OIE Collaborating Centre Reports
Activities in 2013
Title of Collaborating Centre:
Address of Collaborating Centre:
Surveillance and Control of Animal
Protozoan Diseases
National Research Center for Protozoan Diseases, Obihiro
University of Agriculture and Veterinary Medicine, Nishi
2-13, Inada-cho
Obihiro, Hokkaido 080-8555,
JAPAN
Tel.:
+81-155-49-5641
Fax:
+81-155-49-5643
e-mail address:
website:
Name of Director of Institute
(Responsible Official):
Name (including Title and
Position) of Head of the
Collaborating Centre (formally
OIE Contact Point):
protozoa@obihiro.ac.jp
http://www.obihiro.ac.jp/~protozoa
Prof. Hiroshi Suzuki, Ph.D.
Prof. Ikuo Igarashi, D.V.M., Ph.D.
Name (including Title and
Position) of writer of this report
(if different from above)
Annual reports of OIE Reference Centres, 2013
1
Surveillance and Control of Animal Protozoan Diseases
Summary of activities specifically related to the mandate of
OIE Collaborating Centres
ToR:
To provide services to the OIE, in particular within the region, in the designated
specialty, in support of the implementation of OIE policies and, where required, seek for
collaboration with OIE Reference Laboratories
ToR:
To identify and maintain existing expertise, in particular within its region
1.
Activities as a centre of research, expertise, standardisation and dissemination of
techniques within the remit of the mandate given by the OIE
Disease control
Epidemiology, surveillance, risk assessment, modelling
Training, capacity building
Zoonoses
Wildlife
Avian diseases
Aquatic animal diseases
Animal welfare
Diagnosis, biotechnology and laboratory
Veterinary medicinal products
Vaccines
Food safety
Feed safety
Other (Name the category:
)
EPIDEMIOLOGY, SURVEILLANCE, RISK ASSESSMENT, MODELING
Title of activity
Scope
Molecular
epidemiological survey
of animal African
trypanosomes
A total of 484 water buffaloes in Vietnam and of 231 of sheep, goats and
cattle sera were analysed for the presence of anti-trypanosoma antibody by
CATT/T. evansi test and Trypanosome crude antigen ELISA
Molecular detection and
characterization of
Babesia and Theileria
A total of 1824 cattle sera from Thailand were analysed for the presence of
merozoite surface antigen 2b (MSA-2b) of Babesia bovis by PCR and
phylogenic analysis was conducted. A total of 244 cattle sera from Thailand,
Brazil and Ghana were analysed for the presence of MSA-1 of Babesia bovis
by PCR and DNA sequences were conducted. A total of 71 cattle sera from
Sri Lanka were analysed for the presence of major piroplasm surface protein
(MPSP) by PCR and DNA sequences were conducted.
Prevalence and genetic
diversity of equine
piroplasms in Mongolia
A total of 250 horse sera from Mongolia were analysed for the presence of
antibodies against B. caballi and T. equi ELISA by PCR. DNA sequences of BC
48 and EMA-1 as well as phylogenic analyses have done
TRAINING, CAPACITY BUIDLING
2
Title of activity
Scope
Advanced Research
Course on International
Animal Health
This training course was financed by Japan International Cooperation
Agency (JICA). Six young scientists from Indonesia, Mongolia, Thailand,
Uganda and Zambia have been trained for basic molecular biology and
molecular diagnostics for 10 months (Oct. 2012-Aug. 2012). Another two
scientists from Indonesia and Vietnam are being trained for basic molecular
Annual reports of OIE Reference Centres, 2013
Surveillance and Control of Animal Protozoan Diseases
biology and molecular diagnostics for 10 months (Oct. 2013-Aug. 2014).
Capacity building of
graduate students and
postdoctoral fellows
Thirty-eight foreign researchers including graduate students and
postdoctoral fellows (Afghanistan, Benin, Bolivia, Burkina Faso, China,
Egypt, Kenya, Mongolia, Philippines, Sri Lanka, Thailand and Vietnam) and
51 Japanese postdoctoral fellows, graduate and undergraduate students
have been worked on protozoan diseases
DIAGNOSIS, BIOTECHNOLOGY AND LABORATORY
Title of activity
Scope
Development of a
immunechromatographi
c test (ICT) for surra,
The rapid and simple serological test (ICT) is under development using
several recombinant antigens in collaboration with Dr. Quoc Doanh
Nguyen, National Institute for Veterinary Research, Hannoi, Vietnam
ZOONOSES
Title of activity
Scope
Development of a
molecular and
serological diagnostic
test for human
babesiosis
The ICT and LAMP are under development of is under development in
collaboration with Dr. Peter Krause, Yale University, New Haven, USA
VACCINES
Title of activity
Scope
Development of a nextgeneration vaccines,
The technology in this study is novel-type of vaccine in which the vaccine
components are encapsulated lipids and oligosaccharide for Neospora
caninum infection in cattle.
