Supplementary File 1. Oligonucleotides used in this study
Sequence (5’→3’)
Primer function/target
47125
TTCATTATGGTGAAAGTTGGAACC
Screening of BRD948 gyrA::pWT-gyrA derivatives and
BRD948 aroC::pWT-aroC derivatives *
Cat-12
GyrA-07
GyrA-10
CCATAATCAGTCGACGGTATCGATAAGCTT
GGGTCGACTGATTATGGTTTATGCCTCC
GCGCATGCCACGACCGGTACGGTAG
Amplification of pJCB12 fragment
Construction of all gyrA mutations *
Construction of all gyrA mutations *
GyrA-11
TCGCGTCAGCTTCAACTTCC
Screening of BRD948 gyrA::pWT-gyrA derivatives *
GyrA-8
GyrA-9
GyrA-32
GyrA-33
GyrA-41
GyrA-42
GyrA-34
GyrA-35
GyrA-47
GyrA-48
GyrA-49
GyrA-50
GyrA-51
GyrA-52
GyrA-53
GyrA-54
ParC-18
ParC-21
ParC-23
ParC-27
ParC-28
R6K-01
R6K-03P
aroC-1
aroC-2
aroC-3
aroC-4
aroC-5
pyro-gyrA83-F
pyro-gyrA83-R
pyro-gyrA83-S
pyro-gyrA87-F
pyro-gyrA87-R
pyro-gyrA87-S
pyro-parC80-F
pyro-parC80-R
pyro-parC80-S
pyro-aroC-F
pyro-aroC-R
pyro-aroC-S
TCATACACTGCGTAATCGCCGTGGGGATG
CCCACGGCGATTACGCAGTGTATGACACC
TCATACACTGCGAAATCGCCGTGGGGATG
CCCACGGCGATTTCGCAGTGTATGACACC
AACGATGGTGTTATACACTGCGGAATC
TTCCGCAGTGTATAACACCATCGTTCG
AACGATGGTGTTATACACTGCGAAATC
TTTCGCAGTGTATAACACCATCGTTCG
AACGATGGTGCCATACACTGCGAAATC
TTTCGCAGTGTATGGCACCATCGTTCG
CGATGGTGGCATACACTGCGAAATC
CGCAGTGTATGCCACCATCGTTCG
CGATGGTGCCATACACTGCGAAATC
CGCAGTGTATGGCACCATCGTTCG
AACGATGGTGGCATACACTGCGAAATC
TTTCGCAGTGTATGCCACCATCGTTCG
GCGGTCGACGTTGGCGTGGTGAATAG
CGCGCATGCGGCAACGCGGTGATCA
GCCAGCAGTCAAACTTTTGG
ATAGCAGGCGATGTCGCCATG
CATGGCGACATCGCCTGCTAT
GTGACACAGGAACACTTAACGGC
P-CCATGTCAGCCGTTAAGTGTTC
ATGTCGACCCACTTCGCTGGCCTTATTAG
GTGCATGCGGCAAAGCTACCGATAGACT
GAGTTGTCCAATTGAGTTTCCTGCCATC
GGCAGGAAACTCAATTGGACAACTCTTTCG
CGAAATAACGGCGCTGATCGGTG
TGGGCAATGACTGGAACAAAG
biotin-TACCGTCATAGTTATCCACG
CGGTAAATACCATCCCCA
TGGGCAATGACTGGAACAAAG
biotin-TACCGTCATAGTTATCCACG
CGATTCCGCAGTGTA
biotin-ACCGTTGGCGACGTACTGG
AATTCCCCTGGCCATCGAC
CATGGCTTCATAGCAG
Biotin-GGCCCGTGAACATTTCAA
GTTACGCGAAAGAGTTGTCCA
CGAAAGAGTTGTCCAAT
Construction of gyrA S83Y *
Construction of gyrA S83Y *
Construction of gyrA S83F *
Construction of gyrA S83F *
Construction of gyrA D87N *
Construction of gyrA D87N *
Construction of gyrA S83F and D87N *
Construction of gyrA S83F and D87N *
Construction of gyrA S83F and D87G
Construction of gyrA S83F and D87G
Construction of gyrA D87A
Construction of gyrA D87A
Construction of gyrA D87G
Construction of gyrA D87G
Construction of gyrA S83F and D87A
Construction of gyrA S83F and D87A
Construction of all parC mutations
Construction of all parC mutations
Screening of BRD948 parC::pWT-parC derivatives
Construction of parC S80I mutation
Construction of parC S80I mutation
Screening of BRD948-parC::pWT-parC derivatives
Amplification pJCB12 fragment
Construction of all aroC mutation
Construction of all aroC mutation
Construction of aroC mutation ACA→ TCA
Construction of aroC mutation ACA → TCA
Screening for BRD 948 aroC::pWT-aroC derivatives
Pre-pyrosequencing amplification of gyrA codon 83
Pre-pyrosequencing amplification of gyrA codon 83
Pyrosequencing of gyrA codon 83
Pre-pyrosequencing amplification of gyrA codon 87
Pre-pyrosequencing amplification of gyrA codon 87
Pyrosequencing of gyrA codon 87
Pre-pyrosequencing amplification of parC codon 80
Pre-pyrosequencing amplification of parC codon 80
Pyrosequencing of parC codon 80
Pre-pyrosequencing amplification of aroC
Pre-pyrosequencing amplification of aroC
Pyrosequencing of aroC
Primer name
* As described in Turner et al. Nucleotides in which a substitution was induced are in bold and SalI and SphI restriction
endonuclease sites used in pJCB12 ligation are underlined