Supplemental Tables
Supplemental Table S1: Sequences of the target gene primers
Target genes Primers Base sequence (5'to3')
DDR1 F ACTTTGGCATGAGCCGGAAC
R ACGTCACTCGCAGTCGTGAAC
GAPDH F TGCACCACCAACTGCTTAGC
R GGCATGGACTGTGGTCATGAG
Supplemental Table S2: Summary of genes with > 2 fold differences between the ER and nER patient groups
Gene name
Gene
acronym
TACSTD1
Fold
Molecular_function
8.31
VNN1
4.63
homotypic calcium-independent cell adhesion
molecule
hydrolase activity, acting on carbon-nitrogen
(but not peptide) bonds// GPI anchor binding
Glypican 3
GPC3
3.84
GPI anchor binding
Epiplakin 1
EPPK1
3.75
structural molecule activity
Sulfotransferase family, cytosolic, 1C,
member 1
Leucine rich repeat containing 1
SULT1C1
3.61
sulfotransferase activity
LRRC1
3.48
protein binding
Ectonucleotide
pyrophosphatase/phosphodiesterase 5
ENPP5
3.45
hydrolase activity
Zinc finger protein 528
ZNF528
3.38
nucleic acid binding // zinc ion binding
UDP glucuronosyltransferase 1 family,
polypeptide A8 /// UDP
glucuronosyltransferase 1 family,
polypeptide A9
Chromosome 1 open reading frame 106
UGT1A8 ///
UGT1A9
3.34
UDP-glycosyltransferase activity
C1orf106
3.25
LON peptidase N-terminal domain and ring
finger 2
SRY (sex determining region Y)-box 4
LONRF2
2.92
SOX4
2.91
Solute carrier family 44, member 3
SLC44A3
2.91
Phosphoserine phosphatase
PSPH
2.90
Hexokinase domain containing 1
HKDC1
2.90
Pleckstrin homology-like domain, family
A, member 2
CD24 molecule
PHLDA2
2.74
CD24
2.71
receptor activity// protein binding
Protocadherin 20
PCDH20
2.69
calcium ion binding // protein binding
Papilin, proteoglycan-like sulfated
glycoprotein
PAPLN
2.65
metalloendopeptidase activity //serine-type
endopeptidase inhibitor activity // protein
binding
WW domain binding protein 5
WBP5
2.65
Tumor-associated calcium signal
transducer 1
Vanin 1
ATP-dependent peptidase activity// protein
binding// zinc ion binding
transcription factor activity// DNA binding
phosphoserine phosphatase activity//
hydrolase activity// catalytic activity
nucleotide binding // transferase activity
Kelch-like 13 (Drosophila)
KLHL13
2.61
Calmodulin binding transcription activator
1
Dystrobrevin, alpha
CAMTA1
2.60
DTNA
2.55
X-prolyl aminopeptidase (aminopeptidase
P) 2, membrane-bound
XPNPEP2
2.55
Drebrin 1
DBN1
2.48
Frizzled homolog 7 (Drosophila)
FZD7
2.46
Chromosome 8 open reading frame 72
C8orf72
2.44
Zinc finger protein 204
ZNF204
2.43
Discoidin domain receptor family, member
1
DDR1
2.42
Zinc finger protein 610
ZNF610
2.42
Mitogen-activated protein kinase 13
MAPK13
2.34
ADAM metallopeptidase with
thrombospondin type 1 motif, 5
(aggrecanase-2)
Olfactomedin-like 3
ADAMTS5
2.33
OLFML3
2.32
Collagen, type V, alpha 2
COL5A2
2.30
Hypothetical protein LOC120376
LOC120376
2.28
Transcribed locus
protein binding
calcium ion binding // protein binding // zinc
ion binding
metalloexopeptidase activity // X-Pro
aminopeptidase activity // metal ion binding //
GPI anchor binding // metallopeptidase
activity // hydrolase activity
actin binding // profilin binding // protein
binding
G-protein coupled receptor activity // signal
transducer activity // oxidoreductase activity
