TABLE S1-1: KSHV infection of endothelial cells alters global host cell metabolism.
SUPER
PATHWAY
SUB
PATHWAY
Glycine, serine
and threonine
metabolism
Alanine and
aspartate
metabolism
Glutamate
metabolism
Histidine
Lysine
metabolism
Amino Acid
Phenylalanine
& tyrosine
metabolism
Tryptophan
metabolism
Valine, leucine
and isoleucine
metabolism
Cysteine,
methionine,
SAM, taurine
metabolism
Urea cycle;
arginine-,
proline-,
metabolism
glycine
serine
N-acetylserine
threonine
alanine
beta-alanine
N-acetylalanine
aspartate
asparagine
glutamate
glutamine
pyroglutamine*
gamma-aminobutyrate
(GABA)
N-acetylglutamate
histidine
cadaverine
lysine
2-aminoadipate
N6-acetyllysine
phenylalanine
GC/MS
GC/MS
GC/MS
GC/MS
GC/MS
GC/MS
LC/MS neg
GC/MS
GC/MS
LC/MS pos
LC/MS pos
LC/MS pos
48hpi
(KSHV
vs
Mock)
1.37
1.20
1.08
1.19
1.27
1.21
1.02
0.81
1.75
1.10
2.23
0.99
GC/MS
1.21
0.97
LC/MS pos
GC/MS
GC/MS
GC/MS
GC/MS
LC/MS pos
LC/MS pos
0.29
1.07
2.67
1.33
1.16
1.11
1.14
0.34
0.92
1.59
1.18
0.86
1.32
1.13
tyrosine
LC/MS pos
1.15
1.21
kynurenine
tryptophan
C-glycosyltryptophan*
isoleucine
leucine
valine
cysteine
hypotaurine
S-adenosylhomocysteine
methionine
N-acetylmethionine
homocysteine
dimethylarginine (SDMA
+ ADMA)
arginine
ornithine
proline
citrulline
trans-4-hydroxyproline
LC/MS pos
LC/MS pos
LC/MS pos
LC/MS pos
LC/MS pos
LC/MS pos
GC/MS
GC/MS
LC/MS neg
LC/MS pos
LC/MS neg
GC/MS
6.11
1.13
1.35
1.21
1.29
1.23
1.14
1.18
1.41
1.23
1.08
0.75
31.17
1.03
1.55
1.17
1.21
1.14
0.55
0.97
1.34
1.20
0.93
0.73
LC/MS pos
1.74
1.78
LC/MS pos
GC/MS
LC/MS pos
LC/MS pos
GC/MS
1.34
1.41
1.29
2.51
1.68
1.53
1.46
1.18
2.45
1.04
BIOCHEMICAL NAME
PLATFORM
96hpi
(KSHV
vs
Mock)
1.08
1.00
0.77
0.98
1.12
0.98
0.69
0.75
1.48
1.18
2.71
1.07
Heat map of statistically significant biochemicals profiled in this study. For paired comparisons, shaded cells
indicate p≤0.05 (red indicates that the mean values are significantly higher for that comparison; green values
significantly lower). Blue-bolded text indicates 0.05< p< 0.10.
TABLE S1-2: KSHV infection of endothelial cells alters global host cell metabolism.
SUPER
PATHWAY
SUB
PATHWAY
Creatine
Polyamine
metabolism
Amino Acid
Glutathione
metabolism
Dipeptide
Peptide
Carbohydrate
gammaglutamyl
Aminosugars
metabolism
Fructose,
mannose,
galactose,
starch and
sucrose
metabolism
BIOCHEMICAL NAME
PLATFORM
creatine
5-methylthioadenosine
(MTA)
putrescine
agmatine
spermidine
spermine
glutathione, reduced
(GSH)
5-oxoproline
glutathione, oxidized
(GSSG)
cysteine-glutathione
disulfide
glycylglycine
glycylserine
glycylproline
glycylleucine
glycylglutamate
aspartylphenylalanine
cysteinylglycine
gammaglutamylglutamate
erythronate*
N-acetylneuraminate
fructose
lactose
mannose-6-phosphate
LC/MS pos
48hpi
(KSHV
vs
Mock)
1.06
LC/MS pos
0.71
0.90
GC/MS
GC/MS
LC/MS pos
GC/MS
2.76
1.53
2.66
2.94
2.48
1.33
2.97
5.96
LC/MS pos
1.20
0.98
LC/MS neg
1.15
1.10
LC/MS pos
1.21
1.19
LC/MS pos
1.25
1.06
GC/MS
GC/MS
LC/MS pos
LC/MS pos
GC/MS
LC/MS pos
GC/MS
1.34
0.98
0.80
1.09
1.05
1.19
0.89
1.11
0.89
1.02
1.19
0.90
1.22
0.73
LC/MS pos
1.22
1.40
GC/MS
GC/MS
GC/MS
GC/MS
GC/MS
0.64
0.92
1.06
0.85
0.75
0.62
1.02
1.13
0.65
3.18
GC/MS
0.43
0.28
sorbitol
96hpi
(KSHV
vs
Mock)
1.00
Heat map of statistically significant biochemicals profiled in this study. For paired comparisons, shaded cells
indicate p≤0.05 (red indicates that the mean values are significantly higher for that comparison; green values
significantly lower). Blue-bolded text indicates 0.05< p< 0.10.
