University of Puerto Rico
Río Piedras Campus
College of Natural Sciences
Nutrition and Dietetics Program
Program: Bachelor of Science in
Nutrition and Dietetics
Human Biochemistry Laboratory, NUTR
4159
First Semester, 2011-2012 Academic Year
Sec. 0U1, Thursday, 3:00 p.m. – 5:50 p.m.
Rooms NEP 576 / 577
Michelle Schelske Santos, Ph.D.; mschelske@uprrp.edu; ext. 5798, 2792
Date
Assignment
18Aug11
LAB: Syllabus, Preparation of Lab Notebooks
25Aug11
Academic Recess – Storm/Hurricane Irene
01Sep11
LAB Excel workshop – Data Tables and Graphs;
General Laboratory Safety; Equipment assignment
Intro to instrumentation; (bring lab coats and lab
notebooks)
Quiz in Edu2.0; open 24h before lab
LAB Safety (Biohazard)
Post-conference written quiz; practical lab quiz
Scientific Instrumentation (cont.)
Micropipette and Pipette Techniques
Practical Quiz in lab
LAB Computer Workshop – Info Databases and
your Search Strategy (Biblioteca Ciencias
Naturales; Prof. Pura Centeno)
Assign.: Search Strategies, DNA & Protein
Methods
LAB Expt: Active Acidity and pH;
Quiz in Edu2.0 open 24h before lab
Due: Assignment Search Strategies DNA &
Protein Method
LAB – DNA Extraction
LAB – Tissue Homogenation of Protein Samples
Quiz in Edu 2.0; open 24h before lab
LAB – Total Protein Determination
Quiz in Edu 2.0; open 24h before lab
LAB: Protein Electrophoresis of blood fractions I
Quiz in Edu 2.0; open 24h before lab
08Sep11
15Sep11
22Sep11
29Sep11
06Oct11
13Oct11
20Oct11
Topic
Introduction to Prep of Lab Notebook
Scientific Instrumentation
Introduction to Excel for Lab Reports--Water
content of foods; Lab Safety; Scientific
Instrumentation
Blood-borne Pathogens and Biohazard
Disposal (OPASO; Prof. Jorge Ramos)
Scientific Instrumentation
Micropipette and Pipette Techniques
Biochemical methods for DNA and protein
analyses in the field of nutrition
Active Acidity and pH
DNA Extraction from strawberries
Homogenation of animal & vegetable protein
Protein content of animal versus vegetable
protein on microscale using microplate reader
Preparation of blood fractions
Preparation for and dry run of electrophoresis
Dra. M. Schelske Santos; NUTR 4159
27Oct11
03Nov11
Mon, 07Nov11
5:00-6:00pm
10Nov11
17Nov11
24-26Nov10
01Dec11
TBA, Dec.
12-19, 2011
LAB: Protein Electrophoresis of blood fractions II
Quiz in Edu 2.0; open 24h before lab
LAB: Expt. 30, Vitamin C
Quiz in Edu 2.0; open 24h before lab
LAB OP1 – (50 pts.; 1h)
Protein electrophoresis, gel staining & drying
Colorimetric Determination of Vitamin C: Raw
vs. Processed Foods
Lab safety – Protein Electrophoresis (does not
include Vit. C)
LAB – Expt. Membrane Structure and Function; Blood Typing
Quiz in Blackboard; open 24h before lab;
Digital Microscopy of Blood Cells
LAB Bioinformatics: MMDB, Genes and Disease
3-D structure of proteins, genetic diseases
Academic Recess – HAPPY THANKSGIVING
LAB – Lipids and Fiber
Quiz in Edu 2.0; open 24h before lab
Due Bioinformatics
Lab OP 2 – (50 pts.; 1h)
Due: Lab Notebooks
Dietary Lipids, Fiber & Fattaché
(Vitamin C – Lipids and Fiber)
This schedule is subject to change based on unforeseen events, natural disasters, scheduling of student
assemblies, and changes in laboratory equipment and material arrival dates. In the event of any change, a new
schedule will be developed and available in Edu 2.0.
Edited 23Sep11, MSS
sem. 1, 2011-2012
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