SUPPLEMENTARY MATERIAL
New fatty acid, aromatic ester and monoterpenic benzyl glucoside from the fruits
of Withania coagulans Dunal
Abuzer Ali, Mohammad Jameel and Mohammed Ali*
Phytochemistry Research Laboratory, Department of Pharmacognosy and
Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi-110062, India.
*Corresponding author. Email: maliphyto@gmail.com
Abstract
The fruits of Withania coagulans Dunal (family: Solanaceae) are sweet,
sedative, emetic, alterative and diuretic; used to treat asthma, biliousness,
strangury, wounds, dyspepsia, flatulent colic, liver complaints and intestinal
infections in the indigenous system of medicine. Phyotochemical investigation
of the methanolic extract of Withania coagulans fruits led to the isolation of a
new fatty acid, an aromatic ester and a monoterpenic benzyl glucoside
characterized
as
n-octatriacont-17-enoic
acid
(3),
geranilan-10-olyl
dihydrocinnamoate (4) and geranilan-8-oic acid-10-olyl salicyloxy-2-O--Dglucofuranosyl-(6′′→1′′′)-O--D-glucofuranosyl-6′′′-n-octadec-9′′′′,11′′′′dienoate (5) along with two known fatty acids, n-dotriacont-21-enoic acid (1)
and n-tetratriacontanoic acid (2). The structures of isolated phytoconstituents
were established on the basis of 1D- and 2D-NMR, FT-IR, UV, and MS data
and chemical means.
Keywords: Withania coagulans; Solanaceae; Indian rennet; fatty acid;
aromatic ester
Botanical aspects of Withania coagulans Dunal
Withania coagulans Dunal (family: Solanaceae) is a rigid, gray undershrub, 60-120
cm. high, popularly known as Indian rennet, vegetable rennet, or Indian cheese maker.
The fruits are globose, red and brownish, smooth, enclosed in leathery calyx; pulp is
brown having nauseous and fruity odour.
Figure S1. HMBC spectrum of n-dotriacont-21-enoic acid (1)
Figure S2. HMBC spectrum of n-tetratriacontanoic acid (2)
Figure S3. HMBC spectrum of n-octatriacont-17-enoic acid (3)
Figure S4. HMBC spectrum of geranilan-10-olyl dihydrocinnamoate (4)
Figure S5. HMBC spectrum of geranilanolyl salicylic glycoside (5)
Figure S6. HSQC spectrum of n-dotriacont-21-enoic acid (1)
Figure S7. HSQC spectrum of n-tetratriacontanoic acid (2)
Figure S8. HSQC spectrum of n-octatriacont-17-enoic acid (3)
Figure S9. HSQC spectrum of geranilan-10-olyl dihydrocinnamoate (4)
Figure S10. HSQC spectrum of geranilanolyl salicylic glycoside (5)
Figure S11. HRMS spectrum of n-dotriacont-21-enoic acid (1)
Figure S12. HRMS spectrum of n-tetratriacontanoic acid (2)
Figure S13. HRMS spectrum of n-octatriacont-17-enoic acid (3)
Figure S14. HRMS spectrum of geranilan-10-olyl dihydrocinnamoate (4)
Figure S15. HRMS spectrum of geranilanolyl salicylic glycoside (5)