ANWARUL-ULOOM DEGREE COLLEGE (AUTONOMOUS)
Biotechnology Syllabus
B.Sc Ist Year (2015-2016)
Semester – I
Total Teaching hours: 60
GENETICS
UNIT-I
CELL STRUCTURE (15 Hours)
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Introduction to BioTechnology, History, Nature, scope and future prospective.
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Cells as basic units of living organisms – Viral, Bacterial, Fungal, Plant and
Animal cells.
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Ultra structure of prokaryotic cells (Cell membrane, Plasmids)
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Ultra structure of Eukaryotic cells (Cell membrane, Mitochondria, Chloroplast,
Endoplasmic reticulum, Golgi complex, Vacuoles).
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Cell division and cell cycle and check points.
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Significance of Mitosis and Meiosis.
UNIT-II
CHROMOSOMAL STRUCTURE AND ORGANIZATION.(15 Hours)
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Chromosome Organization in prokaryotes
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Chromosome Organization in Eukaryotes.
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Nucleus in prokaryotes and Eukaryotes.
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Euchromatin and Heterochromatin
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Morphology of Chromosomes.
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Structure of specialized chromosomes – polytene and lamp brush.
UNIT-III
MENDEL’S LAWS ( 15 Hours)
 Mendel’s Experiments – Factors contributing to the success of Mendel’s
Experiments.
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Law of Segregation assortment – Monohybrid ratio.
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Law of Independent assortment – Dihybrids, Trihybrids.
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Derivation from Mendel’s Law – Partial or Incomplete, Dominance, Codominance.
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Penetrance and Expressivity, Pleotropism.
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Epistatic gene interaction – Modified dihybrid ratio’s(12:3:1; 15:1; 9:3:4; 9:6:1;
13:3)
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Genes and Environment – Phenocopies.
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Pedigree Analysis. Pedigree construction, Autosomal dominant, Autosomal
recessive.
UNIT-IV
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LINKAGE and CROSSING OVER(15 Hours)
Linkage and Recombinant - Discovery of linkage cytological proof of crossing
over recombination.
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Frequency and map distance
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Interference and coincidence.
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- Crossing over in Drosophila
Mechanism of Sex determination – Genetic balance theory – Drosophila
Environmental control of sex determination-Bonelia, Lizards.
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Homogametic and heterogametic sex (human, mammalian, birds).
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X-Linked inheritance (Ex: Hemophilia).
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Y linked inheritance- Holandric Genes
Anwarul uloom Degree College(Autonomous)
Bio Technology Syllabus
B.Sc I year –(2015-2016)
Semester II
Total teaching Hours: 60
Nucleic Acids and Bioinformatics
Unit-I:- Nucleic Acids.(15 Hrs)
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Structure of DNA-Watson and Crick model
Forms of DNA-A, B&Z forms of DNA, Super coiled and related DNA-Role of
topoisomerases.
DNA Replication-Models of DNA replication (semi conservative, non
Conservative models)
Mechanism of DNA-Replication-Linear and circular-Rolling circle and theta
Mechanism of replication.
DNA as Genetic material Griffth’s Avery McCleod,Hershey and Chase Expt.
RNA as Genetic material-Tobacco mosaic Virus.
Unit-II:- DNA Damage & repair. (15 Hrs)
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Consequences of DNA damage.
Types of DNA Damages –single base and double base alterations, chain breaks and cross
linkages.
Mutations.
Types of mutation-. Point and frame shift mutations
Repair of DNA
Types of repair-Base Excision repair, Nucleotide excision repair, mismatch repair, and
double strand beak repair.
Unit-III:-Concept of Biostatistics (15 Hrs)
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Concept of probability, basic laws and its application to Mendelian
Segregation.
Concept of probability distribution, Binomial and poisson distribution,
Normal distribution and their application to biology.
Concept of sampling and sampling distribution. Concept of test of hypothesis
t-test, Application of t-test statistics to biological problems/data:-chi square,
Statistics, application in biology
Concept of analysis of variance.
Unit-IV:-Introduction to Bioinformatics (15 Hrs)
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Introduction to Bioinformatics
Biological database.
Nucleotide sequence and protein database, their utilization in biotechnology
Storage of database in data banks data retrievals from data bases & their
Utilization.
Bioinformatics & Internet.
Applications of Bioinformatics.
Time: 2Hrs
Anwarul uloom Degree college(Autonomous)
B.Sc- I year –(2015-2016)
Bio Technology Semester I & II
Model Paper
Max/Min Marks: 30/12
Section A
Short Answers/Concept based Questions.
Answer all questions
1)
2)
3)
4)
5)
5x2=10
Unit-I
Unit-II
Unit-III
Unit-IV
Choose one from any one unit
Section- B
(Essay Type Questions)
Answer all questions
6) Unit-I
OR
Unit-I
7) Unit-II
OR
Unit-II
8) Unit-III
OR
Unit-III
9) Unit-IV
OR
Unit-IV
4x5=20
Anwarul uloom Degree College(Autonomous)
B.Sc I year –(2015-2016)
Semester I & II
Practical syllabus
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Demonstration of mitosis using root tips of Alium cepa
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Monohybrid and dihybrid ratio in Drosophila/maize
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Estimation of DNA by diphenyl amine method.
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Estimation of RNA by orcinol method.
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Salivary gland chromosome in Drosophila larvae
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Isolation of Auxotropic mutants in E.Coli.
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Histograms.
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Frequency polygon
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Pie diagram.
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Test of Significance,
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X2 test: Test of goodness of fit
Test of independence
Test of Linkage.