ToR:
To propose or develop methods and procedures that facilitate harmonisation of
international standards and guidelines applicable to the designated specialty
2.
Proposal or development of any procedure that will facilitate harmonisation of
international regulations applicable to the surveillance and control of animal diseases,
food safety or animal welfare
Proposal title
Proposal of ringtrial for equine
piroplasmosis
Scope/Content
This proposan is planing to make
gold standard reference test for
equine piroplasmosis with
Department of Agriculture,
Fisheries and Forestry, Australia,
Animal Health and Veterinary
Laboratories Agency, UK.
Applicable area
Surveillance and control of animal diseases
Food safety
Animal welfare
ToR:
To establish and maintain a network with other OIE Collaborating Centres designated
for the same specialty, and should the need arise, with Collaborating Centres in other
disciplines
ToR:
To carry out and/or coordinate scientific and technical studies in collaboration with
other centres, laboratories or organisations
Annual reports of OIE Reference Centres, 2013
3
Surveillance and Control of Animal Protozoan Diseases
3.
Did your Collaborating Centre maintain a network with other OIE Collaborating Centres
(CC), Reference Laboratories (RL), or organisations designated for the same specialty, to
coordinate scientific and technical studies?
Yes
Name of OIE CC/RL/other
organisation(s)
4.
No
Location
Region of
networking Centre
Purpose
Surra;Group on Non Tsetse
Transmitted Animal
Trypanosomoses (NTTAT)
(Chairperson: Dr. Louis
Touratier), Prof. Philippe
Büscher Institute of
Tropical Medicine Antwerp
Paris,
Antwerp
Africa
Americas
Asia and Pacific
Europe
Middle East
This network was formed to control
Non Tsetse Transmitted Animal
Trypanosomoses (NTTAT) and meeting
was hels to exchange latest information
every year.
Equine piroplamosis: Animal
Diseases Research Unit
(USDA/ARS), Canadian Food
Inspection Agency, National
Research Institute of Equids
(India)
USA,
Canada,
India
Africa
Americas
Asia and Pacific
Europe
Middle East
This network was formed to exchnage
lates information on equine
piroplamosis for update of OIE anula
and supplu of diadnostic materilas.
Did your Collaborating Centre maintain a network with other OIE Collaborating Centres,
Reference laboratories, or organisations in other disciplines, to coordinate scientific and
technical studies?
Yes
No
Name of OIE CC/RL/other
organisation(s)
Location
Onderstepoort Veterinary
Institute
Onderste
poort
Region of
networking Centre
Africa
Americas
Asia and Pacific
Europe
Middle East
Purpose
This network was maintained to do
inter-lab comparison / proficiency
testing with our research center for
quality purposes in bovine babeiosis
and Ehrlichia ruminantium
ToR:
To place expert consultants at the disposal of the OIE.
5.
Did your Collaborating Centre place expert consultants at the disposal of the OIE?
Yes
4
No
Name of expert
Kind of consultancy
Subject
Dr. Noboru Unoue
Co-athor of the chapter
Co-author of Chapter (surra) in OIE Manuals
of Diagnostic Tests and Vaccine Development
for Terrestrial Animals
Dr. Ikuo Igarashi
Co-athor of the chapter
Co-author of Chapters (bovine babeiosis and
equine piropmamosis) in OIE Manuals of
Diagnostic Tests and Vaccine Development
for Terrestrial Animals
Annual reports of OIE Reference Centres, 2013
Surveillance and Control of Animal Protozoan Diseases
ToR:
To provide, within the designated specialty, scientific and technical training to personnel
from OIE Member Countries
6.
Did your Collaborating Centre provide scientific and technical training, within the remit of
the mandate given by the OIE, to personnel from OIE Member Countries?
Yes
No
If the answer is yes, please provide the total number of trained persons for each of the following
categories:
a)
Technical visits: ................................................................ 1
b)
Seminars: ......................................................................... 12
c)
Hands-on training courses: .............................................. 0
d)
Internships (>1 month): ................................................... 2
Type of technical
training provided
(a, b, c or d)
Content
Country of origin of the
expert(s) provided with
training
No. participants from
the corresponding
country
a
Provided training on IFAT and ELISA
of equine piroplasmosis
South Korea
1
d
Provided training on culture, drug
screenings,IFAT, ELISA, PCR of
bovine piropamosis
Egypt
5
b
Provided 12 seminars on babesiosis,
trypanosomosis, toxoplasmosis,
malaria and tick-borne diseases
Afghanistan, Benin,
Bolivia, Burkina Faso,
China, Egypt, Kenya,
Mongolia, Philippines, Sri
Lanka, Thailand and
Vietnam
30
ToR:
To organise and participate in scientific meetings and other activities on behalf of the
OIE
7.
