Supplemental digital content 3: Table of MLST genotype analysis of C. gattii isolated from our patient. Multilocus sequence typing (MLST) profiles of C. gattii isolates Molecular type Name VGI VGI VGI WM276 EJB86 EJB 1+2 R265 VGIIa SXI1α IGS TEF1 GPD1 LAC1 CAP10 PLB1 MPD1 Strain origin 1,354 bp 7 7 7 740 bp 3 3 3 700 bp 4 4 4 547 bp 5 5 14 554 bp 5 5 5 568 bp 4 4 4 600 bp 5 5 5 677 bp 3 3 3 South Australia Rhode Island, USA North Carolina, USA 18 4 7 1 4 1 1 5 Vancouver Island, Can. VGIIb R272 19 10 5 6 4 1 2 5 Vancouver Island, Can. VGIIc EJB18 35 15 7 6 4 1 1 5 Oregon, USA MLST profile of the patient isolate (EJB86). MLST analysis at eight unlinked genomic loci illustrates that the patient isolate is VGI molecular type with a commonly found genotype that is distinct from the outbreak VGII genotypes and a VGI genotype reported in a man from North Carolina. Each allele number represents a unique sequence in the GenBank database. Accession numbers for alleles and primers are listed in previous studies1,6,13. NC=North Carolina; Can=Canada