Table S1. Posttranslational modifications reported for plant aquaporins.
Modification
Phosphorylation
Glycosylation
Methylation
Acetylation
Aquaporin
GmNOD26
SoPIP2;1/PM28A
Plant
Soybean
Spinach
Protein Acc. no
XP_003524979
AAA99274
AGV54386
Modified residue position
Ser262
Ser115 and Ser274
Ser188
Ser7, Ser23 and Ser99
Regulation
Gating
Gating
Gating
Gating
Reference
Guenther et al. 2003
Johansson et al. 1998
Nyblom et al. 2009
Maurel et al. 1995
PvTIP3;1/
PvTIPAGV54386.1
LjLIMP2
Phaseolus
Lotus
AAF82791
Ser265
Gating
Guenther and Roberts 2000
MpPIP1;1
Mimosa
pudica
BAD90696
Ser131
Temmei et al. 2005
TgPIP2;2
AtPIP2;1
McTIP1;2/MIP-F
AtPIP2;1,
AtPIP2;2
AtPIP1;1
AtPIP1;2
AtPIP1;3
AtPIP1;4
Tulip
Arabidopsis
Ice plant
Arabidopsis
BAG68662
NP_190910
AAB17284
NP_190910,
NP_181254
CAB71073
AAC28529
AAF81320
AAF02782
Ser35, Ser116 and Ser274
Ser283
Asn80, Asn97
Lys3 (di-methylated) and Glu6
(mono-methylated)
Met1
Heterodimer (
MpPIP1;1MpPIP2;1)
gating
Gating
Trafficking
Trafficking
-
Santoni et al. 2006
Arabidopsis
Azad et al. 2008
Prak et al. 2008
Vera-Estrella et al. 2004
Santoni et al. 2006
References
Azad AK, Katsuhara M, Sawa Y, Ishikawa T, Shibata H (2008) Characterization of four plasma membrane aquaporins in tulip petals: a
putative homolog is regulated by phosphorylation. Plant Cell Physiol 49: 1196–1208
Guenther JF, Roberts DM (2000) Water-selective and multifunctional aquaporins from Lotus japonicus nodules. Planta 210:741–748
Guenther JF, Chanmanivone N, Galetovic MP, Wallace IS, Cobb JA, Roberts DM (2003) Phosphorylation of soybean nodulin 26 on serine
262 enhances water permeability and is regulated developmentally and by osmotic signals. Plant Cell 15:981–991
Johansson I, Karlsson M, Shukla VK, Chrispeels MJ, Larsson C, Kjellbom P (1998) Water transport activity of the plasma membrane
aquaporin PM28A is regulated by phosphorylation. Plant Cell 10:451–459
Maurel C, Kado RT, Guern J, Chrispeels MJ (1995) Phosphorylation regulates the water channel activity of the seed-specific aquaporin -TIP.
EMBO J 14:3028–3035
Nyblom M, Frick A, Wang Y, Ekvall M, Hallgren K, Hedfalk K, Neutze R, Tajkhorshid E, Törnroth-Horsefield S (2009) Structural and
functional analysis of SoPIP2;1 mutants adds insight into plant aquaporin gating. J Mol Biol 387(3)653–668
Prak S, Hem S, Boudet J, Viennois G, Sommerer N, Rossignol M, Maurel C, Santoni V (2008) Multiplephosphorylations in the C-terminal
tail of plant plasma membrane aquaporins. Mol Cell Proteomics 7:1019-1030
Santoni V, Verdoucq L, Sommerer N, Vinh J, Pflieger D, Maurel C (2006) Methylation of aquaporins in plant plasma membrane. Biochem J
400:189–197
Temmei Y, Uchida S, Hoshino D, Kanzawa N, Kuwahara M, Sasaki S, Tsuchiya T (2005) Water channel activities of Mimosa pudica plasma
membrane intrinsic proteins are regulated by direct interaction and phosphorylation. FEBS Lett 579: 4417–4422
Vera-Estrella R, Barkla BJ, Bohnert HJ, Pantoja O (2004) Novel regulation of aquaporins during osmotic stress. Plant Physiol 135:2318–2329