Supplementary information
Manure removal system influences the abundance and composition of
airborne biotic contaminants in swine confinement buildings
Priyanka Kumari and Hong-Lim Choi*
Department of Agricultural Biotechnology and Research Institute for Agriculture and Life
Science, Seoul National University, Seoul, 151-921, South Korea.
* Correspondence: Department of Agricultural Biotechnology and Research Institute for
Agriculture and Life Science, Seoul National University, Seoul, 151-921, South Korea. Tel.:
+82 02 880 4808; fax: +82 02 874 4808; e-mail: ulsoo8@snu.ac.kr
Table S1 Primers used in qPCR to quantify the abundance of 16S rRNA and tetracycline
resistance genes
Primer
Target gene
Sequence (5’-3’)
names
mechanism
338F
16S rRNA
519R
gene
GWA TTA CCG CGG CKG
TetB-FW
tetB
TAC GTG AAT TTA TTG CTT CGG
TetB-RV
TetH-FW
tetH
tetZ
tetO
tetQ
TetQ-RV
TetW-FW
TetW-RV
Efflux pumps1
CAG TGA AAA TTC ACT GGC AAC
CCT TCT CGA CCA GGT CGG
ACC CAC AGC GTG TCC GTC
TetO-RV
TetQ-FW
Not applicable
ATC CAA AGT GTG GTT GAG AAT
TetZ-RV
TetO-FW
ACT CCT ACG GGA GGC AGC
ATA CAG CAT CCA AAG CGC AC
TetH-RV
TetZ-FW
Resistance
ACG GAR AGT TTA TTG TAT ACC
Ribosomal
TGG CGT ATC TAT AAT GTT GAC
protection
AGA ATC TGC TGT TTG CCA GTG
proteins2
CGG AGT GTC AAT GAT ATT GCA
tetW
GAG AGC CTG CTA TAT GCC AGC
GGG CGT ATC CAC AAT GTT AAC
References
1
Aminov RI, Chee-Sanford JC, Garrigues N, Teferedegne B, Krapac IJ, White BA, Mackie
RI. Development, validation, and application of PCR primers for detection of tetracycline
efflux genes of gram-negative bacteria. Appl Environ Microbiol 2002;68:1786-1793.
2
Aminov RI, Garrigues-Jeanjean N, Mackie RI. Molecular ecology of tetracycline resistance:
development and validation of primers for detection of tetracycline resistance genes encoding
ribosomal protection proteins. Appl Environ Microbiol 2001;67:22-32.