Prof Yair Aharanowitz’s Publications
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Aharonowitz, Y., and G. Cohen, (1981) The microbial production of
pharmaceuticals. Scientific American, Sept. 140-152.
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Shiffman,D., Cohen,G., Aharonowitz,Y.,Palissa,H., von Dohren,H., Kleinkauf, H.,
and Mevarech,M., (1990) Nucleotide sequence of the Isopenicillin N synthase
gene (pcbC) of the gram-negative Flavobacterium sp. SC 12,154. Nucleic Acid
Research 18: 660.
Medlib
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Cohen, G., Shiffman, D., Mevarech, M., and Aharonowitz,Y., (1990) The
microbial Isopenicillin N synthase genes: structure, function, diversity and
evolution. Trends in Biotechnology, 8:105-111.
Medlib
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Abramov,S., Aharonowitz,Y., Harnik,M., Lamed,R., and Freeman,A., (1990)
Continuous stereospecific delta 4-3-Keto-steroid reduction by PAAH bead
entrapped Clostridium paraputrificum cells. Enz. Microb. Technology, 12: 982988.
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Landan,G., Cohen,G., Aharonowitz,Y., Shauli,Y., Graur,D., and Shiffman,D.,
(1990) Evolution of isopenicillin N synthase genes may have involved horizontal
gene transfer. Molec. Biol. Evol. 7: 399-406
Medlib
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Landman,O., Shiffman,D., Av-Gay,Y., Aharonowitz,Y., and Cohen,G., (1991).
High level expression inEscherichia coli of isopenicillin N synthase genes
from Flavobacterium and Streptomyces and recovery of active enzyme from
inclusion bodies. FEMS Microbiol. Lett. 84:239-244
Medlib
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Schwecke,T., Aharonowitz,Y., Palissa,H., von Dohren,H., Kleinkauf, H., van
Liempt,H., (1992). Enzymatic characterization of the multifunctional enzyme d-(La-aminoadipyl)-L-cysteinyl-D-valine synthetase fromStreptomyces
clavuligerus. Eur. J. Biochem 205:687-694
Medlib
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Av-Gay,Y., Aharonowitz,Y., Cohen,G., (1992). Streptomyces contain a 7.0 kDa
cold-shock protein. Nucleic Acid Research. 20:5478
Medlib
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Aharonowitz, Y., Cohen,G., and Martin J.F., (1992), Penicillin and cephalosporin
biosynthetic genes: structure, organization, regulation and evolution. Annu. Rev.
Microbiol. 46: 461-495
Medlib
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Aharonowitz, Y., Av-Gay,Y., Schreiber, Cohen G., (1993) Characterization of a
thioredoxin-like disulfide reductase from Streptomyces clavuligerus and its
possible role in b-lactam antibiotic biosynthesis. J.Bacteriol. 175: 623-629
Medlib
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Newton, J., Fahey, R.C., Cohen,G., and Aharonowitz, Y., (1993) Low molecular
weight thiols in Streptomycetes and their potential role as antioxidants. J
.Bacteriol. 175:2734-2742
Medlib
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Aharonowitz,Y., Bergmeyer,H., Cantoral, J., Cohen,G., Demain, A.L., Fink,U.,
Kinghorn, J., H. Kleinkauf, MacCabe,A., Palissa,H., Pfeifer,E.,Schwecke,T., van
Limpt,H., von Dohren,H., Wolfe,S., and Zhang,J.,(1993). d-(L-a-aminoadipyl)-Lcysteinyl-D-valine synthetase, themultienzyme integrating the four primary
reactions in b-lactam biosynthesis, as a model peptide synthetase.
Bio/technology 11:807-810.
Medlib
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Cohen, G., Janko, M., Mislovati,R., Argaman, A., Schreiber, R., Av-Gay, Y., and
Aharonowitz, Y., (1993) Thioredoxin-thioredoxin reductase system of
Streptomyces clavuligerus: sequence, expression and organization of the genes.
J.Bacteriol. 175:5159-5167
Medlib
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Cohen,G., Argaman, A., Schreiber, R., Mislovati, M., and Aharonowitz, Y., (1994)
The thioredoxin system ofStreptomyces clavuligerusand its possible role in
penicillin biosynthesis. J. Bacteriol. 176: 973-984
Medlib
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Newton, G. L., Bewley, G. A., Dwyer, T. J., Horn, R., Aharonowitz, Y., Cohen, G.,
Davies, J. E., Faulkner, D. J., and Fahey, R. C., (1995) The structure of U17
isolated from Streptomyces clavuligerusand its properties as an antioxidant thiol.
Eur. J. Biochem 230:821-825.