Did your Collaborating Centre organise or participate in the organisation of scientific
meetings on behalf of the OIE?
Yes
National/
International
International
No
Title of event
Co-organiser
Date
(mm/yy)
Location
No.
Participants
Meeting of the Group on
Non Tsetse Transmitted
Animal Trypanosomoses
(NTTAT)
OIE
May 2013
Paris
30
ToR:
To collect, process, analyse, publish and disseminate data and information relevant to
the designated specialty
8.
Publication and dissemination of any information within the remit of the mandate given by
the OIE that may be useful to Member Countries of the OIE
Annual reports of OIE Reference Centres, 2013
5
Surveillance and Control of Animal Protozoan Diseases
(Indicate in the appropriate box the number by category)
a)
Articles published in peer-reviewed journals: ................. 55
Angeles JM, Kirinoki M, Goto Y, Asada M, Hakimi H, Leonardo L, et al. (2013). Localization and
expression profiling of a 31kDa antigenic repetitive protein Sjp_0110390 in Schistosoma japonicum
life stages. Mol Biochem Parasitol., 187, 98-102.
Cao S, Aboge GO, Terkawi MA, Zhou M, Luo Y, Yu L, Li Y, Goo Y, Kamyingkird K, Masatani T, Suzuki
H, Igarashi I, Nishikawa Y, Xuan X. (2013). Cloning, characterization and validation of inosine 5'monophosphate dehydrogenase of Babesia gibsoni as molecular drug target. Parasitol Int., 62, 8794.
Cao S, Luo Y, Aboge GO, Terkawi MA, Masatani T, Suzuki H, Igarashi I, Nishikawa Y, Xuan X (2013).
Identification and characterization of an interspersed repeat antigen of Babesia microti (BmIRA).
Exp Parasitol., 133., 346-352.
Cao S, Mousa AA, Aboge GO, Kamyingkird K, Zhou M, Moumouni PF, Terkawi MA, Masatani T,
Nishikawa Y, Suzuki H, Fukumoto S, Xuan X. (2013). Prime-boost vaccination with plasmid DNA
followed by recombinant vaccinia virus expressing BgGARP induced a partial protective immunity
to inhibit Babesia gibsoni proliferation in dogs. Acta Parasitol., 58, 619-623.
Cao S, Zhang S, Jia L, Xue S, Yu L, Kamyingkird K, Moumouni PF, Moussa AM, Zhou M, Zhang Y,
Terkawi MA, Masatani T, Nishikawa Y, Xuan X (2013). Prevalence of ovine Theileria spp in Northern
China. J Vet Med Sci., 75, 1227-1230.
Galay RL, Aung KM, Umemiya-Shirafuji R, Maeda H, Matsuo T, Kawaguchi H, Miyoshi N, Suzuki H,
Xuan X, Mochizuki M, Fujisaki K, Tanaka T (2013). Multiple ferritins are vital to successful blood
feeding and reproduction of the hard tick Haemaphysalis longicornis. J Exp Biol., 216, 1905-1915.
Goo YK, Ueno A, Terkawi MA, Oluga Aboge G, Junya Y, Igarashi M, Kim JY, Hong YC, Chung DI,
Nishikawa Y, Xuan X. (2013). Actin polymerization mediated by Babesia gibsoni aldolase is required
for parasite invasion. Exp Parasitol., 135, 42-49.
Hakimi H, Nguyen T-T, Suganuma K, Masuda-Suganuma H, Angeles JM, Inoue N, et al. (2013)
Development of monoclonal antibodies that target 1-Cys peroxiredoxin and differentiate
Plasmodium falciparum from P. vivax and P. knowlesi. Trop Med Health., 41, 55-59.
Ibrahim HM, Adjou Moumouni PF, Mohammed-Geba K, Sheir SK, Hashem IS, Cao S, Terkawi MA,
Kamyingkird K, Nishikawa Y, Suzuki H, Xuan X. (2013). Molecular and serological prevalence of
Babesia bigemina and Babesia bovis in cattle and water buffalos under small-scale dairy farming in
Beheira and Faiyum Provinces, Egypt. Vet Parasitol., 198, 187-192.
Ishiwa A, Kobayashi K, Takemae H, Sugi T, Gong H, Recuenco FC, Murakoshi F, Inomata A, Horimoto
T, Kato K. (2013). Effects of dextran sulfates on the acute infection and growth stages of
Toxoplasma gondii. Parasitol Res., 112, 4169-4176.
Iwanaga T, Sugi T, Kobayashi K, Takemae H, Gong H, Recuenco FC, Ishiwa A,Horimoto T, Akashi H,
Kato K. (2013). Function of Plasmodium falciparum cyclin-dependent kinases in erythrocytic
schizogony. Parasitol Int., 62, 423-430.
