Carroll et al: Paternity assignment & demographic closure in right whales: Supplementary Material
Supplementary Table 1: Three microsatellite loci used to augment the microsatellite genotype of southern right whales. Primer
sequences and repeat units identified from the reference indicated. T A is the annealing temperature; mM Mg is the concentration of
magnesium used in the reactions. Each 10 μL PCR reaction contained 1xPCR buffer, MgCl2 at concentration specified below, 0.4 μM
each primer, 0.2 mM dNTPs, 0.25 units thermostable Platinum Taq DNA polymerase (Invitrogen) and 10-20 ng DNA template. The PCR
reactions have cycling conditions of (i) an initial denaturing step at 94° C for 3 min; (ii) 30 cycles at 94° C for 30 sec, T A for 30 sec and
72° C for 30 sec; and (iii) a final extension step at 72° C for 10 min.
Locus
GT23
Primers
F: GTTCCCAGGCTCTGCACTCTG
Label
TA (ºC)
mM Mg
Repeat Unit
Reference
VIC
58
2.0
(GT)n
Bérubé, Jørgensen et al.(2000)
NED
53
2.5
(TG)n
Waldick, Brown et al. (1999)
FAM
60
2.0
(GATA)n
Frasier, Rastogi et al. (2006)
R:CATTTCCTACCCACCTGTCAT
RW34
F: CACTCAAGCCCCATAACG
R: TAGTAGAGCCGTGATAAAGTGC
TR3G10
F: GCTCCGCAACAAGAGAGG
R: GCACATGACGCTCAGTGC
Carroll et al: Paternity assignment & demographic closure in right whales: Supplementary Material
Supplementary Table 2: DNA profiles of parent-offspring tryads: mtDNA control region haplotype (500 bp; mtDNA), genetically identified
sex and microsatellite genotype. Dashed lines indicate the sample was not successfully genotyped at that locus. MM indicates a locus
at which the assigned father offspring pair mismatched.
Sample
Eau03NZ03
Eau03NZ02
Eau97AI071
Eau05NZ03
Eau05NZ02
Eau06AI135
Eau06AI037
Eau06AI038
Eau06AI059
Eau07AI087
Eau07AI088
Eau95AI033
Eau07AI179
Eau07AI180
Eau98AI069
Eau07AI190
Eau07AI191
Eau07AI158
Eau07AI196
Eau07AI026
Eau98AI088
Eau08AI081
Eau08AI082
Eau08AI061
Eau08AI181
Eau08AI151
Eau07AI159
Eau09AI159
Eau09AI238
Eau06AI111
Status
calf
mother
father
calf
mother
father
calf
mother
father
calf
mother
father
calf
mother
father
calf
mother
father
calf
mother
father
calf
mother
father
calf
mother
father
calf
mother
father
Sex
M
F
M
F
F
M
M
F
M
M
F
M
F
F
M
F
F
M
F
F
M
F
F
M
F
F
M
M
F
M
mtDNA
B'
B'
A
A
A
A
D
D
A
B+
A
A
A
A
B'
B'
B+
D
D
B+
A
A
C
A
A
B+
B+
B+
D
MM
GATA28
TR3G2
GATA28
RW31
EV14
EV1
124/126
124/126
126/140
122/122
122/136
122/148
122/140
122/140
122/126
126/126
126/126
124/126
132/144
138/144
126/132
122/140
122/126
124/140
122/124
122/122
124/140
126/134
126/132
130/134
126/128
128/136
126/126
130/140
122/140
-/-
EV14
122/135
133/135
122/129
133/133
120/133
133/135
129/141
129/141
133/141
133/135
133/133
133/135
131/133
133/139
131/137
133/135
135/141
120/133
135/141
135/141
122/135
137/141
133/137
137/141
133/137
133/137
133/133
122/122
120/122
133/133
EV37
189/199
199/203
189/189
203/205
187/203
197/205
203/203
197/203
189/203
191/199
191/201
199/207
201/203
201/203
193/203
189/199
199/203
189/189
189/203
201/203
189/201
207/207
207/207
189/207
195/207
197/207
195/203
197/205
201/205
197/203
GATA28
166/180
166/166
166/180
166/174
166/174
168/178
166/178
166/178
166/178
166/166
166/178
166/174
166/174
162/174
166/166
170/178
170/174
166/180
170/174
170/178
174/178
166/166
162/166
166/178
162/166
166/178
162/166
162/178
178/178
162/178
GATA98
116/116
108/116
104/116
112/124
104/124
112/116
116/120
112/120
116/116
112/116
