Title: A Case of Cryptococcus gattii genotype VGI infection in New

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Supplemental digital content 3: Table of MLST genotype analysis of C. gattii isolated from our
patient.
Multilocus sequence typing (MLST) profiles of C. gattii isolates
Molecular
type
Name
VGI
VGI
VGI
WM276
EJB86
EJB
1+2
R265
VGIIa
SXI1α
IGS
TEF1
GPD1
LAC1
CAP10
PLB1
MPD1
Strain origin
1,354
bp
7
7
7
740
bp
3
3
3
700
bp
4
4
4
547
bp
5
5
14
554
bp
5
5
5
568 bp
4
4
4
600
bp
5
5
5
677
bp
3
3
3
South Australia
Rhode Island, USA
North Carolina, USA
18
4
7
1
4
1
1
5
Vancouver Island,
Can.
VGIIb
R272
19
10
5
6
4
1
2
5
Vancouver Island,
Can.
VGIIc
EJB18
35
15
7
6
4
1
1
5
Oregon, USA
MLST profile of the patient isolate (EJB86). MLST analysis at eight unlinked genomic loci illustrates that the patient isolate is VGI
molecular type with a commonly found genotype that is distinct from the outbreak VGII genotypes and a VGI genotype reported in a
man from North Carolina. Each allele number represents a unique sequence in the GenBank database. Accession numbers for
alleles and primers are listed in previous studies1,6,13. NC=North Carolina; Can=Canada
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