Jia L, Zhang S, Qian N, Xuan X, Yu L, Zhang X, Liu M (2013). Generation and immunity testing of a
recombinant adenovirus expressing NcSRS2-NcGRA7 fusion protein of bovine Neospora caninum.
Korean J Parasitol., 51, 247-253.
Kobayashi K, Takano R, Takemae H, Sugi T, Ishiwa A, Gong H, Recuenco FC, Iwanaga T, Horimoto T,
Akashi H, Kato K. (2013). Analyses of interactions between heparin and the apical surface proteins
6
Annual reports of OIE Reference Centres, 2013
Surveillance and Control of Animal Protozoan Diseases
of Plasmodium falciparum. Sci Rep., 3, 3178.
Komaki-Yasuda K, Okuwaki M, Nagata K, Kawazu S, Kano S. (2013). Identification of a novel and
unique transcription factor in the intraerythrocytic stage of Plasmodium falciparum. PLoS ONE, 8,
e74701.
Masatani T, Matsuo T, Tanaka T, Terkawi MA, Lee EG, Goo YK, Aboge GO, Yamagishi J, Hayashi K,
Kameyama K, Cao S, Nishikawa Y, Xuan X (2013). TgGRA23, a novel Toxoplasma gondii dense
granule protein associated with the parasitophorous vacuole membrane and intravacuolar
network. Parasitol Int., 62, 372-379.
Masatani T, Ooka H, Terkawi MA, Cao S, Luo Y, Asada M, Hayashi K, Nishikawa Y, Xuan X. (2013).
Identification, cloning and characterization of BmP41, a common antigenic protein of Babesia
microti. J Vet Med Sci., 75, 967-970.
Mirano-Bascos D, Tongol-Rivera P, Villacorte EA, Escueta AD, Kawazu S, Kano S. (2013).
Characterization of the population demographics and the MSP-1 block 2 allele gene frequencies of
P. falciparum infected individuals in Davao, Philippines. Trop Med Health., 41, 1-6.
Mochabo KM, Zhou M, Suganuma K, Kawazu S, Suzuki Y, Inoue N. (2013). Expression,
immunolocalization and serodiagnostic value of Tc38630 protein from Trypanosoma congolense.
Parasitol Res., 112, 3357-3363.
Mousa AA, Cao S, Aboge GO, Terkawi MA, Kirdasy AE, Salama A, Aboulaila M, Kamyingkird K,
Moumouni PF, Masatani T, Aziz SA, Moussa WM, Chahan B, Fukumoto S, Nishikawa Y, Ballal SS,
Xuan X. (2013). Molecular characterization and antigenic properties of a novel Babesia gibsoni
glutamic acid-rich protein (BgGARP). Exp Parasitol., 135, 414-420.
Munkhjargal T, Sivakumar T, Battsetseg B, Nyamjargal T, Aboulaila M, Purevtseren B, Bayarsaikhan
D, Byambaa B, Terkawi MA, Yokoyama N, Igarashi I. (2013). Prevalence and genetic diversity of
equine piroplasms in Tov province, Mongolia. Infect Genet Evol., 16C, 178-185.
Murakoshi F, Tozawa Y, Inomata A, Horimoto T, Wada Y, Kato K. (2013). Molecular
characterization of Cryptosporidium isolates from calves in Ishikari District, Hokkaido, Japan. J Vet
Med Sci., 75, 837-840.
Nagano D, Sivakumar T, de DE Macedo AC, Inpankaew T, Alhassan A, Igarashi I, Yokoyama N.
(2013). The Genetic Diversity of Merozoite Surface Antigen 1 (MSA-1) among Babesia bovis
detected from cattle populations in Thailand, Brazil and Ghana. J Vet Med Sci., 75, 1463-1470.
Namangala B, Oparaocha E, Kajino K, Hayashida K, Moonga L, Inoue N, et al. (2013). Short Report:
Preliminary investigation of trypanosomosis in exotic dog breeds from Zambia's Luangwa and
Zambezi Valleys using LAMP. Am J Trop Med Hyg., 89, 116-118.
Nelson B, Freisinger T, Ishii K, Okado K, Shinzawa N, Fukumoto S, Kanuka H. (2013). Activation of
Imd pathway in hemocyte confers infection resistance through humoral response in Drosophila.
Biochem Biophys Res Commun., 430, 1120-1125.
Nguyen QD, Nguyen T-T, Pham QP, Le NM, Nguyen GTT, Inoue N. (2013). Seroprevalence of
Trypanosoma evansi infection in water buffaloes from the mountainous region of North Vietnam
and effectiveness of trypanocidal drug treatment. J Vet Med Sci., 75, 1267-1269.
Nishikawa Y, Ogiso A, Kameyama K, Nishimura M, Xuan X, Ikehara Y. (2013). α2-3 sialic acid
glycoconjugate loss and its effect on infection with Toxoplasma parasites. Exp Parasitol., 135, 479485.