112/112
116/116
108/112
108/112
104/112
116/120
116/116
112/120
104/112
112/112
104/116
112/116
104/112
112/116
108/116
108/116
116/116
108/112
112/120
108/112
GT23
114/116
-/114/116
116/116
116/118
114/116
108/108
108/110
108/110
110/112
112/114
110/114
106/114
106/112
114/118
110/112
110/110
112/118
108/116
116/118
108/112
112/112
112/116
112/114
114/116
112/114
116/118
112/114
112/114
114/116
RW18
199/217
193/217
195/199
193/193
189/193
187/193
193/213
189/193
193/213
193/231
195/231
193/193
195/195
195/233
193/195
-/187/189
193/193
189/195
187/195
189/213
187/193
187/197
187/193
193/197
197/199
193/195
193/193
193/193
193/199
RW31
125/125
123/125
125/127
119/125
123/125
125/125
121/123
123/123
121/131
123/123
123/123
123/125
123/125
125/125
123/125
123/123
123/125
121/123
125/125
121/125
125/125
121/121
117/121
123/125
121/123
121/125
123/125
121/123
123/123
121/125
RW410
195/203
195/205
203/211
191/205
191/203
205/207
211/211
191/211
191/211
203/211
203/207
205/211
199/205
195/205
199/211
205/211
199/211
205/205
197/211
209/211
197/197
195/211
195/211
191/195
209/211
210/211
195/209
205/209
201/205
209/209
RW48
118/120
118/126
118/120
120/126
118/126
120/120
120/124
120/126
124/124
108/118
108/122
108/118
108/118
118/120
108/126
124/126
108/126
108/124
118/120
120/122
118/118
124/124
118/124
118/124
108/122
120/122
108/118
118/120
108/120
118/122
Carroll et al: Paternity assignment & demographic closure in right whales: Supplementary Material
Supplementary Table 2 continued
Sample
Eau03NZ03
Eau03NZ02
Eau97AI071
Eau05NZ03
Eau05NZ02
Eau06AI135
Eau06AI037
Eau06AI038
Eau06AI059
Eau07AI087
Eau07AI088
Eau95AI033
Eau07AI179
Eau07AI180
Eau98AI069
Eau07AI190
Eau07AI191
Eau07AI158
Eau07AI196
Eau07AI026
Eau98AI088
Eau08AI081
Eau08AI082
Eau08AI061
Eau08AI181
Eau08AI151
Eau07AI159
Eau09AI159
Eau09AI238
Eau06AI111
Status
calf
mother
father
calf
mother
father
calf
mother
father
calf
mother
father
calf
mother
father
calf
mother
father
calf
mother
father
calf
mother
father
calf
mother
father
calf
mother
father
TR3F4
301/305
301/317
305/309
317/325
301/317
305/325
305/305
305/313
305/313
301/333
329/333
301/320
309/313
313/333
305/309
305/309
305/333
305/309
313/329
305/329
301/313
305/313
305/313
313/329
305/317
317/337
305/305
308/345
305/308
329/345
TR3G1
222/222
222/238
222/222
218/238
222/238
218/222
222/238
-/222/238
222/238
206/238
222/222
206/222
222/238
206/206
206/238
206/222
222/238
238/238
-/238/238
206/218
206/238
214/218
202/238
238/238
202/206
206/214
202/214
-/-
TR3G2
168/184
172/184
168/184
172/184
172/184
172/176
168/180
168/172
168/180
176/188
172/188
172/176
176/188
176/184
176/188
172/184
172/184
168/180
172/176
172/176
172/176
180/184
176/180
180/184
176/184
176/184
176/184
176/176
176/184
176/180
GT211
85/96
85/89
96/98
85/97
-/97/101
97/99
97/103
99/99
85/103
97/103
85/85
85/97
85/97
85/97
85/99
85/99
99/103
101/103
97/103
85/101
95/103
95/97
99/103
97/103
95/103
95/97
85/101
101/103
-/-
RW51
91/109
103/109
91/99
83/109
83/109
105/109
83/95
-/95/95
91/91
91/99
91/91
91/91
-/91/97
99/99
-/99/103
107/107
-/107/107
95/103
-/95/95
103/105
101/105
91/103
99/99
99/99
-/-
TR3G10
214/214
214/214
214/214
214/222
214/214
206/222
214/222
206/222
214/214
214/214
214/214
214/214
214/214
214/222
214/214
214/214
214/222
214/222
214/222
214/214
214/222
214/222
214/222
214/222
214/222
214/222
206/214
-/214/214
-/-