SUPPLEMENTARY DATA
TABLES
Supplementary Table 1. Correlational analysis of factors involved in adhesion and pathogen inhibition using Spearman’s correlational analysis.
Supplementary Table 2. List of adhesion-relation homologs in L. mucosae LM1
FIGURES
Supplementary Figure 1. Beta-galactosidase activity of the selected Lactobacilli strains
Supplementary Table 1. Correlational analysis of factors involved in adhesion and pathogen inhibition using Spearman’s correlational analysis.
Correlations
HYDROPHOBICITY AGGREGATION
AGGREGATION
E. COLI
Spearman's rho
S. TYPHIMURIUM
COMEX-ST
DISP-EC
DISP-ST
.410
.588
.367
-.050
-.661
-.450
Sig. (2-tailed)
.
.050
.042
.273
.096
.332
.898
.053
.224
N
9
9
9
9
9
9
9
9
9
Correlation Coefficient
.667*
1.000
.683*
.536
.773*
.150
.233
-.746*
-.300
Sig. (2-tailed)
.050
.
.042
.137
.015
.700
.546
.021
.433
9
9
9
9
9
9
9
9
9
Correlation Coefficient
.683*
.683*
1.000
.351
.437
.567
.150
-.390
-.383
Sig. (2-tailed)
.042
.042
.
.354
.240
.112
.700
.300
.308
9
9
9
9
9
9
9
9
9
Correlation Coefficient
.410
.536
.351
1.000
.903**
-.109
.460
-.868**
-.536
Sig. (2-tailed)
.273
.137
.354
.
.001
.781
.213
.002
.137
9
9
9
9
9
9
9
9
9
Correlation Coefficient
.588
.773*
.437
.903**
1.000
-.025
.370
-.974**
-.529
Sig. (2-tailed)
.096
.015
.240
.001
.
.949
.327
.000
.143
9
9
9
9
9
9
9
9
9
N
COAGGREGATION W/
COMEX-EC
.683*
N
COAGGREGATION W/
COAGG-ST
.667*
N
ADHESION
COAGG-EC
1.000
Correlation Coefficient
HYDROPHOBICITY
ADHESION
N
E. COLI INHIBITION IN
Correlation Coefficient
.367
.150
.567
-.109
-.025
1.000
-.333
-.017
-.083
COMPETITIVE
Sig. (2-tailed)
.332
.700
.112
.781
.949
.
.381
.965
.831
EXCLUSION
N
9
9
9
9
9
9
9
9
9
S. TYPHIMURIUM
Correlation Coefficient
-.050
.233
.150
.460
.370
-.333
1.000
-.237
-.100
INHIBITION IN
Sig. (2-tailed)
.898
.546
.700
.213
.327
.381
.
.539
.798
9
9
9
9
9
9
9
9
9
-.661
-.746*
-.390
-.868**
-.974**
-.017
-.237
1.000
.441
.053
.021
.300
.002
.000
.965
.539
.
.235
9
9
9
9
9
9
9
9
9
-.450
-.300
-.383
-.536
-.529
-.083
-.100
.441
1.000
.224
.433
.308
.137
.143
.831
.798
.235
.
9
9
9
9
9
9
9
9
9
COMPETITIVE
EXCLUSION
N
Correlation Coefficient
E. COLI INHIBITION IN
DISPLACEMENT
Sig. (2-tailed)
N
S. TYPHIMURIUM
Correlation Coefficient
ADHESION IN
Sig. (2-tailed)
DISPLACEMENT
N
*. Correlation is significant at the 0.05 level (2-tailed).
**. Correlation is significant at the 0.01 level (2-tailed).
Supplementary Table 2. List of adhesion-relation homologs in L. mucosae LM1
LOCATION
POTENTIAL CELL SURFACE EXPOSED
PROTEINS
GENE NUMBER
FUNCTION
GRAVY index
Mucin Binding Protein (MUB) LPXTG
LBLM1_04370
Type IV secretory pathway, TrbL components
-0.720
LBLM1_18230
Hypothetical protein
-0.262
LBLM1_01550
hypothetical protein
-0.620
LBLM1_01450
hypothetical protein
-0.333
LBLM1_01560
Type IV secretory pathway, TrbL components
-0.608
LBLM1_01570
Type IV secretory pathway, TrbL components
-0.584
LBLM1_06560
hypothetical protein
-0.517
LBLM1_00070
predicted solute binding protein
-0.362
LBLM1_07180
predicted membrane protein
-0.205
LBLM1_06380
recombination protein J, recJ
-0.293
LBLM1_15660
citrate lyase regulator
-0.025
LBLM1_07050
response regulator
-0.334
LBLM1_13300
acetyl-CoA carboxylase, biotin carboxylase
-0.195
LBLM1_04800
Predicted drug exporters of the RND superfamily
0.028
LBLM1_16150
type I site-specific deoxyribonuclease, HsdR family
0.767
LBLM1_00710
hypothetical protein
-0.292
LBLM1_01370
predicted outer membrane protein
-0.517
LBLM1_01680
RhoGEF, Guanine nucleotide exchange factor for
Rho/Rac/Cdc42-like GTPases
-0.070
LBLM1_02180
hypothetical protein
0.463
LBLM1_03400
Uncharacterized protein conserved in bacteria with the
myosin-like domain
-0.580
LBLM1_04360
hypothetical protein
-0.458
LBLM1_05330
AAA ATPase containing von Willebrand factor type A
(vWA) domain
-0.637
LBLM1_07660
5'-nucleotidase/2',3'-cyclic phosphodiesterase and related
esterases; LPXTG motif cell wall domain protein
-0.357
Mucus Binding Protein (MucBP) Domain
Containing Proteins LPXTG
Cell Wall
LPXTG domain
LBLM1_07910
hypothetical protein
-0.334
LBLM1_18030
cell division protein
-0.680
LBLM1_18390
Multidrug resistance efflux pump
-0.365
LBLM1_18950
Uncharacterized protein conserved in bacteria
-0.597
LBLM1_06040
extracellular protein, gamma-D-glutamate-mesodiaminopimelate muropeptidase; Cell wall-associated
hydrolases (invasion-associated proteins)
-0.151
LBLM1_10380
LysM domain protein
-0.405
LBLM1_10410
hypothetical protein
-0.331
LBLM1_12480
peptidoglycan-binding protein
-0.419
LBLM1_16320
Cell wall-associated hydrolases (invasion-associated
proteins)
-0.176
LBLM2_17320
mannosyl-glycoprotein endo-beta-N-acetylglucosamidase;
muramidase (flagellum-specific)
-0.280
LBLM1_19190
phage lysin; lyzozyme M1 (1,4-beta-N-acetylmuramidase)
-0.377
LBLM1_07670
glucan binding domain (YG repeat)
-0.693
LBLM1_14570
pectin methylesterase
-0.538
LBLM1_20020
hypothetical protein
-0.865
LBLM1_20030
glucan binding domain (YG repeat)
-0.767
LBLM1_18100
SecA DEAD domain protein; Preprotein translocase subunit
SecA (ATPase, RNA helicase)
-0.373
Adhesin-like protein
LBLM1_10110
ABC superfamily ATP binding cassette transporter, binding
protein
-0.406
Sortase (SrtA)
LBLM1_02950
Sortase (surface protein transpeptidase)
-0.270
LysM domains
CW-binding repeats
Membrane
(A) Lactobacillus mucosae LM1
(B) Lactobacillus johnsonii PF01
Supplementary Figure 1. Beta-galactosidase activity of the selected Lactobacilli strains
(C) Lactobacillus rhamnosus GG