Nishimura M, Kohara J, Kuroda Y, Hiasa J, Tanaka S, Muroi Y, Kojima N, Furuoka H, Nishikawa Y.
(2013). Oligomannose-coated liposome-entrapped dense granule protein 7 induces protective
Annual reports of OIE Reference Centres, 2013
7
Surveillance and Control of Animal Protozoan Diseases
immune response to Neospora caninum in cattle. Vaccine, 31, 3528-35.
Nishimura M, Kohara J, Hiasa J, Muroi Y, Yokoyama N, Kida K, Xuan X, Furuoka H, Nishikawa Y.
(2013). Tissue distribution of Neospora caninum in experimentally infected cattle. Clin Vaccine
Immunol., 20, 309-312.
Okada T, Marmansari D, Li ZM, Adilbish A, Canko S, Ueno A, Shono H, Furuoka H, Igarashi M.
(2013). A novel dense granule protein, GRA22, is involved in regulating parasite egress in
Toxoplasma gondii. Mol Biochem Parasitol., 189, 5-13.
Quan JH, Cha GH, Zhou W, Chu JQ, Nishikawa Y, Lee YH. (2013). Involvement of PI 3 kinase/Aktdependent Bad phosphorylation in Toxoplasma gondii-mediated inhibiton of host cell apoptosis.
Exp Parasitol., 133, 462-471.
Saiki E, Nagao K, Aonuma H, Fukumoto S, Xuan X, Bannai M, Kanuka H (2013). Multivariable
analysis of host amino acids in plasma and liver during infection of malaria parasite Plasmodium
yoelii. Malar J., 12, 19-27.
Salama AA, Aboulaila M, Moussa AA, Nayel MA, El-Sify A, Terkawi MA, Hassan HY, Yokoyama N,
Igarashi I. (2013). Evaluation of in vitro and in vivo inhibitory effects of fusidic acid on Babesia and
Theileria parasites. Vet Parasitol., 191, 1-10.
Salama AA, Terkawi MA, Kawai S, Aboulaila M, Nayel M, Mousa A, Zaghawa A, Yokoyama N,
Igarashi I. (2013). Specific antibody to a conserved region of Babesia apical membrane antigen-1
inhibited the invasion of B. bovis into the erythrocyte. Exp Parasitol., 135, 623-628.
Simking P, Saengow S, Bangphoomi K, Sarataphan N, Wongnarkpet S, Inpankaew T, Jittapalapong S,
Munkhjargal T, Sivakumar T, Yokoyama N, Igarashi I. (2013). The molecular prevalence and MSA-2b
gene-based genetic diversity of Babesia bovis in dairy cattle in Thailand. Vet Parasitol., 197, 642648.
Sivakumar T, Lan DT, Long PT, Yoshinari T, Tattiyapong M, Guswanto A, Okubo K, Igarashi I, Inoue
N, Xuan X, Yokoyama N. (2013). PCR detection and genetic diversity of bovine hemoprotozoan
parasites in Vietnam. J Vet Med Sci., 75, 1455-1462.
Sivakumar T, Yoshinari T, Igarashi I, Kothalawala H, Abeyratne SA, Vimalakumar SC, Meewewa AS,
Kuleswarakumar K, Chandrasiri AD, Yokoyama N. (2013). Genetic diversity within Theileria
orientalis parasites detected in Sri Lankan cattle. Ticks Tick Borne Dis., 4, 235-241.
Sivakumar T, Okubo K, Igarashi I, de Silva WK, Kothalawala H, Silva SS, Vimalakumar SC, Meewewa
AS, Yokoyama N. (2013). Genetic diversity of merozoite surface antigens in Babesia bovis detected
from Sri Lankan cattle. Infect Genet Evol., 19, 134-140.
Suganuma K, Mochabo KM, Hakimi H, Yamasaki S, Yamagishi J, Asada M, et al. (2013). Adenosineuridine-rich element is one of the required cis-elements for epimastigote form stage-specific gene
expression of the congolense epimastigote specific protein. Mol Biochem Parasitol., 191, 36-43.
Sugi T, Kato K. (2013). Signaling cascade and stage differentiation of Toxoplasma gondii. Jpn J Vet
Parasitol., 12, 73-78.
Sugi T, Kobayashi K, Gong H, Takemae H, Ishiwa A, Iwanaga T, Horimoto T, Akashi H, Kato K. (2013).
Identification of mutations in TgMAPK1 of Toxoplasma gondii conferring the resistance to 1NMPP1. Int J Parasitol Drugs Drug Resist., 3, 93-101.
Tagawa, M., Ybanez, A.P., Matsumoto, K., Yokoyama, N., and Inokuma, H. (2013). Interference
between Theileria orientalis and hemotropic Mycoplasma spp.(hemoplasmas) in grazing cattle.
Vet. Parasitol., 195, 165-168.
