Hydrogen peroxide solutions, CAS # 7722-84-1
Standard Operating Procedure
Hydrogen peroxide solutions
This is an SOP template and is not complete until: 1) lab specific information is entered into the box
below 2) lab specific protocol/procedure is added to the protocol/procedure section and
3) SOP has been signed and dated by the PI and relevant lab personnel.
Print a copy and insert into your
Laboratory Safety Manual and Chemical Hygiene Plan.
Refer to instructions for assistance.
School / Department:
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SOP Preparation Date:
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Principal Investigator:
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Lab Manager Name:
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Laboratory Phone:
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Office Phone:
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Emergency Contact:
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Contact Phone:
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SOP Approval Date:
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Laboratory locations covered by this SOP (building / room number):
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Type of SOP:
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Process
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Hazardous Chemical
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Hazardous Class
Purpose
Due to the strong oxidizing properties of Hydrogen peroxide it has several applications. It is often used as a
bleach, cleaning agent, and disinfectant. It is an effective cleaning agent of wastewater. It can also be used as a
rocket propellant. Organisms naturally produce hydrogen peroxide and can decompose small amounts to
useful water and oxygen.
Physical and Chemical Properties / Definition of Chemical Group
CAS:
Class:
Molecular Formula:
Form (physical state):
Color:
Boiling Point:
7722-84-1
Oxidizer, Corrosive, Toxic
H2O2
Liquid
Colorless
108 oC
ASU Department of Environmental Health & Safety
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Hydrogen peroxide solutions, CAS # 7722-84-1
Potential Hazards / Toxicity
Potential Health Effects
Target Organs:
Inhalation:
Skin:
Eyes:
Ingestion:
Eyes, Skin, Respiratory system
May be harmful if inhaled. Material is extremely destructive to the tissue of the
mucous membranes and upper respiratory tract.
May be harmful if absorbed through skin. Causes skin burns.
Causes eye burns.
Toxic if swallowed.
Personal Protective Equipment (PPE)
Respiratory Protection
Use a full-face respirator with multi-purpose combination (US) respirator cartridges as a backup
to engineering controls. If the respirator is the sole means of protection, use a full-face supplied
air respirator.
Respirators should be used only under any of the following circumstances:
 As a last line of defense (i.e., after engineering and administrative controls have been
exhausted).
 When Permissible Exposure Limit (PEL) has exceeded or when there is a possibility that PEL will
be exceeded.
 Regulations require the use of a respirator.
 An employer requires the use of a respirator.
 There is potential for harmful exposure due to an atmospheric contaminant (in the absence of
PEL)
 As PPE in the event of a chemical spill clean-up process
Lab personnel intending to use/wear a respirator mask must be trained and fit-tested by EH&S.
This is a regulatory requirement.
(http://www.asu.edu/uagc/EHS/documents/asu_respriatory_protection_plan.pdf)
Hand Protection
Handle with gloves. Nitrile gloves are recommended.
NOTE: Consult with your preferred glove manufacturer to ensure that the gloves you plan on
using are compatible with Hydrogen peroxide.
Refer to glove selection chart from the links below:
http://www.ansellpro.com/download/Ansell_8thEditionChemicalResistanceGuide.pdf
ASU Department of Environmental Health & Safety
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Hydrogen peroxide solutions, CAS # 7722-84-1
OR
http://www.allsafetyproducts.biz/page/74172
OR
http://www.showabestglove.com/site/default.aspx
OR
http://www.mapaglove.com/
Eye Protection
 Wear chemical splash goggles or a face shield to protect from splash hazards and chemical
vapors. Face shields are recommended.
Skin & Body Protection
 Fire/flame resistant lab coat
 Full-length pants
 Closed-toe rubber or leather shoes
Hygiene Measures
Avoid contact with skin, eyes, and clothing. Wash hands before breaks and immediately after
handling the product.
Engineering Controls
Handle Hydrogen peroxide using a chemical fume hood with good ventilation.
First Aid Procedures
If inhaled… Move to fresh air. If the person is not breathing, give artificial respiration. Avoid
mouth to mouth contact. If breathing is difficult, give oxygen. Call 911 from a campus phone or
(480) 965-3456. Call EH&S at (480) 965-1823.
In case of skin contact… Remove all contaminated clothing. Immediately (within seconds)
flush affected area for FIFTEEN (15) minutes. Call 911 from a campus phone or (480) 965-3456
from a cell phone. Call EH&S at (480) 965-1823.
