fyr12162-sup-0003-TableS2
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Table S1. Primers used in this study Primer name Primer sequence 5’ to 3’ Application pDS-FW GGGAGATCTATGACAAGAGGGTCGAACTCGCCTAAGTCGTAATTGAGT pDS plasmid construction CAGTTACGCTAGGGATAACAGGGTAATATAGCTGTTTAGCTTGCCTCGTCC pDS4-FW GGGGGATCCATGACAAGAGGGTCGAACTCGCCTAAGTCGTAATTGAGT pDS plasmid construction CAGTTACGCTAGGGATAACAGGGTAATATAGAGAGCTCGCTGTGAAGATC C pDS-RV GGGTCTAGACCGCCAAGCGAATTGAAGGACCGTGCGTAGAATGAAGAA pDS plasmid construction CCTATATTACCCTGTTATCCCTAGCGTAACTTTAAGGGTTCTCGAGAGCTC pDS4-RV GGGGAATTCCCGCCAAGCGAATTGAAGGACCGTGCGTAGAATGAAGAA pDS plasmid construction CCTATATTACCCTGTTATCCCTAGCGTAACTAGAGATCCGCAGGCTAACCG pUG-FW GGGTCTAGAATGACAAGAGGGTCGAACTCGCCTAAGTCGTAATTGAGT pDS plasmid construction CCAGATCCACTAGTGGCCTATG pUG4-FW GGGGAATTCATGACAAGAGGGTCGAACTCGCCTAAGTCGTAATTGAGT pDS plasmid construction CCAGATCCACTAGTGGCCTATG pUG-RV GGGAGATCTCCGCCAAGCGAATTGAAGGACCGTGCGTAGAATGAAGAA pDS plasmid construction CATTAAGGGTTGTCGACCTGC pUG4-RV GGGGGATCCCCGCCAAGCGAATTGAAGGACCGTGCGTAGAATGAAGAA pDS plasmid construction CATTAAGGGTTGTCGACCTGC ura3_Sm_fw ATATATACGCATATGTAGTGTTGAAGAAACATGAAATTGCCCAGTATT URA3 Sm deletion cassette CTTAACCCAACTGCACAGAACAAAAACCTGCAGGAAACGAAGATAAA TCATGTTGAGTCAGTTACGCTAGGG ura3_Sm_rv AATTGAAGCTCTAATTTGTGAGTTTAGTATACATGCATTTACTTATAAT URA3 Sm deletion cassette ACAGTTTTTTAACCAATCTAAGTCTGTGCTCCTTCCTTCGTTCTTCCTTCTCGT GCGTAGAATGAAGAACC ade2_Sm_fw TGTATAAATTGGTGCGTAAAATCGTTGGATCTCTCTTCTAAGTACATC ADE2 Sm deletion cassette CTACTATAACAATCAAGAAAAACAAGAAAATCGGACAAAACAATCAAG TATGTTGAGTCAGTTACGCTAGGG ade2_Sm_rv AATAGGTATATCATTTTATAATTATTTGCTGTACAAGTATATCAATAAA ADE2 Sm deletion cassette CTTATATATTATGAAATGCTCCATAATATTGTCCATTTAGTTCTTAATAAACGTG CGTAGAATGAAGAACC can1_Sm_fw GGATCCAGTTTTCAATCTGTCGTCAATCGAAAGTTTATTTCAGAGTTC TTCAGACTTCTTAACTCCTGTAAAAACAAAAAAAAAAAAAGGCATAGC AATGTTGAGTCAGTTACGCTAGGG CAN1 Sm deletion cassette can1_Sm_rv GAGGGTGAGAATGCGAAATGGCGTGGGAATGTGATTAAAGGTAATAA CAN1 Sm deletion cassette AACGTCATATCTAAACTATAAGTATAATAGTAACTTATATATTTCTGTTCCAGC GTGCGTAGAATGAAGAACC ura3_AB_fw ATATATACGCATATGTAGTGTTGAAGAAACATGAAATTGCCCAGTATT URA3 AB deletion cassette CTTAACCCAACTGCACAGAACAAAAACCTGCAGGAAACGAAGATAAA TCATGCAGCTGAAGCTTCGTACGC ura3_AB_rv TTACGACCGAGATTCCCGGGTAATAACTGATATAATTAAATTGAAGCT URA3 AB deletion cassette CTAATTTGTGAGTTTAGTATACATGCATTTACTTATAATACAGTTTTTT AGCATAGGCCACTAGTGGATCTG ade2_AB_fw TGTATAAATTGGTGCGTAAAATCGTTGGATCTCTCTTCTAAGTACATC ADE2 AB deletion cassette CTACTATAACAATCAAGAAAAACAAGAAAATCGGACAAAACAATCAAG TATGCAGCTGAAGCTTCGTACGC ade2_AB_rv GAAGTCCACATTTGATGTAATCATAACAAAGCCTAAAAAATAGGTATA ADE2 AB deletion cassette