Supplementary material
Online Resource 1 Bud formation and plant regeneration from S. laniceps plantlets. a Whole plant
collected from an alpine region in Tibet. b Dry mature seed with flower and coronule. C and d
Mature seed. e Seedlings formed from seeds. f Callus induced from explants of seedlings
Online Resource 2 SDS-PAGE separation of Saussurea involucrata callus proteins extracted by three
sample preparation methods.1.Marker; 2. Extracted by TCA/Aceton precipitation; 3. Extracted by
urea/thiourea extraction; 4. Extracted by phenol extraction-methanol/ammonium acetate
precipitation
Online Resource 3 2-DE separation of Saussurea involucrata callus proteins by three sample
preparation methods. a: protein extracted by TCA/aceton precipitation, b: protein extracted by
urea/thiourea extraction, c: protein extracted by phenol extraction/ammonium acetate
precipitation.
Online Resource 4 2-DE separation of Saussurea involucrata callus proteins by phenol extractionmethanol/ammonium acetate precipitation with different sample volumes a：250
μg，b：500μg，c：600μg
Online Resource 5 Different growth phases of the S. laniceps callus grown in medium supplemented
with 0.5 mg L−1 6-BA and 2.0 mg L−1 1-naphthaleneacetic acid. Error bars represent the standard
deviation of 3 repeated reactions.
Online Resource 6 Changes in soluble protein content in the S. laniceps callus during acclimation at 0
°C. Error bars represent the standard deviation of 3 repeated reactions.
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