8
Annual reports of OIE Reference Centres, 2013
Surveillance and Control of Animal Protozoan Diseases
Takemae H, Sugi T, Kobayashi K, Gong H, Ishiwa A, Recuenco FC, Murakoshi F, Iwanaga T, Inomata
A, Horimoto T, Akashi H, Kato K. (2013). Characterization of the interaction between Toxoplasma
gondii rhoptry neck protein 4 and host cellular β-tubulin. Sci Rep., 3, 3199.
Takashima Y, Takasu M, Yanagimoto I, Hattori N, Batanova T, Nishikawa Y, Kitoh K. (2013).
Prevalence and Dynamics of Antibodies against NcSAG1 and NcGRA7 Antigens of Neospora
caninum in Cattle during the Gestation Period. J Vet Med Sci., 75, 1413-1418.
Tanaka S, Nishimura M, Ihara F, Yamagishi J, Suzuki Y, Nishikawa Y. (2013). Transcriptome Analysis
of Mouse Brain Infected with Toxoplasma gondii. Infect Immun., 81, 3609-3619.
Terkawi MA, Kameyama K, Rasul NH, Xuan X, Nishikawa Y. (2013). Development of an
Immunochromatographic Assay Based on Dense Granule Protein 7 for Serological Detection of
Toxoplasma gondii Infection. Clin Vaccine Immunol., 20, 596-601.
Terkawi MA, Ratthanophart J, Salama A, Aboulaila M, Asada M, Ueno A, Alhasan H, Guswanto A,
Masatani T, Yokoyama N, Nishikawa Y, Xuan X, Igarashi I. (2013). Molecular Characterization of a
New Babesia bovis Thrombospondin-Related Anonymous Protein (BbTRAP2). PLoS One, 13, 8,
e83305.
Ueno A, Terkawi MA, Yokoyama M, Cao S, Aboge G, Aboulaila M, Nishikawa Y, Xuan X, Yokoyama
N, Igarashi I. (2013). Farsenyl pyrophosphate synthase is a potential molecular drug target of
risedronate in Babesia bovis. Parasitol Int., 28, 189-192.
Usui M, Masuda-Suganuma H, Fukumoto S, Angeles JM, Inoue N, Kawazu S. (2013). Expression
profiles of peroxiredoxins in liver stage of the rodent malaria parasite Plasmodium berghei.
Parasitol Int., 62, 337-340.
Turturice BA, Lamm MA, Tasch JJ, Zalewski A, Kooistra RL, Schroeter EH, et al. (2013). Expression of
cytosolic peroxiredoxins in Plasmodium berghei ookinetes is regulated by environmental factors in
the mosquito bloodmeal. PLoS Pathog., 9, e1003136.
Ybañez RH, Terkawi MA, Kameyama K, Xuan X, Nishikawa Y. (2013). Identification of a highly
antigenic region of subtilisin-like serine protease 1 for serodiagnosis of Neospora caninum
infection. Clin Vaccine Immunol., 20, 1617-1622.
Ybañez, A. P., Sivakumar, T., Battsetseg, B., Battur, B., Altangerel, K., Matsumoto, K., Yokoyama,
N., and Inokuma, H. (2013). Specific molecular detection and characterization of Anaplasma
marginale in Mongolian cattle. J. Vet. Med. Sci., 75: 399-406.
Ybañez, A.P., Tagawa, M., Matsumoto, K., Kishimoto, T., Yokoyama, N., and Inokuma H. (2013).
Specific Molecular Detection of Anaplasma sp. Closely Related to Anaplasma phagocytophilum in
Ixodid Ticks and Cattle in a Pastureland in Hokkaido, Japan. Vector Borne Zoonotic Dis., 13, 6-11.
Ybañez, A.P., Sivakumar, T., Ybañez, R.H., Vincoy, M.R., Tingson, J.A., Perez, Z.O., Gabotero, S.R.,
Buchorno, L.P., Inoue, N., Matsumoto, K., Inokuma, H., and Yokoyama, N. (2013). Molecular survey
of bovine vector-borne pathogens in Cebu, Philippines. Vet. Parasitol., 196, 13-20.
Yoshinari T, Sivakumar T, Asada M, Battsetseg B, Huang X, Lan DT, Inpankaew T, Ybañez AP,
Alhassan A, Thekisoe OM, Macedo AC, Inokuma H, Igarashi I, Yokoyama N. (2013). A PCR based
survey of Babesia ovata in cattle from various Asian, African, and South American Countries. J Vet
Med Sci., 75, 211-214.
Yu L, Terkawi MA, Cruz-Flores MJ, Claveria FG, Aboge GO, Yamagishi J, Goo YK, Cao S, Masatani T,
Nishikawa Y, Xuan X (2013). Epidemiological survey of Babesia bovis and Babesia bigemina
infections of cattle in Philippines. J Vet Med Sci., 75, 995-998.