Medlib
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Cohen, G., and Aharonowitz, Y., (1995) Molecular genetics of antimicrobials: a
case study of beta-lactam antibiotics. Darby, G.K., Hunter, P.A., Russel, A. D.,
eds. 50 Years of Microbials, Soc. Gen. Microbiol, Symp. 53. 139-163. Cambridge
University Press. UK.
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Borovok. I., Landman, O., Kreisberg, R., Aharonowitz, Y., and Cohen, G., (1996).
The ferrous active site of isopenicillin N synthase: genetic and sequence analysis
of the endogenous ligands. Biochemistry, 35:1981-1987
Medlib
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Newton, G., Arnold, K., Price, M. S., Sherrill, C., Delcardayre, S. B., Aharonowitz,
Y., Cohen, G., Davies, J. E., and Fahey, R. C., and Davis, C., (1996) Distribution
of thiols in microorganisms: mycothiol is a major thiol in most actinomycetes. J.
Bacteriol. 178:1990-1995
Medlib
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Gal-Mor, O., Borovok, I., Av-Gay, Y., Cohen, G., And Aharonowitz, Y., (1998).
Gene organization in the trxA/B -oriC region of the Streptomyces
coelicolor chromosome and comparison with other bacteria. Gene, 217: 83-90
Medlib
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Kreisberg-Zakarin, R., Brorovok, I., Yanko, M., Aharonowitz, Y., and Cohen G.,
(1999). Recent advances in the structure and Function of Isopenicillin N
synthase. Antonie van Leeuwenhoek 75: 33-39
Medlib
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Kreisberg-Zakarin, R., Brorovok, I., Yanko, M., Frolow, F., Aharonowitz, Y., and
Cohen, G., 2000. Structure-function studiesn of the non-heme iron active site of
isopenicillin N synthase: some implications for catalysis. Biophysical Chemistry.
86: 109-118
Medlib
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Masalha, M., Borovok, I., Schreiber, R., Aharonowitz Y., and Cohen., G., (2001)
Analysis of Transcription of theStaphylococcus aureus Aerobic Class Ib and
Anaerobic Class III Ribonucleotide Reductase Genes in Response to Oxygen. J
Bacteriol. 183: 7260-7272
PDF (1MB)
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Paget, M. S. B., Molle, M., Cohen, G., Aharonowitz, Y.,and Buttner M. J.,(2001)
Defining the disulphide stress response in Streptomyces coelicolor A3(2):
identification of the sR regulon. Molecular Microbiology. 42: 1007-1020
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Ilya Borovok , Rachel Kreisberg-Zakarin1, Michaela Yanko, Rachel Schreiber,
Margarita Myslovati, Fredrik Aslund, Arne Holmgren, Gerald Cohen and Yair
Aharonowitz (2002) Streptomyces contain class Ia and class II ribonucleotide
reductases expression analysis of the genes in vegetative growth. Microbiology.
148: 391-404
PDF (.85MB)
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Dafna Ben-Bashat, Yael Meller, Yair Aharonowitz, David utnick, Shmuel Carmeli,
and Gil Navon. (2002) Excretion of a Phosphorus-Containing Carbohydrate
by Streptomyces sp. A50. J. Natural Products. 64: 1538-1540
PDF (.05MB)
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Uziel Orit, Ilya Borovok, Rachel Schreiber, Gerald Cohen and Yair Aharonowitz.
(2004) Transcriptional regulation of the Staphylococcus aureus thioredoxin and
thioredoxin reductase genes in responde to oxygenand disulfide stress. Journal
of Bacteriology 186: 326-334
PDF (2.25MB)
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James K. Lithgow, Emma J. Hayhurst, Gerald Cohen, Yair haronowitz, and
Simon J. Foster (2004) Role of a Cysteine Synthase in Staphylococcus aureus.
J. Bacteriol. 186: 1579-1590
PDF (0.25MB)
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Borovok, I., Gorovitz, B., Yanku, M., Schreiber, R., Gust, B., Chater, K.,
Aharonowitz, Y., Cohen, G., (2004) Alternative oxygen-dependent and oxygenindependent ribonucleotide reductases in Streptomyces: cross-regulation and
physiological role in response to oxygen limitation. Molecular Microbiology
54:1022-1035.
PDF (0.3MB)
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Gopal, S., Borovok, I., Ofer, A., Yanku, M., Cohen, G., Goebel, W., Kreft, J.,and
Aharonowitz, Y., (2005). A multidomain fusion protein in Listeria monocytogenes
catalyzes the two primary activities for glutathione biosynthesis. J. Bacteriol. 187:
3839-3847
PDF (0.7MB)
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Borovok, I., Gorovitz, B., Schreiber, R., Aharonowitz, Y., Cohen, G., (2006)
Coenzyme B12 Controls Transcription of the Streptomyces Class Ia
Ribonucleotide Reductase nrdABS Operon via a Riboswitch Mechanism. J.
