Alterations in lenticular proteins during ageing and selenite-induced
cataractogenesis in Wistar rats
By:Sakthivel, M (Sakthivel, Muniyan)[ 1 ] ; Elanchezhian, R (Elanchezhian, Rajan)[ 1 ] ; Thomas, PA (Thomas, Philip
A.); Geraldine, P (Geraldine, Pitchairaj)[ 1 ]
MOLECULAR VISION
Volume: 16
Issue: 49-50
Pages: 445-453
Published: MAR 16 2010
View Journal Information
Abstract
Purpose: To determine putative alterations in the major lenticular proteins in Wistar rats of different ages and to
compare these alterations with those occurring in rats with selenite-induced cataract.
Methods: Lenticular transparency was determined by morphological examination using slit-lamp biomicroscopy.
Alterations in lenticular protein were determined by sodium dodecyl sulfate-PAGE (SDS-PAGE) and confirmed
immunologically by western blot.
Results: Morphological examination did not reveal observable opacities in the lenses of the rats of different age groups;
however, dense nuclear opacities were noted in lenses of rats in the selenite-cataract group. Western blot assays
revealed age-related changes in soluble and urea-soluble lenticular proteins. Decreased alpha A- and beta B1crystallins in the soluble fraction and aggregation of alpha A-crystallin, in addition to the degraded fragment of beta B1crystallin, in the urea-soluble fraction appeared to occur in relation to increasing age of the rats from which the lenses
were taken; similarly, cytoskeletal proteins appeared to decline with increasing age. The lenses from rats in the selenitecataract group exhibited similar changes, except that there was also high molecular weight aggregation of alpha Acrystallin.
Conclusions: The results of this study suggest that there is loss, as well as aggregation, of alpha A-crystallin in the
aging rat lens, although there is no accompanying loss of lenticular transparency.
Keywords
KeyWords Plus:HUMAN SENILE CATARACT; AGE-RELATED-CHANGES; HUMAN LENS; ALPHACRYSTALLIN; CYTOSKELETAL PROTEINS; MASS-SPECTROMETRY; MOLECULAR CHAPERONE; HEREDITARY
CATARACT; OXIDATIVE STRESS; BETA B1
Author Information
Reprint Address: Geraldine, P (reprint author)
Bharathidasan Univ, Sch Life Sci, Dept Anim Sci, Tiruchchirappalli 620024, Tamil Nadu, India.
Addresses:
[ 1 ] Bharathidasan Univ, Sch Life Sci, Dept Anim Sci, Tiruchchirappalli 620024, Tamil Nadu, India
E-mail Addresses:gerryarchup@yahoo.co.in
Publisher
MOLECULAR VISION, C/O JEFF BOATRIGHT, LAB B, 5500 EMORY EYE CENTER, 1327 CLIFTON RD, N E,
ATLANTA, GA 30322 USA
Categories / Classification
Research Areas:Biochemistry & Molecular Biology; Ophthalmology
Web of Science Categories:Biochemistry & Molecular Biology; Ophthalmology
Document Information
Document Type:Article
Language:English
Accession Number: WOS:000279075100002
PubMed ID: 20300567
ISSN: 1090-0535
Other Information
IDS Number: 614QP
Cited References in Web of Science Core Collection: 47
Times Cited in Web of Science Core Collection: 6