TABLE S1-3: KSHV infection of endothelial cells alters global host cell metabolism.
SUPER
PATHWAY
SUB
PATHWAY
Glycolysis,
gluconeogenesi
s, pyruvate
metabolism
Carbohydrate
Nucleotide
sugars, pentose
metabolism
Krebs cycle
Energy
Oxidative
phosphorylation
BIOCHEMICAL NAME
PLATFORM
glycerate
glucose-6-phosphate
(G6P)
glucose 1-phosphate
glucose
fructose-6-phosphate
fructose 1-phosphate
Isobar: fructose 1,6diphosphate, glucose
1,6-diphosphate
2-phosphoglycerate
3-phosphoglycerate
dihydroxyacetone
phosphate (DHAP)
phosphoenolpyruvate
(PEP)
pyruvate
lactate
6-phosphogluconate
ribitol
sedoheptulose-7phosphate
gluconate
ribose
ribose 5-phosphate
Isobar: ribulose 5phosphate, xylulose 5phosphate
UDP-glucuronate
citrate
succinate
fumarate
malate
acetylphosphate
phosphate
pyrophosphate (PPi)
GC/MS
48hpi
(KSHV
vs
Mock)
1.55
96hpi
(KSHV
vs
Mock)
1.85
GC/MS
0.64
3.16
GC/MS
GC/MS
GC/MS
GC/MS
1.20
1.38
1.04
1.59
0.33
10.53
2.50
0.93
LC/MS neg
1.21
1.12
GC/MS
GC/MS
1.91
1.89
2.05
2.34
GC/MS
1.64
1.50
GC/MS
2.90
4.31
GC/MS
GC/MS
LC/MS neg
GC/MS
1.59
1.18
1.42
0.79
0.98
0.75
3.24
0.73
GC/MS
1.29
1.11
GC/MS
GC/MS
GC/MS
0.57
1.19
1.65
1.04
1.04
1.09
GC/MS
1.79
1.39
GC/MS
GC/MS
GC/MS
GC/MS
GC/MS
GC/MS
GC/MS
GC/MS
1.05
1.00
1.00
1.27
0.97
1.12
1.22
1.48
1.16
1.12
0.86
1.25
0.93
1.28
1.23
1.17
Heat map of statistically significant biochemicals profiled in this study. For paired comparisons, shaded cells
indicate p≤0.05 (red indicates that the mean values are significantly higher for that comparison; green values
significantly lower). Blue-bolded text indicates 0.05< p< 0.10.
TABLE S1-4: KSHV infection of endothelial cells alters global host cell metabolism.
SUPER
PATHWAY
SUB
PATHWAY
Essential fatty
acid
Lipid
Long chain fatty
acid
Fatty acid, ester
Fatty acid,
monohydroxy
Fatty acid,
dicarboxylate
Fatty acid,
amide
PLATFORM
48hpi
(KSHV
vs
Mock)
96hpi
(KSHV
vs
Mock)
LC/MS neg
1.99
3.14
LC/MS neg
1.68
2.63
LC/MS neg
2.15
2.80
LC/MS neg
1.99
2.28
GC/MS
GC/MS
GC/MS
GC/MS
1.34
1.27
0.98
1.26
1.37
1.38
1.12
1.50
GC/MS
1.32
1.02
GC/MS
LC/MS neg
GC/MS
LC/MS neg
1.18
1.57
1.08
1.53
1.31
1.63
1.41
1.58
LC/MS neg
1.55
1.46
GC/MS
1.18
1.57
LC/MS neg
1.67
1.43
LC/MS neg
1.70
1.65
LC/MS neg
LC/MS neg
GC/MS
LC/MS neg
LC/MS neg
LC/MS neg
GC/MS
GC/MS
GC/MS
GC/MS
LC/MS neg
LC/MS neg
1.38
1.65
1.14
1.65
1.83
1.36
1.29
0.69
0.89
1.08
1.77
1.46
1.23
2.32
1.27
1.22
2.18
2.02
0.99
0.72
1.14
0.48
3.01
2.50
2-hydroxyglutarate
GC/MS
1.12
1.04
oleamide
GC/MS
1.69
1.71
BIOCHEMICAL NAME
dihomo-linolenate
(20:3n3 or n6)
eicosapentaenoate
(EPA; 20:5n3)
docosapentaenoate (n3
DPA; 22:5n3)
docosahexaenoate
(DHA; 22:6n3)
myristate (14:0)
palmitate (16:0)
palmitoleate (16:1n7)
margarate (17:0)
10-heptadecenoate
(17:1n7)
stearate (18:0)
oleate (18:1n9)
cis-vaccenate (18:1n7)
linoleate (18:2n6)
10-nonadecenoate
(19:1n9)
arachidate (20:0)
eicosenoate (20:1n9 or
11)
dihomo-linoleate
(20:2n6)
mead acid (20:3n9)
arachidonate (20:4n6)
behenate (22:0)
docosadienoate (22:2n6)
docosatrienoate (22:3n3)
adrenate (22:4n6)
lignocerate (24:0)
hexacosanoate
n-Butyl Oleate
4-hydroxybutyrate (GHB)
2-hydroxystearate
2-hydroxypalmitate
Heat map of statistically significant biochemicals profiled in this study. For paired comparisons, shaded cells
indicate p≤0.05 (red indicates that the mean values are significantly higher for that comparison; green values
significantly lower). Blue-bolded text indicates 0.05< p< 0.10.
