.76 130-150 2 Xcfd49 Genom 6D ic-SSR 12 0.82 160-240 2 Xwmc6 Genom 7A 03 ic-SSR 10 0.74 90-120 2 Xwmc7 Genom 7D 02 ic-SSR 8 0.80 150-190 2 Xksum1 Genom 1A 82 ic-SSR 4 0.53 170-200 3 Xcnl105 ESTSSR 1B 2 0.37 110-140 3 Xbarc1 74 Genom 1B ic-SSR 7 0.76 160-230 3 Xgdm1 11 Genom 1D ic-SSR 5 0.61 280-320 3 Xbarc1 68 Genom 2D ic-SSR 4 0.56 150-180 3 Xwmc2 Genom 3A 64 ic-SSR 11 0.82 120-150 3 Xwmc4 Genom 3A 28 ic-SSR 8 0.72 230-260 3 Xbarc1 2 Genom 3A ic-SSR 9 0.84 201-350 3 Xgwm5 Genom 3B 66 ic-SSR 7 0.76 120-140 3 Xwmc7 Genom 3B 77 ic-SSR 11 0.67 100-170 3 Xcfd15 2 7 0.73 290-350 3 Xgwm6 Genom 3D 45 ic-SSR 12 0.89 135-170 3 Xksum1 EST34 SSR 2 0.36 250-280 3 Genom 3D ic-SSR 4A Xbarc1 70 Genom 4AL ic-SSR 13 0.80 110-150 3 Xbarc2 0 Genom 4B/7B ic-SSR 6 0.74 180-210 3 Xwmc3 Genom 4B 49 ic-SSR 7 0.57 85-110 3 Xwmc2 Genom 4D 3 0.62 290-320 3 85 ic-SSR Xwmc7 Genom 4D 20 ic-SSR 13 0.81 100-140 3 Xgwm1 Genom 5A 86 ic-SSR 7 0.75 132-180 3 Xcwem 44 5A/5B/5D 4 0.74 50-70 3 5B 2 0.38 160-175 3 Xgwm6 Genom 5A/6A 17 ic-SSR 11 0.76 100-180 3 Xwmc1 Genom 3B/6A ic-SSR 5 0.47 130-160 3 Xgwm4 Genom 6A 59 ic-SSR 13 0.82 118-126 3 Xbarc1 96 Genom 6D ic-SSR 3 0.42 130-217 3 Xcfd33 Genom 6D ic-SSR 3 0.51 170-242 3 Xgwm4 Genom 6D 69 ic-SSR 9 0.80 150-180 3 Xswes2 EST09 SSR 4 0.63 280-300 3 ESTSSR Xwes26 ESTSSR 7B Xgwm3 Genom 7B 44 ic-SSR 8 0.61 130-150 3 Xgwm4 Genom 7D 4 ic-SSR 12 0.84 160-210 3