Table 3: Summary of injection combinations of different target sites and concentration of ebony sgRNA (s) and Cas9 mRNA and
information of mutations.
Injected final con.
Combination
(sgRNAs; Cas9
mRNA) (ng/μl)
Injected
Phenotype
Phenotype
(+)
Hatched/L5
eggs
Mutation rate
T7EI (+)
(+)
(%)
Sequenced (+)
Mutation types
(%)
T2
40; 300
1857
547/351
0 (351)
0
NA
0(160)
NA
NA
T2/T303
158/189; 1200
136
60/15
5(16)
31.25
NA
5(5)
66.67
D: 5-33; I: 3-10; M: 3.
T454/T6
200/150; 1200
144
59/25
22(25)
88
NA
13(13)
29.92
D: 6-62; I: 3-15; M: 3-8.
218
135
WT
D: delete fragment (bp); I: insert fragment (bp); M: mutated fragment (bp). NA: not applicable.
1