Supplementary Table I. Correlations of measured variables in the present study and the equation describing each relationship (if the P-value was <0.05), r2, and P-values. Site Independent Dependant Equation r2 P-value CFR 1 Length C:N C:N = 0.63L + 0.56 0.29 0.049* CFR 2 Length C:N C:N = 0.14L + 3.8 0.35 0.008* MR 1 Length C:N NA 0.05 0.37 MR 2 Length C:N C:N = 0.27L + 3.1 0.24 0.028* CFR 1 Length δ13C δ13C = 0.53L – 30.5 0.35 0.026* CFR 2 Length δ13C δ13C = 0.36L – 27.9 0.48 0.001* MR 1 Length δ13C NA 0.03 0.47 MR 2 Length δ13C δ13C = 0.25L – 28.8 0.26 0.020* CFR 1 Length δ15N NA 0.007 0.77 CFR 2 Length δ15N NA 0.004 0.79 MR 1 Length δ15N NA 0.0006 0.92 MR 2 Length δ15N NA 0.06 0.32 CFR 1 C:N δ13C δ13C = 0.58[C:N] – 29.0 0.57 0.002* CFR 2 C:N δ13C δ13C = 1.1[C:N] – 30.7 0.24 0.034* MR 1 C:N δ13C δ13C = 0.84[C:N] – 32.5 0.59 <0.0001* MR 2 C:N δ13C δ13C = 0.70[C:N] – 30.1 0.62 <0.0001* CFR 1 C:N δ15N NA 0.006 0.80 CFR 2 C:N δ15N NA 0.14 0.11 MR 1 C:N δ15N δ15N = -0.16[C:N] + 8.7 0.25 0.030* MR 2 C:N δ15N δ15N = -0.15[C:N] + 8.6 0.29 0.015* MR C:N δ15N δ15N = -0.16[C:N] + 8.6 0.33 0.0001* CFR1 Condition C:N NA 0.009 0.74 CFR2 Condition C:N NA 0.001 0.90 MR1 Condition C:N NA 0.17 0.11 MR2 Condition C:N NA 0.03 0.44 MR Condition C:N K = 0.040[C:N] + 1.4 0.13 0.025* *, α ≤ 0.05 NA = Not applicable CFR = Clear Fork River MR = Mad River Supplementary Figure 1. Ammocoete condition factor (K) vs. C:N ratio for (a) the Clear Fork River and (b) the Mad River study sites. Black circles are Clear Fork River 1, gray circles are Clear Fork River 2, black squares are Mad River 1, and gray squares are Mad River 2. The correlation indicate for the Mad River is for both sites combined.