Electronic Supplementary Material
Direct electrochemistry of hemoglobin in a novel and renewable mesoporous
carbon ceramic electrode: A new kind of hydrogen peroxide biosensor
Biuck Habibi*, Mojtaba Jahanbakhshi
Electroanalytical Chemistry Laboratory, Department of Chemistry, Faculty of Sciences,
Azarbaijan Shahid Madani University, Tabriz 53714-161, Iran
*
Corresponding author (Biuck Habibi). Tel: +98 41 34327541; Fax: +98 41 34327541; E-mail:
B.Habibi@azaruniv.edu
Preparation of black CE
The fabricated mesoporous silica was used as the MC template in the synthesizing procedure.
The mentioned template was impregnated by the aqueous solution of glucose containing catalytic
amount of H2SO4 as formerly designated for sucrose [1]. In brief, 1 g of mesoporous template
was added to the 5 mL of aqueous solution containing 1 g of glucose and 0.12 g of H2SO4. The
obtained mixture was situated in an oven at 100 °C for about 6 h and then for another 6 h it was
kept at 160 °C. After refrigeration, the heating accomplishment was repeated, and then another
solution of 0.6 g of glucose and 0.1 g of H2SO4 was added in 5 mL of H2O. The resulting
substance was carbonized at 900 °C under N2 atmosphere by heating degree of 2 °C/min for
about 6 h. At that time the residual silica was eliminated via dissolving the obtained composite in
the 1 M sodium hydroxide ethanolic aqueous solution. The resultant black MC compound was
gathered and stored for characterization and application.
Fig. S1 Plot of peak potential Epa and Epc vs. log (ν).
pH effect on the peak potential of Hb at the Hb/MCCE
The pH effect of the buffer solution on the peak potential of Hb/MCCE was examined by
cyclic voltammetry. As it is shown in S2, both oxidation and reduction peak potentials of the Fe
(III)/Fe (II) redox pair of Hb are negatively shifted by pH values increasing with a slope of -60
mV/pH in the range of pH 3.0-11.0 (Fig. S2B). This slope is practically near to the expected
value of -59 mV/pH for reversible one electron transfer matched with single proton process. So
the redox reaction of Hb entrapped in MCCE can be illustrated as [2]:
HbFe (III) + H+ + e- ↔ HbHFe (II)
Fig. S2. Cyclic voltammograms of Hb/MCCE in various pH solutions 3.0, 4.0, 5.5, 7.0, 8.0, 9.5,
11.0 from right to left, scan rate: 100 mV s-1 (A). Plot of formal potential vs. pH values (B).
Fig. S3. Determination of Michaelis-Menten constant (KM) from the Lineweaver-Burk equation
(1/Iss=KM/ImaxC+1/Imax).
Table 1: Analytical performance of different H2O2 biosensors based on Hb
Hb biosensor
Dynamic range (µM)
Hb-chitosan@Fe3O4/Au
2.3-9600
Hb/Co(OH)2NP/GC
0.4-200
Hb/Au-MFIOH/GCE
1.0- 18000
{Hb/CMK-3}6
1.2-57
Hb-CdS:Mn-CS-GCE
2-18000
Hb-Fe3O4@Pt-CS-GCE
9.2-2000
Hb–Au colloid–cysteamine/Au 0.36-860
Hb/AuNPs–C@SiO2
5-80
Hb–PSMAC/MWCNTs/CPE
1-56
Hb/SA-MWCNTs/GCE
40-200
Hb/AuNPs/PDDA-G
6-1010
Hb/Chit-IL-Fc/Gr/GCE
50-1200
Hb-GNACs/GCE
1.0-140
F-NiO/IL/Hb-CPE
2.0-1050
Hb/mesoTiO2/GCE
2.0-27.5
NP-Au/Hb/GCE
0.05-200
Hb/IL/CILE
100-5000
Hb/NiO/GCE
1.0-2000
Hb/MCCE
1.0-220
Limit of detection (µM)
1.1
0.2
0.8
0.6
0.75
4.5
0.12
0.08
0.38
16.4
0.39
3.8
0.93
0.68
1.0
0.02
40
0.63
0.4
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