TCATTTTATAATTATTTGCTGTACAAGTATATCAATAAACTTATATATT AGCATAGGCCACTAGTGGATCTG can1_AB_fw TCCAATAGGTGGTTAGCAATCGTCTTACTTTCTAACTTTTCTTACCTTT CAN1 AB deletion cassette TACATTTCAGCAATATATATATATATATTTCAAGGATATACCATTCTAA TGCAGCTGAAGCTTCGTACGC can1_AB_rv CTAAAAGAGAGTCGGATGCAAAGTTACATGGTCTTAAGTTGGCGTAC CAN1 AB deletion cassette AATTGAAGTTCTTTACGGATTTTTAGTAAACCTTGTTCAGGTCTAACA CTAGCATAGGCCACTAGTGGATCTG UdcFW GCTACTGCGCCAATTGATGAC Confirmation of deletion and marker excision in URA3 locus KanA CGCACGTCAAGACTGTCAAG Confirmation of URA3, ADE2 and CAN1 deletions KanB TCGTATGTGAATGCTGGTCG Confirmation of URA3 deletion UdcRV CGAGATTCCCGGGTAATAACTG Confirmation of deletion and marker excision in URA3 locus AdcFW AAAGGACACCTGTAAGCGTTG Confirmation of deletion and marker excision in ADE2 locus NatRV CTGTAGGTCAGGTTGCTTTC Confirmation of ADE2 deletion AdcRV AACGCCGTATCGTGATTAAC Confirmation of deletion and marker excision in ADE2 locus CdcFW TTCTAGGTTCGGGTGACGTGAAG Confirmation of deletion and marker excision in CAN1 locus hygroFW GGACGCTCGAAGGCTTTAAC Confirmation of CAN1 deletion CdcRV GGTTGCGAACAGAGTAAACC Confirmation of deletion and marker excision in CAN1 locus FK157-Fb1 GCGGATAAAGTTGCAGGAC Confirmation of yeast transformation with pUDC073 I-SceI inside rv GAACCAGTATGCCAGAGACATC Confirmation of yeast transformation with pUDC073 UdcFW GCTACTGCGCCAATTGATGAC Sequencing of URA3 locus UdcRV CGAGATTCCCGGGTAATAACTG Sequencing of URA3 locus AdcRV AACGCCGTATCGTGATTAAC Sequencing of ADE2 locus AdcFW AAAGGACACCTGTAAGCGTTG Sequencing of ADE2 locus CdcRV GGTTGCGAACAGAGTAAACC Sequencing of CAN1 locus CdcFW TTCTAGGTTCGGGTGACGTGAAG Sequencing of CAN1 locus 3988 CAATTCAACGCGTCTGTGAG Sequencing of pDS1 and pDS6 3890 GTTCTTCATTCTACGCACGGTCC Sequencing of pDS2 3811 CTCGGTGAGTTTTCTCCTTCAT Sequencing of pDS1 3751 GGTCAGCAGTACAGAACCGTCG Sequencing of pDS3 3725 CCTCAGTGGCAAATCCTAAC Sequencing of pDS2 and pDS7 3219 GTATCACGAGGCCCTTTC Sequencing of pDS1, 3, 4, 5, 6 and 7 2528 TCTTTCCTGCGTTATCCC Sequencing of pDS1-7 2498 ATACGACCCAGAAGCTTACC Sequencing of pDS2 2363 TTACCACCATCCAATGCAGAC Sequencing of pDS3 1937 CGAATTGCTTGCAGGCATCTC Sequencing of pDS5 1936 AATCTCGTGATGGCAGGTTGG Sequencing of pDS5 1166 GCTGGAGGTCACCAACGTCAAC Sequencing of pDS6 1077 AGCTTCCCTACCTGACACTAAC Sequencing of pDS4 1076 CACGTGACTGCGCTGAATTG Sequencing of pDS4 526 CTCGCCGATAGTGGAAACCG Sequencing of pDS7 Italic: XbaI or BglII or BamHI or EcoRI recognition sites. Bold: A or B fragments for AB synthetic repeats. Underlined: I-SceI recognition sequence. Bold and underlined: sequence for targeted homologous recombination. Italic and underlined: seamless repeats for marker excision.