nucleic acid binding // zinc ion binding //
metal ion binding
nucleotide binding // transmembrane receptor
protein tyrosine kinase activity // ATP binding
nucleic acid binding // zinc ion binding //
metal ion binding
nucleotide binding // protein-tyrosine kinase
activity // MAP kinase activity // protein
binding // ATP binding // transferase activity
metalloendopeptidase activity // integrin
binding // zinc ion binding // protein
prenyltransferase activity // hydrolase activity
extracellular matrix structural constituent //
structural molecule activity
2.26
Chromosome 5 open reading frame 13
C5orf13
2.26
protein binding // tRNA binding // structural
constituent of ribosome
Unc-5 homolog C (C. elegans)-like
UNC5CL
2.24
protein binding
Quiescin Q6
QSCN6
2.20
electron carrier activity // protein disulfide
oxidoreductase activity // thiol oxidase
activity
CDNA FLJ33420 fis, clone
BRACE2020028
Feline leukemia virus subgroup C cellular
receptor
2.20
FLVCR
2.19
EPS8-like 3
EPS8L3
2.18
Glutathione S-transferase M3 (brain)
GSTM3
2.17
receptor activity // transporter activity
glutathione transferase activity
Stanniocalcin 1
STC1
2.17
protein kinase activity // hormone activity //
ATP binding
cyclin-dependent protein kinase inhibitor
activity // protein binding
Cyclin-dependent kinase inhibitor 1C (p57,
Kip2)
CDKN1C
2.17
Solute carrier family 7 (cationic amino acid
transporter, y+ system), member 1
SLC7A1
2.15
Pleckstrin homology domain containing,
family H (with MyTH4 domain) member 2
PLEKHH2
2.12
Nebulette
NEBL
2.11
actin binding // zinc ion binding // structural
constituent of muscle // metal ion binding
Geminin, DNA replication inhibitor
GMNN
2.08
protein binding
Glutathione S-transferase A4
GSTA4
2.08
glutathione transferase activity
Vang-like 2 (van gogh, Drosophila)
VANGL2
2.07
protein binding
Laminin, beta 1
LAMB1
2.06
extracellular matrix structural constituent //
protein binding // structural molecule activity
Cadherin 6, type 2, K-cadherin (fetal
kidney)
Prickle homolog 2 (Drosophila)
CDH6
2.06
calcium ion binding // protein binding
PRICKLE2
2.05
zinc ion binding // metal ion binding
Hypothetical protein LOC727916
LOC727916
2.05
E2F transcription factor 3
E2F3
2.04
NCK adaptor protein 2
NCK2
2.03
Tight junction protein 3 (zona occludens 3)
TJP3
2.03
protein binding
Jub, ajuba homolog (Xenopus laevis)
JUB
2.02
zinc ion binding // metal ion binding
Choroideremia-like (Rab escort protein 2)
CHML
2.01
Rab-protein geranylgeranyltransferase activity
// Rab escort protein activity // GTPase
activator activity // rhodopsin-like receptor
activity // signal transducer activity // Gprotein coupled receptor activity //
photoreceptor activity
receptor activity // amino acid-polyamine
transporter activity // basic amino acid
transporter activity // amino acid permease
activity
binding
transcription factor activity // protein binding
// DNA binding
protein binding // cytoskeletal adaptor activity
// receptor signaling complex scaffold activity
Small trans-membrane and glycosylated
LOC57228
2.01
protein
Supplemental Table S3: 49 genes down-regulated in the ER group by more than 2-fold compared with that in the
nER group.
