GENBANK ACCESSION NUMBER
CVU55761
CLONE
pEGFP-1
BOX
GS4, DS2, TS1
GLYCEROL STOCK NUMBER
258A, 258B, 258C
-SENSE DIGEST
N/A
DROP-OUT DIGEST
N/A
PLASMID
pEGFP-1 (4.2 kb)
INSERT SIZE
N/A
RESISTANCE
kanamycin
STRAIN
DH5-α
REFERENCE
Clontech, discontinued
INFO
Expression vector with MCS upstream of GFP. Does not contain a promoter.
GLYCEROL STOCK GENERATED BY: Rebecca Bernardos
GENBANK ACCESSION NUMBER
PLASMID NAME
gfap Intron1 (zebrafish)
STOCK #
265A, B, C
LOCATION (BOXES)
GS4, DS3, TS2
-SENSE DIGEST & TRANSCRIPTION
N/A
DROP-OUT DIGEST
EcoRI
VECTOR NAME & SIZE
pCR-BluntII-TOPO (3519 bp)
INSERT SIZE
4391 bp
ANTIBIOTIC RESISTANCE
Kanamycin
HOST CELL STRAIN
DH5-T1
OTHER INFO/REFERENCES:
Made by RLB:
Genomic DNA cloned from BAC ZK15N1 (#244).
PCR primers (used High Fidelity Taq kit):
Fwd 5’ gaa t ctc gag gta agg act gag gtg atg 3’
extra XhoI
Rev 5’ ggc c gaa ttc aga tgg ata agg aaa cag 3’
extra EcoRI
See the following for vector details:
Bernardos, R.L. & Raymond, P.A. (2006). GFAP transgenic zebrafish. Gene Expression Patterns 6:1007-1013.
GENBANK ACCESSION NUMBER
PLASMID NAME
gfap Intron1:EGFP1
STOCK #
266A, B, C
LOCATION (BOXES)
GS4, DS3, TS2
-SENSE DIGEST & TRANSCRIPTION
N/A
DROP-OUT DIGEST
XhoI & EcoRI
VECTOR NAME & SIZE
pEGFP-1 (4.2 kb)
INSERT SIZE
4391 bp
ANTIBIOTIC RESISTANCE
Kanamycin
HOST CELL STRAIN
DH5-
OTHER INFO/REFERENCES:
Made by RLB:
gfap Intron1 dropped out of pCR-BluntII-TOPO vector (#265) by XhoI & EcoRI cuts. Ligated into XhoI and EcoRI sites
in pEGFP-1 (#258).
See the following for vector details:
Bernardos, R.L. & Raymond, P.A. (2006). GFAP transgenic zebrafish. Gene Expression Patterns 6:1007-1013.
GENBANK ACCESSION NUMBER
From BAC ZK15N1
PLASMID NAME
gfap 5’/Exon1 (zebrafish)
STOCK #
264A, B, C
LOCATION (BOXES)
GS4, DS3, TS1
-SENSE DIGEST & TRANSCRIPTION
N/A
DROP-OUT DIGEST
EcoRI
VECTOR NAME & SIZE
pCR-BluntII-TOPO (3519 bp)
INSERT SIZE
3032 bp
ANTIBIOTIC RESISTANCE
Kanamycin
HOST CELL STRAIN
DH5-T1
OTHER INFO/REFERENCES:
Made by RLB:
Genomic DNA cloned from BAC ZK15N1 (#244).
PCR primers (used High Fidelity Taq kit):
Fwd 5’ gcg gc gaa ttc cac ctt tgg gat gta gtc 3’
extra
EcoRI
Rev 5’ gta a gtc gac gag gaa cgc tgg gac tcc a 3’
extra SalI
See the following for vector details:
Bernardos, R.L. & Raymond, P.A. (2006). GFAP transgenic zebrafish. Gene Expression Patterns 6:1007-1013.
GENBANK ACCESSION NUMBER
N/A
PLASMID NAME
gfap Intron1-5’/Ex1: EGFP1 (gfap:GFP)
STOCK #
267A, B, C
LOCATION (BOXES)
GS4, DS3, TS2
-SENSE DIGEST & TRANSCRIPTION
N/A
DROP-OUT DIGEST
EcoRI & SalI
VECTOR NAME & SIZE
gfap Intron1:EGFP1 (8520 bp)
INSERT SIZE
3032 bp (gfap 5’/Ex1)
ANTIBIOTIC RESISTANCE
Kanamycin
HOST CELL STRAIN
DH5-
OTHER INFO/REFERENCES:
Made by RLB:
gfap 5’/Ex1 dropped out from pCR-BluntII-TOPO (#264) by EcoRI & SalI cut, ligated into the SalI & EcoRI sites of
gfap Intron1:EGFP1 (#266).
***This vector was used to make the Tg(gfap:GFP)mi2001, mi2002, mi2003 lines.
Linearize the vector with XhoI & clean before injecting (30ng/L).
See the following for vector details:
Bernardos, R.L. & Raymond, P.A. (2006). GFAP transgenic zebrafish. Gene Expression Patterns 6:1007-1013.