B
(1)
COMMENTS
Student:
Instructor:
Date:
Beginning Technique little understanding of technique, unable to
M
(3)
P
(5)
B
BIO 271 TISSUE CULTURE SKILL
M
complete w/o assistance, minimal ability to follow protocol
Minimal Technique room for improvement, understands technique,
minimal assistance needed, able to follow protocol, able to see mistakes
but unclear how to resolve
Proficient Technique understands technique, able to complete
technique w/o assistance, able to follow protocol, sees mistakes and selfcorrects
P
Laminar flow hood
Turned on hood and properly adjusted apron
Kept work surface uncluttered
Kept minimal supplies in hood for culture work
Wiped surface with 70% EtOH before work and after
Personal Hygiene
Used gloves
Used Lab coat
Used eyewear - if necessary
Reagents and media and supplies
Stored reagents/ solutions at correct temperatures
Stored media at correct temperatures
Properly sterilized reagents/media/solutions
Stored flasks/plates/tubes in original packaging Checked solutions for contamination, cloudiness and unusual
color before use
Visually checked pH of media
Warmed solutions prior to use
Basic Aseptic Technique
Used slow deliberate and mindful technique
Kept all sterile items within the appropriate parameters of the
hood
Placed caps or covers face down and to the back of the work area
Cautiously took paper off individually wrapped pipets
Positioned pipet so gradations are visible
Showed care when handling sterile pipet with hands
Did not to touch neck of flask or bottles with tip of pipet when
entering or exiting a bottle
Did not cross over open bottles or flasks with arms, hands or other
items
Kept caps, bottles and flasks in the hood when not in use
Kept pipet tip away from splashing waste
Labeled tubes/flasks/bottles properly
Cleaned up spills immediately and wipes area with 70% EtOH
Demonstrated pipetting skills that were smooth and accurate
Kept liquid out of neck of flasks when handling flasks and pipetting
Made no (or minimal) bubbles when pipetting liquids
Cell lines
Demonstrates knowledge of difference between cell lines used in
class
Demonstrates knowledge of names of cell lines
Knows where to find information for cell lines
Demonstrates ability to obtains single cell suspension by pipetting
up and down gently
Was able to subculture and feed cells
Correctly used an inverted microscope
Used two eyes when using inverted microscope
Was able to focus on adherent and non-adherent cells
Demonstrates knowledge of correct y balanced tubes for
centrifugation
Knew how to set rpm on centrifuge
Covered microscope when work is complete
Quantitation
Carried microscope by neck and base
Able to focus on the grid of a hemocytometer
Stored cover with the light off
Demonstrated correct use a hemocytometer
Correctly counted cells and determined viability
Correctly calculated cells/ml
Correctly calculated cells/ml and total cells
Correctly calculated viability
Growth curve information
Showed understanding of confluent from non-confluent cultures
Was able to follow protocol
Demonstrated understanding of different stages of cell growth
Was able to make accurate cell counts
Cloning
Demonstrated understanding of the purpose of cell cloning
Correctly designed and followed a protocol
Correctly identified well/dish with a cloned cell
Made correct calculations related to experiment
Cryopreservation
Choose correct chemicals used for freezing media
Correctly prepared freezing media
Correctly labeled cryovials
Determined correct temperature and length of initial freezing of
cells
Contamination
Can identify bacterial contamination
Correctly resolved contaminated cultures/media/solutions
Laboratory management
Demonstrated understanding of laboratory personnel structure
Used good project management tactics
Efficiently manipulated online catalogs for ordering supplies
Other Types of Cell Culture
Was able to name different culture systems