Gene name
Gene symbol
Fold Molecular_function
hydroxyprostaglandin
dehydrogenase 15-(NAD)
HPGD
0.11
prostaglandin-D synthase activity // prostaglandin E
receptor activity // 15-hydroxyprostaglandin
dehydrogenase (NAD+) activity // oxidoreductase
activity // protein homodimerization activity // NAD
binding // catalytic activity
membrane metalloendopeptidase (neutral
endopeptidase, enkephalinase)
autocrine motility factor
receptor
MME
0.24
neprilysin activity // metallopeptidase activity // zinc
ion binding // metal ion binding // hydrolase activity
AMFR
0.24
synovial sarcoma, X breakpoint
1
Zinc finger protein 718
SSX1
0.24
ubiquitin-protein ligase activity // receptor activity //
protein binding // zinc ion binding // ligase activity //
metal ion binding
nucleic acid binding // transcription corepressor activity
ZNF718
0.25
ATP-binding cassette, subfamily A (ABC1), member 8
ABCA8
0.26
cytochrome c oxidase subunit
VIIb2
serine dehydratase
COX7B2
0.26
SDS
0.26
Adrenergic, alpha-1A-, receptor
ADRA1A
0.29
phosphoenolpyruvate
carboxykinase 1 (soluble)
PCK1
0.30
complement factor H-related 4
CFHR4
0.32
nucleic acid binding // DNA binding // zinc ion binding
// metal ion binding
nucleotide binding // ATP binding // ATPase activity,
coupled to transmembrane movement of substances //
nucleoside-triphosphatase activity
cytochrome-c oxidase activity // oxidoreductase
activity
catalytic activity // L-serine ammonia-lyase activity //
lyase activity
rhodopsin-like receptor activity // receptor activity //
adrenoceptor activity // signal transducer activity // Gprotein coupled receptor activity // melanocortin
receptor activity
nucleotide binding // phosphoenolpyruvate
carboxykinase (GTP) activity // GTP binding //
carboxy-lyase activity
lipid transporter activity
cystathionase (cystathionine
gamma-lyase)
BCL2-like 10 (apoptosis
facilitator)
ATP-binding cassette, subfamily B (MDR/TAP), member
11
CTH
0.33
cystathionine gamma-lyase activity // lyase activity
BCL2L10
0.34
protein binding
ABCB11
0.35
bone marrow stromal cell
antigen 2
G protein-coupled receptor 125
BST2
0.36
nucleotide binding // transporter activity // ATP
binding // sodium-exporting ATPase activity,
phosphorylative mechanism // bile acid-exporting
ATPase activity // ATPase activity // nucleosidetriphosphatase activity // ATPase activity, coupled to
transmembrane movement of substances
signal transducer activity // GPI anchor binding
GPR125
0.36
receptor activity // G-protein coupled receptor activity
// protein binding // signal transducer activity
aquaporin 9
AQP9
0.37
lymphocyte antigen 96
LY96
0.38
amine transporter activity // purine transporter activity
// pyrimidine transporter activity // polyol transporter
activity // porin activity // carboxylic acid transporter
activity // water transporter activity // transporter
activity // water channel activity
coreceptor activity
glutamic pyruvate transaminase
(alanine aminotransferase) 2
GPT2
0.38
alanine transaminase activity // 1-aminocyclopropane1-carboxylate synthase activity // transaminase activity
// transferase activity, transferring nitrogenous groups
apolipoprotein A-V
APOA5
0.39
lipid transporter activity // triglyceride binding // lipid
binding
transforming growth factor, beta
receptor III (betaglycan,
300kDa)
SEC14-like 2 (S. cerevisiae)
TGFBR3
0.40
receptor activity // glycosaminoglycan binding
SEC14L2
0.41
mitochondrial ribosomal protein
L43
nudix (nucleoside diphosphate
linked moiety X)-type motif 16
pseudogene
arginase, type II
MRPL43
0.41
NUDT16P
0.41
transporter activity // carrier activity // phospholipid
binding // vitamin E binding // transcriptional activator
activity //lipid binding
structural constituent of ribosome // translation
regulator activity
hydrolase activity
ARG2
0.42
steroid-5-alpha-reductase, alpha
polypeptide 2
SRD5A2
0.42
CD1d molecule
CD1D
0.42
aminoadipate-semialdehyde
synthase
AASS
0.42
hect domain and RLD 5
HERC5
0.43
zinc finger and BTB domain
containing 16
ZBTB16
0.43
ST3 beta-galactoside alpha-2,3sialyltransferase 6
ST3GAL6
0.43
estrogen receptor 1
ESR1
0.43
mitogen-activated protein
kinase kinase kinase 5///
hypothetical protein
LOC729144 /// hypothetical
protein LOC732274
MAP3K5///
LOC729144 ///
LOC732274
0.44
EF-hand domain family,
member A2
