Additional file 4. Selected specimens representing sampled genetic diversity and GenBank accession numbers for the six sampled loci: nuclear ribosomal internal transcribed spacer region (ITS) and large subunit (LSU), mitochondrial small subunit (mtSSU), and protein-coding makers MCM7, RPB1, and RPB2. Taxon Specimen ID Voucher # Melanohale a elegantula ITS nuLSU mtSSU MCM7 RPB1 RPB2 XX000000 XX000000 XX000000 XX000000 XX000000 XX000000 3745 3905 3958 4137 4295 4303 4307 4315 4317 4363 Essl16362 Essl16550 USA, Esslinger, 18874 (TLE) USA, Spribille 20670 (GZU) Greenland, Hansen ESH-09B.278 (TLE) USA, Leavitt 10-357 (F) USA, Leavitt 10.site10.01 USA, Leavitt 10.site12.01 USA, Leavitt 13.site13.01 USA, Leavitt 10.site18.01 (F) USA, Leavitt 10.site18.03 (F) USA, Leavitt 10.site45.01 (F) USA, Esslinger 16362 (TLE) USA, Esslinger 16550 (TLE) x x x x x x x x x x AY611120 AY611116 x x x x x x x x x AY611176 AY611172 x x x x x x x AY607834 AY607830 x x x x x x x x x x - x x x x x x x x x x - x x x x x x x x x x - 6042 Spain, 10224 (MAF) x x x x x - 3665 3683 3907 4110 4501 MAF10225 MAF10227 MAF10230 The Netherlands, Aptroot 68148 (F) Scotland, Coppins 23035 (F) Greece, Spribille 18819 (GZU) Estonia, 55230 (TU) Norway, Bjerke 5-9-2010 (TLE) Spain, 10225 (MAF) Spain, 10227 (MAF) Spain, 10230 (MAF) x x x x x AY611092 AY611082 AY611095 x x x x x AY611149 AY611139 AY611153 x x x x AY607804 AY607794 AY607808 x x x x - x x x x x x x - 6043 6044 Spain, 10214 (MAF) Spain, 7636 (MAF) x x x x x x x x x x x x 3746 USA, Esslinger 18855 (TLE) x x x x x X M. elegantulaS PAIN M. exasperata M. exasperata SPAIN M. exasperatul a 3761 3768 3775 3776 3779 3926 4116 4274 4277 4278 4312 4321 Essl16554 MAF10213 USA, Esslinger 18682 (TLE) USA, Henson 2/2010 (TLE) USA, Leavitt 09-344a (F) USA, Leavitt 09-344b (F) USA, Leavitt 09-344e (F) Sweden, Thell s.n. (LD) Estonia, 51003 (TU) USA, Leavitt 10.site32.01 (F) USA, Leavitt 10.site32.03 (F) USA, Leavitt 10.site32.04 (F) USA, Leavitt 10.site16.01 (F) USA, Leavitt 10.site23.01 (F) USA, Esslinger 16554 (TLE) 10213 (MAF) x x x x x x x x x x x x AY611119 AY611090 x x x x x x x x x x x x AY611175 AY611147 x x x x x x x x x x x AY607833 AY607802 x x x x x x x x x x x x - x x x x x x x x x x x x EF092124 X X X X X X X X X X X X - 6049 6050 India, 3929 (MAF) India, 1109 (MAF) x x x - x - x x x x x 4006 4011 USA, Harris 55554 (NY) USA, Harris 54342 (NY) x x x x x x x x x x x x 3892 3960 3961 4888 USA, Spribille 52177 (KLGO) Greenland, ESH-08.265 (TLE) Greenland, ESH-08.190 (TLE) USA, Esslinger BP78-4 (TLE) x x x x x x x x x x x x x x x - x x x x x x x x 3895 4075 4091 4093 MAF10218 MAF10226 MAF10231 Sweden, Thell s.n. (LD) Spain, Crespo 6027g (MAF) Spain, Crespo 6028h (MAF) Spain, Crespo s.n. (MAF) Spain, 10218 (MAF) Spain, 10226 (MAF) Spain, 10231 (MAF) x x x x AY611078 AY611079 AY611094 x x x x AY611135 AY611136 AY611151 x x x AY607790 AY607791 AY607806 x x x x - x x x x x - x x x x - 3647 3661 China, Wang 20084037 (SDNU) China, Wang 20084052-1 (SDNU) x x x - - x - - - M. gomukhensi s M. halei M. infumata M. laciniatula M. lobulata M. multispora1 3762 3780 4010 4299 4301 4302 Essl16555 USA, Esslinger 18757 (TLE) USA, Leavitt 10-003a (F) USA, Lendemer 11408 (NY) USA, Leavitt 10.site12.01 (F) USA, Leavitt 10.site12.03 (F) USA, Leavitt 10.site12.04 (F) USA, Esslinger 16555 (TLE) x x x x x x AY611123 x x x x x x AY611178 x x x x x x AY607837 x x x x x x - x x x x x x - x x x x x x - 4653 4837 Canada, Bjork 13320 (TLE) Canada, Esslinger BP100-1 (TLE) x x x x x x x x x x 3750 3756 3763 3909 3910 4267 4283 4344 4355 4368 4663 4684 4882 4885 USA, Esslinger 18888 (TLE) USA, Walton 11126 (TLE) USA, Esslinger 18673 (TLE) USA, Spribille 20869 (GZU) USA, Spribille 20848 (GZU) USA, Glacy s.n. (F) USA, Leavitt 10.site33.02 (F) USA, Leavitt 10.site38.03 (F) USA, Leavitt 10.site40.02 (F) USA, Leavitt 10.site46.01 (F) Canada, Goward 07-292 (TLE) Canada, Goward 07-200a (TLE) USA, Esslinger BP73-4 (TLE) USA, Esslinger BP78-1 (TLE) x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x - x x x x x x x x x x x x x x x x x x x x x x x 3662 4106 4393 4499 4624 4665 4869 MAF7666 UPS38815 4 China, Ren s.n. (SDNU) Estonia, 45196 (TU) USA, McCune 30737 (OSU) Norway, Bjerke 6-9-2010 (TLE) Japan, Y. Ohmura 7132 (F) Norway, Goward 02-1400 (TLE) USA, Esslinger BP65-2 (TLE) Spain, 7666 (MAF) Sweden, 38815 (UPS) x x x x x x x x x x x x x x x x x - x x x x x x x x x x x x - x x x x x x - x x x x x x - M. multispora2 M. multispora3 M. olivacea M. olivaceoides 1 4676 4891 Canada, Bjork 16460 (TLE) USA, Esslinger BP80-1 (TLE) x x x x x x x x x x x 4859 4895 USA, Esslinger BP50-1 (TLE) USA, Esslinger BP81-1 (TLE) x x x x x x x x x - 3644 3659 6048 China, Wang 20084048 (SDNU) China, Wang 20084093-1 (SDNU) India, Divakar 3929/A (MAF) x x x x - x x X 3682 4195 4197 4391 4392 4862 4886 Ahti60893 Scotland, Coppins 23033 (F) USA, Lumbsch 20113a (F) USA, Lumbsch 20117a (F) USA, McCune 30779 (OSU) USA, McCune 30781 (OSU) USA, Esslinger BP53-1 (TLE) USA, Esslinger BP78-2 (TLE) Finland, Ahti 60893 (H) x x x x x x x AY611093 x x x x x x x AY611150 x x x x AY607805 x x x x - x x x x x - X X X X X X - 3747 3748 3755 4813 4887 Essl16132 USA, Esslinger 18877 (TLE) USA, Walton 11095 (TLE) USA, Walton 11224 (TLE) USA, Esslinger BP117-1 (TLE) USA, Esslinger BP78-3 (TLE) USA, Esslinger 16132 (TLE) x x x x x AY611115 x x x x x AY611171 x x x AY607829 x x x - x x X X X X X - 3649 3655 China, Wang 20084074-1 (SDNU) China, Du 20084031-1 (SDNU) x x - - - - - 3964 3979 3991 USA, Leavitt 1036.FW5 (F) USA, Leavitt 1036.FW2 (F) USA, Leavitt 1036.FW6 (F) x x x x x x x x x x x x x x x X X X M. olivaceoides 2 M. poeltii M. septentriona lis M. subelegantu la M. subexasper ata M. subolivacea 1 4125 4127 4605 USA, Leavitt 10-317 (F) USA, Leavitt 10-319 (F) USA, Leavitt 11-015 (F) x x x x x x x - x x x x x x X X X 3767 3983 3988 4012 4105 4123 4124 4130 4135 4269 4296 4341 4613 4614 4636 USA, Esslinger 18832 (TLE) USA, Leavitt 10-30.RC2 (F) USA, Leavitt 10-40.KC4b (F) USA, Lendemer 14631 (NY) USA, Crespo 6009k (MAF) USA, Leavitt 10-307 (F) USA, Leavitt 10-309 (F) USA, Leavitt 10-330 (F) USA, Leavitt 10-338 (F) USA, Kellison s.n. (F) USA, Leavitt 10.site10.01 (F) USA, Leavitt 10.site37.01 (F) USA, Leavitt 11-023 (F) USA, Leavitt 11-024 (F) Canada, Goward 09-89a (TLE) x x x x x x x x x X X X X X X x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x X X X X X X X X X X X X X X - 3920 4857 4868 4876 USA, Spribille s.n. (GZU) USA, Esslinger BP47-2 (TLE) USA, Esslinger BP65-1 (TLE) USA, Esslinger BP72-1 (TLE) X X X X x x x x x x x x x x x x x X X X X 6045 Argentina, SC 1938 (MAF) X x - x x X 6046 6047 Chile, Chile 332 (MAF) Chile, Chile 337 (MAF) X X x x - x x x x X X 3742 USA, Esslinger 18915 (TLE) X x M. subolivacea 2 M. trabeculata M. ushuaiensis Argentina M. ushuaiensis Chile Melanelixia californica