Table S4. Plasmids Used in this Study
Plasmid
Insert
Use
pSN147
PmeI-SEF1 promoter fragment-SAT1TDH3 promoter-SEF1 ORF fragmentPmeI
SEF1
overexpression
pSN141
PmeI-SFU1 promoter fragmentCdARG4-TDH3 promoter-SFU1 ORF
fragment-PmeI
PmeI-SSN3 promoter fragment SAT1TDH3 promoter-SSN3 ORF fragmentPmeI
PmeI-LEU2 upstream sequenceCdARG4-TDH3p-SSN3D325A-LEU2
downstream sequence-PmeI
PmeI-SSN3 C-terminal ORF sequence13xMyc-FRT-FLP-SAT1-FRT-SSN3
downstream sequence-PmeI
PmeI-SEF1 C-terminal ORF sequenceTAP-FRT-FLP-SAT1-FRT- SEF1
downstream sequence-PmeI
PmeI-SFU1 C-terminal ORF sequenceTAP-FRT-FLP-SAT1-FRT-SFU1
downstream sequence-PmeI
PmeI-SSN3 C-terminal ORF sequenceTAP-FRT-FLP-SAT1-FRT-SSN3
downstream sequence-PmeI
Pme1-SSN3 C-terminal ORF sequence13xMyc-FRT-FLP-SAT1-FRT-SSN3
downstream sequence-PmeI
PmeI-LEU3 upstream sequence-FRTFLP-SAT1-FRT-TDH3 promoter-TAPLEU2 downstream sequence-PmeI
PmeI-SFU1 upstream flank-FRT-FLPSAT1-FRT-SFU1 downstream flankPmeI
SFU1
overexpression
pSN229
pSN239
pSN218
pSN150
pSN228
pSN219
pSN273
pSN292
pSN184
SSN3
overexpression
SSN3 kinase
dead
Myc-tagged Ssn3
TAP-tagged Sef1
TAP-tagged Sfu1
TAP-tagged Ssn3
Myc-tagged
SSN3 kinase
dead
TAP tag
overexpression
SFU1 Knockout
Selectable
Markers
AmpR,
ScURA3,
NatR
AmpR,
ScURA3,
CdARG4
AmpR,
ScURA3,
NatR
AmpR,
ScURA3,
CdARG4
AmpR,
ScURA3,
NatR
AmpR,
ScURA3,
NatR
AmpR,
ScURA3,
NatR
AmpR,
ScURA3,
NatR
AmpR,
ScURA3,
NatR
AmpR,
ScURA3,
NatR
AmpR,
ScURA3,
NatR
Vector
pRS316
pRS316
pRS316
pRS316
pRS316
pRS316
pRS316
pRS316
pRS316
pRS316
pRS316
PmeI denotes the recognition sequence for this restriction enzyme; CdARG4 denotes the ARG4 gene
from Candida dubliniensis; ScURA3 denotes the URA3 gene from Saccharomyces cerevisiae, AmpR
denotes resistance to ampicillin, NatR denotes resistance to nourseothricin.