TABLE S1-5: KSHV infection of endothelial cells alters global host cell metabolism.
SUPER
PATHWAY
SUB
PATHWAY
Glycerolipid
metabolism
Inositol
metabolism
Lipid
Lysolipid
BIOCHEMICAL NAME
PLATFORM
choline phosphate
ethanolamine
phosphoethanolamine
choline
glycerol 3-phosphate
(G3P)
glycerophosphorylcholine
(GPC)
cytidine 5'diphosphocholine
myo-inositol
inositol 1-phosphate
(I1P)
scyllo-inositol
1palmitoylglycerophospho
ethanolamine
2palmitoylglycerophospho
ethanolamine*
2palmitoleoylglycerophosp
hoethanolamine*
1stearoylglycerophosphoe
thanolamine
2oleoylglycerophosphoeth
anolamine*
1arachidonoylglycerophos
phoethanolamine*
2arachidonoylglycerophos
phoethanolamine*
2docosapentaenoylglycer
ophosphoethanolamine*
2docosahexaenoylglycero
phosphoethanolamine*
LC/MS pos
GC/MS
GC/MS
LC/MS pos
48hpi
(KSHV
vs
Mock)
1.39
1.28
1.03
1.23
96hpi
(KSHV
vs
Mock)
1.40
1.06
0.98
1.15
GC/MS
0.54
0.36
LC/MS pos
0.44
0.31
LC/MS pos
1.14
1.06
GC/MS
0.26
1.32
GC/MS
1.23
1.74
GC/MS
1.04
1.19
LC/MS pos
0.95
0.87
LC/MS pos
0.67
0.64
LC/MS pos
0.49
0.48
LC/MS neg
1.38
1.14
LC/MS pos
0.63
0.47
LC/MS neg
1.44
1.81
LC/MS pos
0.65
0.62
LC/MS pos
0.73
0.67
LC/MS pos
0.66
0.53
Heat map of statistically significant biochemicals profiled in this study. For paired comparisons, shaded cells
indicate p≤0.05 (red indicates that the mean values are significantly higher for that comparison; green values
significantly lower). Blue-bolded text indicates 0.05< p< 0.10.
TABLE S1-6: KSHV infection of endothelial cells alters global host cell metabolism.
SUPER
PATHWAY
SUB
PATHWAY
Lysolipid
Lipid
Monoacylglycer
ol
BIOCHEMICAL NAME
1myristoylglycerophospho
choline
2myristoylglycerophospho
choline*
1palmitoylglycerophospho
choline
2palmitoylglycerophospho
choline*
1palmitoleoylglycerophosp
hocholine*
2palmitoleoylglycerophosp
hocholine*
1stearoylglycerophosphoc
holine
1oleoylglycerophosphocho
line
2oleoylglycerophosphocho
line*
2arachidonoylglycerophos
phocholine*
2docosapentaenoylglycer
ophosphocholine*
1stearoylglycerophosphoi
nositol
1arachidonoylglycerophos
phoinositol*
1-palmitoylglycerol (1monopalmitin)
1-stearoylglycerol (1monostearin)
PLATFORM
48hpi
(KSHV
vs
Mock)
96hpi
(KSHV
vs
Mock)
LC/MS pos
0.65
1.06
LC/MS pos
0.97
1.28
LC/MS pos
0.71
0.95
LC/MS pos
0.55
0.94
LC/MS pos
0.59
1.18
LC/MS pos
0.92
0.91
LC/MS pos
0.77
1.02
LC/MS pos
0.73
1.09
LC/MS pos
0.70
0.87
LC/MS pos
0.70
0.84
LC/MS pos
0.87
0.86
LC/MS neg
2.60
4.94
LC/MS neg
1.01
2.56
GC/MS
1.05
1.26
GC/MS
1.13
1.43
Heat map of statistically significant biochemicals profiled in this study. For paired comparisons, shaded cells
indicate p≤0.05 (red indicates that the mean values are significantly higher for that comparison; green values
significantly lower). Blue-bolded text indicates 0.05< p< 0.10.
