Wildenberg, Murray Supplementary File 1
Strain name
yGW 461
yGW E6
yGW E15
yGW 5A7
yGW 501
yGW 556
Genetic change
MATa BUD4 HIS3∆::PACT1-ymCherry-tADH1-HIS3MX6
PFLO1::PFLO1-ymCitrine-tADH1 pol3-L523D leu2-3,112 ura3∆0
can1-100
Ancestor genotype: evolved 360 generations
Ancestor genotype: evolved 360 generations
Ancestor genotype: evolved 360 generations
PACT1-ymCerulan-tADH1
MATalpha lys2∆::Hygror
yGW 451
Ancestor with wild type POL3
yGW 992
MATa BUD4 HIS3∆::PACT1-ymCherry-tADH1- HIS3∆::MX6
PFLO1::PFLO1-ymCitrine-tADH1 leu2-3,112 ura3∆0 can1-100
hbn1 ids2 pet127 scw11 sir4 vps5 whi2-100 yjl070c
hml∆::Ca.URA3
Ancestor genotype: evolved 360 generations
hml∆::Ca.URA3
Ancestor genotype: evolved 360 generations
hml∆::Ca.URA3
Ancestor genotype: evolved 360 generations
hml∆::Ca.URA3
Ancestor genotype: evolved 360 generations
hml∆::Ca.URA3
Ancestor genotype: evolved 360 generations
hml∆::Ca.URA3
Ancestor genotype: evolved 360 generations
hml∆::Ca.URA3
Ancestor genotype: evolved 360 generations
hml∆:: Ca.URA3
Ancestor genotype: evolved 360 generations
hml∆::Ca.URA3
hbn1∆:: KANr
ids2∆:: KANr
pet127∆:: KANr
scw11∆:: KANr
sir4∆:: KANr
vps5∆:: KANr
whi2-100∆:: KANr
yjl070c∆:: KANr
Ancestor genotype: evolved 360 generations
Ancestor genotype: evolved 360 generations
Ancestor genotype: evolved 360 generations
Ancestor genotype: evolved 360 generations
Ancestor genotype: evolved 360 generations
Ancestor genotype: evolved 360 generations
Ancestor genotype: evolved 360 generations
Ancestor genotype: evolved 360 generations
Ancestor genotype: evolved 360 generations
Ancestor genotype: evolved 360 generations
Ancestor genotype: evolved 360 generations
Ancestor genotype: evolved 360 generations
Ancestor genotype: evolved 360 generations
Ancestor genotype: evolved 360 generations
Ancestor genotype: evolved 360 generations
Ancestor genotype: evolved 360 generations
yGW 861
yGW 1A1
yGW 903
yGW 1G1
yGW 905
yGW 5A1
yGW 900
yGW 5C5
yGW 898
yGW 1B2
yGW 896
yGW 1C3
yGW 894
yGW 1D10
yGW 907
yGW 1E7
yGW 893
yGW 936
yGW 932
yGW 924
yGW 926
yGW 928
yGW 934
yGW 923
yGW930
yGW E6/1A9
yGW E6/5A5
yGW E6/1B8
yGW E6/1B12
yGW E6/1C3
yGW E6/1D10
yGW E6/1H6
yGW E6/5A11
yGW E6/5B4
yGW E6/5B8
yGW E6/5B12
yGW E15/1A7
yGW E15/1E1
yGW E15/1E3
yGW E15/1F6
yGW E15/1G9
Additional Information
Ancestor
Evolved population: 6% selection
Evolved population: 15% selection
E6-1 evolved clone from 6% evolved population
E6-1 + CFP
Mating type switched Ancestor used for crossing
to E6-1 and bulk segregant analysis
Lab strain used to introduce clock alleles: direct
descendant of ancestor prior to introduction of
pol3-L523D
Complete recreated clock strain with all 8
mutations
E6-1 with hml∆
E6-2 clone
E6-2 hml∆
E6-3 clone
E6-3 hml∆
E6-4 clone
E6-4 hml∆
E6-5 clone
E6-5 hml∆
E15-1 clone
E15-1 hml∆
E15-2 clone
E15-2 hml∆
E15-3 clone
E15-3 hml∆
E15-4 clone
E15-4 hml∆
E6-1 hbn1∆
E6-1 ids2∆
E6-1 pet127∆
E6-1 scw11∆
E6-1 sir4∆
E6-1 vps5∆
E6-1 whi2∆
E6-1 yjl070c∆
E6-6
E6-7
E6-8
E6-9
E6-10
E6-11
E6-12
E6-13
E6-14
E6-15
E6-16
E15-5
E15-6
E15-7
E15-8
E15-9
yGW E15/2A1
yGW E15/2B6
yGW E15/2C7
yGW E15/2D8
yGW E15/2E3
yGW E15/2F6
Ancestor genotype: evolved 360 generations
Ancestor genotype: evolved 360 generations
Ancestor genotype: evolved 360 generations
Ancestor genotype: evolved 360 generations
Ancestor genotype: evolved 360 generations
Ancestor genotype: evolved 360 generations
E15-10
E15-11
E15-12
E15-13
E15-14
E15-15