Bacteriol. 188: 2512-2520
PDF (0.4MB)
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Zhang, S., Borovok, I., Aharonowitz, Y., Sharan, R., Bafna, V. (2006) A
sequence-based filtering method for ncRNA identification and its application to
searching for riboswitch elements. Bioinformatics. 2006 Jul 15;22(14):e557-65.
PDF (0.2MB)
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Grinberg, I., Shteinberg, T., Gorovitz, B., Aharonowitz, Y., Cohen, G., Borovok, I.
(2006) The Streptomyces coelicolor NrdR protein contains Zn-finger and ATPcone domains and regulates transcription of the ribonucleotide reductase
operons. J. Bacteriol. 188:7635-7644.
PDF (0.6MB)
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Torrents, E. I. Grinberg, B. Gorovitz-Harris, H. Lundström, I. Borovok, Y.
Aharonowitz, B. Sjöberg and Cohen, G. (2007) NrdR controls differential
expression of the Escherichia coli ribonucleotide reductase genes. J Bacteriol.
189:5012-5021.
PDF (0.3MB)
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Makhlin J, Kofman T, Borovok I, Kohler C, Engelmann S, Cohen G, Aharonowitz,
y. (2007) Staphylococcus aureus ArcR controls expression of the arginine
deiminase operon. J Bacteriol. 189:5976-5986.
PDF (0.6MB)
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Grinberg I, Shteinberg T, Hassan Q, Aharonowitz Y, Borovok I, and Cohen, G.
(2009) Functional analysis of the Streptomyces coelicolor NrdR ATP-cone
domain: Role in nucleotide binding, oligomerization and DNA interactions. J.
Bacteriol. 191: 1169-1179.
PDF (1.1MB)
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Malki L, Yanku M, Borovok I, Cohen G, Mevarech M, Aharonowitz, Y. (2009)
Identification and characterization of gshA, a gene encoding the glutamatecysteine ligase in the halophilic archeon Haloferax volcanii. J Bacteriol. 191:
5196-5204
PDF (1.9MB)
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Pöther DC, Liebeke M, Hochgräfe F, Antelmann H, Becher D, Lalk M, Lindequist
U, Borovok I, Cohen G, Aharonowitz Y, Hecker M. (2009) Diamide triggers
mainly S-Thiolations in the cytoplasmic proteomes of Bacillus subtilis and
Staphylococcus aureus. J Bacteriol. 191:7520-7530.
PDF (1.1MB)
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Rabinovitch I, Yanku M, Yeheskel A, Cohen G, Borovok I, Aharonowitz Y. (2010)
Staphylococcus aureus NrdH redoxin is a reductant of the class Ib ribonucleotide
reductase. J Bacteriol. 192:4963-4972.
PDF (3.1MB)
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Ofer A, Kreft J, Logan DT, Cohen G, Borovok I, Aharonowitz Y. (2011)
Implications of the Inability of Listeria monocytogenes EGD-e To Grow
Anaerobically Due to a Deletion in the Class III NrdD Ribonucleotide Reductase
for Its Use as a Model Laboratory Strain. J Bacteriol. 193:2931-2940.
PDF (2.3MB)
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Pöther DC, Gierok P, Harms M, Mostertz J, Hochgräfe F, Antelmann H, Hamilton
CJ, Borovok I, Lalk M, Aharonowitz Y, Hecker M. (2013) Distribution and
infection-related functions of bacillithiol in Staphylococcus aureus. Int J Med
Microbiol. 303:114-23.
PDF (1.0MB)
Patents
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Jensen,S.E., Westlake,D.W.S., Leskiw, B.K., Aharonowitz,Y., and Mevarech,M.,
Cloning and nucleotide sequence determination of the isopenicillin N synthetase
gene from Streptomyces clavuligerus. Application for Canadian Letter Patent,
Aug. 10.1987. serial No 544122
European patent, 88306779.5; 22.07.88
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Aharonowitz,Y. et.al. ACV reductase system, the set of ACV reductase genes
and a method for influencing and using the expression of these genes for
increasing antibiotic production.
European Patent Application EP 0-462-674-A1 Date of filling 18.06.91
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Aharonowitz, Y., et al. 1994. Oxido reductase enzyme system obtained from P.
chrysogenum. Oxido reductase enzyme system obtainable from P.
chrysogenum, the set of genes encoding the same and the use of oxido
reductase enzyme systems or genes encoding the same for increasing antibiotic
production
US Patent 5,328,839 July 12 1994.
US Patent 5,652,132 July 29 1997.
US Patent 5,753,435 May 19 1998.
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Aharonowitz, et al., 2004. Recombinant Staphylococcus thioredoxin reductase
and inhibitors thereof useful as antimicrobial agents.
U.S. Patent No. 6,767,536 , July 27, 2004