In case of eye contact… Remove any contact lenses. Use nearest emergency eyewash
immediately for at least 15 minutes. Call 911 from a campus phone or (480) 965-3456 from a cell
phone. Call EH&S at (480) 965-1823. Continue eye rinse during transport to the hospital.
If swallowed… DO NOT INDUCE VOMITING. Give large quantities of milk (preferable) or
water. Rinse mouth with water. Never give anything by mouth to an unconscious person. Call
911 from a campus phone or (480) 965-3456 from a cell phone. Call EH&S at (480) 965-1823.
ASU Department of Environmental Health & Safety
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Hydrogen peroxide solutions, CAS # 7722-84-1
Special Storage & Handling Requirements
Storage
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Ensure the container is tightly closed at all times.
Keep in a dry and well-ventilated area.
Opened containers must be carefully resealed and kept upright to prevent leakage.
Recommended storage temperature is 2-8 oC.
Store protected from light.
Store away from combustible materials.
Avoid alkalies, oxidizable material, alcohols, permanganates, zinc, powdered metals, iron,
copper, nickel, brass, iron, and iron salts.
Handling
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The lab where the material is being handled has an approved / certified emergency eyewash
and safety shower.
Ensure you are wearing the following minimum PPE: tightly fitting safety goggles, fire/flame
resistant lab coat, full length pants, closed-toe rubber or leather shoes, nitrile gloves.
Lab emergency contact information must be readily posted. Easy access to a cellular phone
or land line is readily available.
Handle and open container with care.
Avoid contact with skin, eyes, and clothing.
Avoid inhalation and ingestion.
Ensure adequate ventilation.
Keep away from sources of ignition.
Spill and Accident Procedure
Personal precautions
Avoid breathing vapors, mist or gas. Ensure adequate ventilation. Evacuate personnel to safe
areas. Do not attempt clean-up without minimum PPE.
Environmental precautions
Prevent further leakage or spillage – if safe to do so. Do not allow product to enter drains.
Discharge into the environment must be avoided.
Methods and materials for containment and clean-up
Consider material compatibility prior to clean-up. Verify spill kit is available.
1. Immediately assess amount spilled, follow posted ASU Emergency Response Guide
procedures for hazardous materials incidents.
2. If a chemical exposure has occurred, a fellow lab worker shall call 9-1-1 and EH&S at (480)
965-1823.
3. Don compatible gloves and other protective PPE if not already being worn.
ASU Department of Environmental Health & Safety
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Hydrogen peroxide solutions, CAS # 7722-84-1
4. Secure / restrict access to the area of the spill to prevent spread of the chemical.
5. Use the available spill kit to stop and contain the spill. Bag the collected material.
6. Label and tag as hazardous waste and submit a pick-up request to EH&S using EHS Assistant.
Decontamination / Waste Disposal Procedure
Wearing proper PPE, decontaminate equipment and bench tops using soap and water. Rinse used
containers thoroughly with water before disposal. Dispose of hazardous waste and contaminants
following the general hazardous waste disposal guidelines below.
Label waste
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Attach a completed ASU Hazardous Waste tag to all waste containers as soon as the first
drop of waste is added to the container.
Store waste
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Store hazardous waste in closed containers, in secondary containment and in a designated
storage location.
Double-bag dry waste using sealable transparent bags.
Waste must be under the control of the person generating and disposing of it.
Dispose of waste
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Dispose of regularly generated chemical waste within 90 days.
Use EHS Assistant online hazardous waste pick-up request system.
Contact ASU EH&S at (480) 965-1823 with questions.
Protocol / Procedure
Laboratory-specific procedures
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IMPORTANT NOTE: Any deviation from this SOP requires advance PI approval.
Documentation of Training
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Prior to conducting any work with this material, Principal Investigator or designee must
provide to his/her laboratory personnel specific to the hazards involved in working with this
substance, work area decontamination, and emergency procedures.
The Principal Investigator must provide his/her laboratory personnel with a copy of this SOP
and a copy of the MSDS provided by the manufacturer.
The Principal Investigator must ensure that his/her laboratory personnel have attended
appropriate/required laboratory safety training or refresher training within the last one year.
I have read and understand the content of this SOP.
Employee Name
ASU Affiliate No.
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ASU Department of Environmental Health & Safety
Signature
Date
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Hydrogen peroxide solutions, CAS # 7722-84-1
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