MLAB 2321 Molecular Diagnostics for Clinical Laboratory Science
Unit 12 DNA Sequencing Methods
Name ______________________ (Save file with your name at the end) Points: ________/50
NOTE: Please use bullets for the list of steps in the procedure. The helpful notes are for your use and are ungraded.
Direct
Sequencing
Chemical
Sequencing
(MaxamGilbert)
Dideoxy Chain
termination
Sequencing
(Sanger)
Dye Primer – a
type of
Dideoxy Chain
termination
Sequencing
Dye terminator
type of
Dideoxy Chain
termination
Sequencing
Pyrosequencing
Bisulfite DNA
sequencing or
methylationspecific
sequencing
Type
Briefly describe and list the steps in the
procedure.
Helpful Notes
Manual
method
5 points
Manual
method
6 points
Automated
Fluorescent
Sequencing
Method
6 points
Automated
Fluorescent
Sequencing
Method
5 points
8 points
Modification
of chain
termination
8 points
Sequencing
Briefly describe and list the steps in the
procedure.
RNA sequencing 8 points
Next Generation Sequencing
2 points –Description only
Human Genome Project
Project
Hierarchal Shotgun
sequencing 2 points
Whole-genome Shotgun
Sequencing 2 points
Institution
NIH (National Institute of
Health)
TIGR (The Institute for
Genomic Research) and
Celear
BRIEF Description
Helpful Notes