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Supplementary Table S2. Classification ”carbon utilization” indicates that 2% glucose was substituted with the indicated carbon source, classification “nitrogen utilization” indicates that 0.5% ammonium sulfate was substituted with the indicated nitrogen sources at nitrogen limiting concentrations. In all nitrogen utilization experiments, two consecutive pre-cultures were performed to deplete internal nitrogen storages: the first with nitrogen limiting amounts of ammonium, the second with nitrogen limiting amounts of the indicated nitrogen source. # = pre-cultures were performed in medium similar to the experimental medium to deplete internal storages of the molecule Environment No stress Lactose 2% Mannitol 2% Melezitose 2% Methanol 2% Starch 1% Trehalose 2% Xylitol 2% Citric acid 46mg/mL Citric acid 55mg/mL Citric acid 62.5mg/mL DHA 100mM DHA 200mM Formaldehyde 1mM Heat 41°C Heat 42°C Heat 43°C Malic acid 52mg/ml Malic acid 62.5mg/ml Methanol 10% Methanol 2% Methanol 5% Methanol 7.5% Methylglyoxal 2mM Methylglyoxal 4mM Oxalic acid 12.5mg/ml Oxalic acid 8mg/ml Selenomethionine 0.03mM Selenomethionine 0.06mM Synthetic wine must Tartaric acid 45mg/mL Tartaric acid 60mg/mL YPD Adenine Alanine Arginine Asparagine Class Carbon utilization Carbon utilization Carbon utilization Carbon utilization Carbon utilization Carbon utilization Carbon utilization Environment & Metabolites Environment & Metabolites Environment & Metabolites Environment & Metabolites Environment & Metabolites Environment & Metabolites Environment & Metabolites Environment & Metabolites Environment & Metabolites Environment & Metabolites Environment & Metabolites Environment & Metabolites Environment & Metabolites Environment & Metabolites Environment & Metabolites Environment & Metabolites Environment & Metabolites Environment & Metabolites Environment & Metabolites Environment & Metabolites Environment & Metabolites Environment & Metabolites Environment & Metabolites Environment & Metabolites Environment & Metabolites Nitrogen utilization Nitrogen utilization Nitrogen utilization Nitrogen utilization Aspartic Citrulline Cytidine Cytosine GABA Glutathione Glycine Glycine Guanidine Histidine Isoleucine Leucine Low Methionine Ornithine Phenylalanine Proline Serine Threonine Tryptophane Tyrosine Urea Valine Adenine (-) Aminoacids (-)# Biotin (-) Biotin (-)# Boron (-)# Ca (-) Ca (-)# Cu (-) Fe (-)# Inositol (-)# Inositol (-) Leucine (-)# Lysine (-) Methionine (-) Mg (-)# Mg (-) Mn (-)# Mo (-)# Na (-)# Nicotinamide (-) Nicotinamide (-)# PABA (-) PABA (-)# Nitrogen utilization Nitrogen utilization Nitrogen utilization Nitrogen utilization Nitrogen utilization Nitrogen utilization Nitrogen utilization Nitrogen utilization Nitrogen utilization Nitrogen utilization Nitrogen utilization Nitrogen utilization Nitrogen utilization Nitrogen utilization Nitrogen utilization Nitrogen utilization Nitrogen utilization Nitrogen utilization Nitrogen utilization Nitrogen utilization Nitrogen utilization Nitrogen utilization Nitrogen utilization Nutrient requirements Nutrient requirements Nutrient requirements Nutrient requirements Nutrient requirements Nutrient requirements Nutrient requirements Nutrient requirements Nutrient requirements Nutrient requirements Nutrient requirements Nutrient requirements Nutrient requirements Nutrient requirements Nutrient requirements Nutrient requirements Nutrient requirements Nutrient requirements Nutrient requirements Nutrient requirements Nutrient requirements Nutrient requirements Nutrient requirements Pantothenate (-) Pantothenate (-)# Phosphate (-)# Phosphate (-) Pyridoxin (-)# Riboflavin (-)# Thiamine (-)# Thiamine (-) Tryptophan (-)# Uracil (-) Zn (-)# 1,10 Phenanthroline 2µM 1,10 phenantroline 4µM 6-azauracil 200µg/mL 6-azauracil 300µg/mL 6-azauracil 500µg/mL Canavanine 100µg/mL DMSO 0.25% DMSO 2% DMSO 4% DMSO 8% DTT 1.6mM DTT 1.8mM Geneticin 2mM Geneticin 3mM Geneticin 4mM Hog1 inhibitor TA47:2 2µM Hog1p inhibitor TA47:2 8uM Hygromycin 0.6mg/mL Hygromycin B 1.25mg/ml Mercaptoethanol 15mM Mercaptoethanol 30mM Noursethricine 10µg/mL Noursethricine 100µg/mL Noursethricine 5µg/ml Staurosporine 10ug/mL Staurosporine 1ug/mL Thiabendazole 25µg/ml Thiabendazole 50µg/ml Nutrient requirements Nutrient requirements Nutrient requirements Nutrient requirements Nutrient requirements Nutrient requirements Nutrient requirements Nutrient requirements Nutrient requirements Nutrient requirements Nutrient requirements Toxins Toxins Toxins Toxins Toxins Toxins Toxins Toxins Toxins Toxins Toxins Toxins Toxins Toxins Toxins Toxins Toxins Toxins Toxins Toxins Toxins Toxins Toxins Toxins Toxins Toxins Toxins Toxins