On surface molecular chemistry using Scanning Probe Microscopy The recent progress in Atomic Force Microscopy (AFM) and Scanning Tunneling Microscopy (STM) with functionalized tips provided unprecedented atomic resolution of single organic molecules [1,2]. Here we will provide insight into origin of the high-resolution AFM/STM images [3,4]. We will show that the highresolution images contain valuable information going beyond simple imaging of chemical structure. We will present a new experimental protocol, which provides straightforward way to resolve the local electrostatic field of a single molecule by means of AFM with functionalized tips with unprecedented resolution. In next, we will demonstrate the possibility to control molecular chirality through the chemical reactions. Namely, we will report on a novel chemical transformation of chirality of individual molecules into the adsorption-induced chirality of flat molecules. This results in the formation of a new type of organic-inorganic chiral surfaces with the globally broken mirror symmetry. The chemical transformation was analyzed at the ultimate single-molecule level utilizing high-resolution AFM/STM microscopy techniques supported by theoretical DFT simulations.  L. Gross et al., Science 325,1110 (2009).  C. Weiss et al., Phys.Rev.Lett. 105, 086103 (2010).  P. Hapala et al., Phys. Rev. B 90, 085421 (2014).  P. Hapala et al., Phys. Rev. Lett. 113, 226101 (2014).