absent in melanoma 1
EFHA2
0.44
transcription factor activity // steroid hormone receptor
activity // steroid binding // protein binding // zinc ion
binding // lipid binding // nitric-oxide synthase
regulator activity //estrogen receptor activity //
sequence-specific DNA binding // metal ion binding //
DNA binding // receptor activity // ligand-dependent
nuclear receptor activity
nucleotide binding // magnesium ion binding // protein
serine/threonine kinase activity // MAP kinase kinase
kinase activity // ATP binding // caspase activator
activity // transferase activity // identical protein
binding // protein binding // protein kinase activity //
kinase activity // metal ion binding
calcium ion binding
AIM1
0.44
molecular_function // sugar binding
follistatin
FST
0.44
protein binding // activin inhibitor activity
SEC14-like 2 (S. cerevisiae)
SEC14L2
0.45
transporter activity // carrier activity // phospholipid
binding // vitamin E binding // transcriptional activator
activity // lipid binding
arginase activity // hydrolase activity // manganese ion
binding // metal ion binding // hydrolase activity, acting
on carbon-nitrogen (but not peptide) bonds, in linear
amidines
3-oxo-5-alpha-steroid 4-dehydrogenase activity // sterol
5-alpha reductase activity // oxidoreductase activity
receptor activity // beta-2-microglobulin binding //
exogenous lipid antigen binding // protein
heterodimerization activity
peroxidase activity // lysine-ketoglutarate reductase
activity // oxidoreductase activity // saccharopine
dehydrogenase (NADP+, L-lysine-forming) activity //
catalytic activity
ubiquitin-protein ligase activity // ligase activity
DNA binding // zinc ion binding // specific
transcriptional repressor activity // protein
homodimerization activity // metal ion binding //
protein binding // identical protein binding // nucleic
acid binding
sialyltransferase activity // transferase activity //
transferase activity, transferring glycosyl groups
ST3 beta-galactoside alpha-2,3sialyltransferase 6
ST3GAL6
0.45
sialyltransferase activity // transferase activity //
transferase activity, transferring glycosyl groups
apolipoprotein C-IV
APOC4
0.46
adrenergic, beta-2-, receptor,
surface
ADRB2
0.46
alcohol dehydrogenase 6 (class
V)
ADH6
0.47
v-myc myelocytomatosis viral
oncogene homolog 1, lung
carcinoma derived (avian)
ATP-binding cassette, subfamily C (CFTR/MRP),
member 9
MYCL1
0.47
lipid transporter activity // enzyme activator activity //
lipoprotein lipase activity
rhodopsin-like receptor activity // receptor activity //
beta2-adrenergic receptor activity // protein binding //
protein homodimerization activity // norepinephrine
binding //signal transducer activity // G-protein coupled
receptor activity // adrenoceptor activity
alcohol dehydrogenase activity // zinc ion binding
//electron carrier activity // oxidoreductase activity //
metal ion binding
transcription factor activity // DNA binding
ABCC9
0.47
mitogen-activated protein
kinase kinase kinase 5
MAP3K5
0.48
peroxisome proliferatoractivated receptor gamma,
coactivator 1 alpha
PPARGC1A
0.48
sorting nexin 10
SNX10
0.49
CD72 molecule
CD72
0.50
transmembrane receptor activity // receptor binding //
sugar binding // protein binding // receptor activity
Ceruloplasmin (ferroxidase)
CP
0.50
splicing factor, arginine/serinerich 2B
SFRS2B
0.50
ferroxidase activity // copper ion transporter activity //
copper ion binding // oxidoreductase activity // metal
ion binding
nucleotide binding
nucleotide binding // receptor activity // transporter
activity // ATP binding // sulfonylurea receptor activity
// ATPase activity, coupled to transmembrane
movement of substances
nucleotide binding // magnesium ion binding // protein
serine/threonine kinase activity // MAP kinase kinase
kinase activity // ATP binding // caspase activator
activity // transferase activity // identical protein
binding // protein binding // protein kinase activity //
kinase activity //metal ion binding
nucleotide binding // DNA binding // RNA binding //
transcription factor binding // RNA polymerase II
transcription mediator activity // ligand-dependent
nuclear receptor transcription coactivator activity //
androgen receptor binding // protein binding
protein binding // phosphoinositide binding