TABLE S1-7: KSHV infection of endothelial cells alters global host cell metabolism.
SUPER
PATHWAY
SUB
PATHWAY
Diacylglycerol
Sphingolipid
Lipid
Sterol/Steroid
Purine
metabolism,
(hypo)xanthine /
inosine
containing
Purine
metabolism,
adenine
containing
Nucleotide
Purine
metabolism,
guanine
containing
Purine
metabolism,
urate
metabolism
Pyrimidine
metabolism,
cytidine
containing
Pyrimidine
metabolism,
orotate
containing
Pyrimidine
metabolism,
uracil
containing
Purine and
pyrimidine
metabolism
BIOCHEMICAL NAME
PLATFORM
1,2-dipalmitoylglycerol
sphinganine
sphingosine
cholesterol
7-dehydrocholesterol
7-betahydroxycholesterol
xanthine
hypoxanthine
GC/MS
LC/MS pos
LC/MS pos
GC/MS
GC/MS
48hpi
(KSHV
vs
Mock)
0.57
0.95
1.01
0.85
0.65
96hpi
(KSHV
vs
Mock)
0.99
1.04
1.20
1.00
0.90
GC/MS
1.01
1.82
GC/MS
LC/MS neg
1.51
1.39
1.70
1.42
inosine
LC/MS neg
1.29
0.75
adenine
adenosine
adenosine 5'monophosphate (AMP)
adenosine 5'diphosphate (ADP)
guanine
guanosine
guanosine 5'monophosphate (GMP)
GC/MS
LC/MS neg
0.87
0.21
0.84
0.24
LC/MS pos
0.56
0.25
LC/MS neg
0.40
0.31
GC/MS
LC/MS neg
3.02
1.11
4.69
0.69
LC/MS pos
0.69
0.89
allantoin
GC/MS
0.79
0.99
cytidine 5'monophosphate (5'CMP)
LC/MS pos
1.37
0.91
orotate
GC/MS
0.56
0.97
uracil
uridine
uridine 5'monophosphate (UMP)
GC/MS
LC/MS neg
2.59
1.70
2.57
2.21
LC/MS pos
0.81
0.75
methylphosphate
GC/MS
0.92
1.06
Heat map of statistically significant biochemicals profiled in this study. For paired comparisons, shaded cells
indicate p≤0.05 (red indicates that the mean values are significantly higher for that comparison; green values
significantly lower). Blue-bolded text indicates 0.05< p< 0.10.
TABLE S1-8: KSHV infection of endothelial cells alters global host cell metabolism.
SUPER
PATHWAY
SUB
PATHWAY
Ascorbate and
aldarate
metabolism
Folate
metabolism
Cofactors
and
vitamins
Nicotinate and
nicotinamide
metabolism
Pantothenate
and CoA
metabolism
Riboflavin
metabolism
Tocopherol
metabolism
Chemical
Xenobiotics
Sugar, sugar
substitute,
starch
ascorbate (Vitamin C)
GC/MS
48hpi
(KSHV
vs
Mock)
0.38
threonate
GC/MS
0.40
0.51
LC/MS neg
0.88
0.82
LC/MS pos
1.79
1.10
LC/MS pos
0.72
1.05
LC/MS neg
0.65
0.75
LC/MS pos
1.14
1.21
LC/MS pos
LC/MS pos
1.59
0.78
1.83
0.94
coenzyme A
LC/MS neg
1.50
1.46
flavin adenine
dinucleotide (FAD)
LC/MS neg
0.98
1.04
GC/MS
0.86
0.86
GC/MS
LC/MS neg
0.65
2.10
0.34
2.08
GC/MS
1.09
0.94
BIOCHEMICAL NAME
5-methyltetrahydrofolate
(5MeTHF)
nicotinamide
nicotinamide adenine
dinucleotide (NAD+)
nicotinamide adenine
dinucleotide reduced
(NADH)
nicotinamide adenine
dinucleotide phosphate
(NADP+)
1-methylnicotinamide
pantothenate
alpha-tocopherol
glycerol 2-phosphate
phenol red
erythritol
PLATFORM
96hpi
(KSHV
vs
Mock)
0.18
Heat map of statistically significant biochemicals profiled in this study. For paired comparisons, shaded cells
indicate p≤0.05 (red indicates that the mean values are significantly higher for that comparison; green values
significantly lower). Blue-bolded text indicates 0.05< p< 0.10.