Annual reports of OIE Reference Centres, 2013
9
Surveillance and Control of Animal Protozoan Diseases
b)
International conference presentations: ......................... 10
Hakimi H., and Kawazu SI. Plasmodium vivax and P. knowlesi: Cloning, expression and functional
analysis of peroxiredoxins. International Malaria Symposium, April 16-17, 2012, Kota Kinabalu,
Malaysia
Tatsuki Sugi, Hitoshi Takemae, Akiko Ishiwa, Taisuke Horimoto, Hiroomi Akashi, Kentaro Kato.
“Characterization of 1NM-PP1 resistant Toxoplasma gondii with mutation at TgMAPK1” 12th
International Congress on Toxoplasmosis, June, 2013, Oxford, U.K.
Kentaro Kato, Tatsuki Sugi, Taisuke Horimoto, Hiroomi Akashi. “Characterization of a Toxoplasma
gondii calcium calmodulin-dependent protein kinase homolog” 12th International Congress on
Toxoplasmosis, June, 2013, Oxford, U.K.
Angeles JM., Goto Y.,Kirinoki M., Leonardo L., Asada M., Rivera PT., Villacorte E., Inoue N., Chigusa
Y., and Kawazu S. Development of recombinant antigen-based serological diagnosis for the
detection of Schistosoma japonicum infection in water buffaloes. 24th International conference of
the world association for the advancement of veterinary parasitology, (WAAVP), August 25-29,
2013, Perth, Australia
Terkawi MA, Yokoyama N, ＆ Igarashi I. Characterisation of a new Babesia bovis thrombospondinrelated anonymous protein as a novel blood-stage vaccine candidate. WAAVP 2013: 24th
International Conference of the World Association for the Advancement of Veterinary Parasitology,
25-29 August, 2013, Perth, Australia
Angeles JMA., Goto Y., Kirinoki M., Leonardo L., Asada M., Hakimi H., Villacorte E., Tongol-Rivera
P., Inoue N., Chigusa Y., and Kawazu S. Development of serological techniques for the diagnosis of
zoonotic Schistosoma japonicum infection through the use of recombinant proteins. American
Society of Tropical Medicine and Hygiene 62th Annual Meeting, November 13-17, 2013,
Washington, DC, USA
Furuyama W., Enomoto M., Mossaad E., Kawai S., Mikoshiba K., and Kawazu S.Melatonin-cAMP
and IP3-Ca2+ signaling pathways control intraerythrocytic development of the malaria parasite
Plasmodium falciparum. American Society of Tropical Medicine and Hygiene 62th Annual Meeting,
November 13-17, 2013, Washington, DC, USA
Usui M., Masuda-Suganuma H., Fukumoto S., Angeles JMA., Inoue N., Kanzok MS., and Kawazu S.
Expression profiles of peroxiredoxins in liver stage of the rodent malaria parasite Plasmodium
berghei. American Society of Tropical Medicine and Hygiene 62th Annual Meeting, November 1317, 2013, Washington, DC, USA
Terkawi A, Maki Nishimura, Hidefumi Furuoka, Yoshifumi Nishikawa : Depletion of macrophages
during acute-phase infection of Plasmodium yoelii causes severe malaria associated with hepatic
and renal lesions. American Society of Tropical Medicine and Hygiene 62th Annual Meeting,
November 13-17, 2013, Washington, DC, USA
Mochabo K., Nguyen TT., Suganuma K., Mo Z., Kawazu S., Suzuki Y., and Inoue N. The potential use
of recombinant protein in enzyme-linked immunosorbent assay and immunochromatographic test
in the diagnosis of African trypa nosomosis. Joint International Tropical Medicine Meeting 2013
(JITMM 2013), December 11-13, 2013, Bangkok, Thailand
c)
National conference presentations: ................................ 55
Sivakumar T. et al. Molecular epidemiology and clinical significance of Theileria orientalis and
Babesia ovata in cattle. The 155th Annual Meeting of the Japanese Society of Veterinary Science,
28-30 March, 2013, Tokyo, Japan
Kamyingkird K. et al. Seroprevalence of equine piroplasmosis in horses, mules and donkey in
Northern Thailand. The 155th of Annual Meeting of The Japanese Society of Veterinary Science, 2830 March, Tokyo, Japan.
Angeles JM., Kirinoki M., Goto Y., Asada M., Leonardo L., Rivera PT., Villacorte E., Inoue N., Chigusa
Y., and Kawazu S. Development of recombinant antigen-based serological diagnosis for the
10
Annual reports of OIE Reference Centres, 2013
Surveillance and Control of Animal Protozoan Diseases
detection of Schistosoma japonicum infection in water buffaloes. The 155th Annual Meeting of the
Japanese Society of Veterinary Science, 28-30 March, 2013, Tokyo, Japan
Hakimi H., Nguyen TT., Suganuma K., Masuda-Suganuma H., Inoue N., and Kawazu SI.
Development of monoclonal antibodies against Plasmodium falciparum 1-Cys peroxiredoxin and its
possible application for malaria diagnosis. The 155th Annual Meeting of the Japanese Society of
Veterinary Science, 28-30 March, 2013, Tokyo, Japan
Paul Franck Adjou Moumouni et al. Molecular characterizations of Babesia bovis and B. bigemina
isolated from cattle and water buffalo in Egypt and Kenya. The 155th Annual Meeting of the
Japanese Society of Veterinary Science, 28-30 March, 2013, Tokyo, Japan
Cao S. et al. Cloning, characterization and validation of inosine 5'-monophosphate dehydrogenase
of Babesia gibsoni as molecular drug target. The 155th Annual Meeting of the Japanese Society of
Veterinary Science, 28-30 March, 2013, Tokyo, Japan
Recuenco F. et al. In vitro growth inhibition activities of modified carrageenans and gellan gum
against Plasmodium falciparum 3D7. The 155th Annual Meeting of the Japanese Society of
Veterinary Science, 28-30 March, 2013, Tokyo, Japan
Terkawi A. et al. Macrophages are crucial for determining the outcome of blood-stage non-lethal
Plasmodium yoelii infection in mice. The 82nd Annual Meeting of the Japanese Society of
Parasitology, 29-31 March, 2013, Tokyo, Japan
Hatta T. et al. mRNA-seq analysis of the midgut of Babesia parasitized tick fed on newly developed
artificial blood-feeding systsem. The 82nd Annual Meeting of the Japanese Society of Parasitology,
29-31 March, 2013, Tokyo, Japan
Igarashi I. et al. Discovery of a new Babesia bovis thrombospondin-related anonymous protein as a
novel blood-stage vaccine candidate. The 82nd Annual Meeting of the Japanese Society of
Parasitology, 29-31 March, 2013, Tokyo, Japan
Angeles JM., Kirinoki M., Goto Y., Asada M., Hakimi H., Leonardo L., Rivera PT., Villacorte E., Inoue
N., Chigusa Y., and Kawazu S. Localization and expression profiling of a 31 kDa antigenic repetitive
protein Sjp_0110390 in Schistosoma japonicum life stages. The 82nd Annual Meeting of the
Japanese Society of Parasitology, 29-31 March, 2013, Tokyo, Japan.
Hakimi H., Nguyen TT., Suganuma K., Masuda-Suganuma H., Inoue N., and Kawazu SI.
Development of monoclonal antibodies targeting 1-Cys peroxiredoxin which differentiate
Plasmodium falciparum from P. vivax and P. knowlesi. The 82nd Annual Meeting of the Japanese
Society of Parasitology, 29-31 March, 2013, Tokyo, Japan
Cao S. et al. Cloning, characterization and validation of inosine 5'-monophosphate dehydrogenase
of Babesia gibsoni as molecular drug target. The 82nd Annual Meeting of the Japanese Society of
Parasitology, 29-31 March, 2013, Tokyo, Japan
Recuenco F. et al. In vitro growth inhibition activities of modified carrageenans and gellan gum
against Plasmodium falciparum 3D7. The 82nd Annual Meeting of the Japanese Society of
Parasitology, 29-31 March, 2013, Tokyo, Japan
Terkawi A. et al. Macrophages are crucial for determining the outcome of blood-stage non-lethal
Plasmodium yoelii infection in mice. The 82nd Annual Meeting of the Japanese Society of
Parasitology, 29-31 March, 2013, Tokyo, Japan
Salama A. et al. Inhibitory effect of allicin on the growth of Babbesia and Theileria equi parasites.
The 156th Annual Meeting of the Japanese Society of Veterinary Science, 20-22 September, 2013,
Gifu, Japan
Recuenco F. et al. Assessing the microbial polysaccharide gellan gum and its sulfated derivative on
their inhibition of growth of Plasmodium yoelii 17XL in BALB/c mice. The 156th Annual Meeting of
the Japanese Society of Veterinary Science, 20-22 September, 2013, Gifu, Japan
Angeles JM., Goto Y., Leonardo L., Kirinoki M., Hakimi H., Rivera PT., Villacorte E., Inoue N., Chigusa
Y., and Kawazu S. Detection of canine schistosomiasis japonica using recombinant thioredoxin
peroxidase-1 and tandem repeat proteins. The 54th of Japanese Society of Tropical Medicine and
Hygiene. 5 October 2013, Nagasaki, Japan
Presentations of 19 to 54 were written in Japanese and thereforethey were not described here.
d)
Other: ...............................................................................
Annual reports of OIE Reference Centres, 2013
11
Surveillance and Control of Animal Protozoan Diseases
(Provide website address or link to appropriate information)
_______________
12
Annual reports of OIE Reference Centres, 2013