Supporting information for MS entitled
Physiological response of the green microalgae Dunaliella tertiolecta against imidazolium ionic liquids
[bmim][BF4] and/or [omim][BF4]: the role of salinity on the observed effects.
Authors
Vasiliki Tsarpali1, Kassiani Harbi1 and Stefanos Dailianis1*
1
*
Section of Animal Biology, Department of Biology, Faculty of Sciences, University of Patras, 26500,
Patras, Greece.
Corresponding author:
Tel.: +32610-969213
Fax: +32610-969213
E-mail: sdailianis@upatras.gr
Section of Animal Biology
Department of Biology
Faculty of Sciences, University of Patras
26 500 PATRAS
GREECE
Table of contents
SM Table 2.1 Number of cells (x104 cells mL-1) of algae treated with [bmim][BF4],
[omim][BF4] and/or [bmim][BF4]-[omim][BF4] in culture media with different
salinities (A: 30‰ and B: 35‰). The results are mean ± SDs from 4 independent
experiments in each case. Values that share the same letter in each raw differ
significantly from each other (Mann–Whitney u-test, p<0.05). ................................ 3
SM Figure 2.1 Inhibition rates (%) in algal cells treated with (A) [bmim][BF4], (B)
[omim][BF4] and (C) [bmim][BF4]-[omim][BF4] (dilution ratio 1:1) for 24, 48, 72
and 96h, in culture media with different salinities (30 and 35‰). The results are
mean ± SD from 4 independent experiments in each case. ....................................... 4
2
SM Table 2.1 Number of cells (x104 cells mL-1) of algae treated with [bmim][BF4], [omim][BF4] and/or [bmim][BF4]-[omim][BF4] in culture media with different salinities
(A: 30‰ and B: 35‰). The results are mean ± SDs from 4 independent experiments in each case. Values that share the same letter in each raw differ significantly from each
other (Mann–Whitney u-test, p<0.05).
A
Incubation (h)
0
1
24h
48h
3.34±0.12abcd
8.98±1.31ab
2.87±0.12a
8.00±0.25
72h
96h
15.94±2.24ab
28.35±1.90abcd
13.50±1.47
24.16±1.65a
24h
48h
72h
96h
3.33±0.10abcd
8.98±0.93abcd
15.94±2.24abcd
28.35±1.90abcd
2.43±0.12a
4.12±0.12a
10.50±1.13a
20.00±0.41a
24h
48h
72h
96h
3.34±0.10abc
8.98±1.31 abcd
15.94±2.75 abcd
28.35±2.33 abcd
3.29±0.04
4.00±0.00a
3.33±0.14a
5.00±0.25a
B
Incubation (h)
24h
48h
72h
96h
0
4.39±0.57abcd
8.56±0.60abcd
10.98±1.72abcd
24.55±8.00abc
1
2.92±0.42a
3.00±0.00a
5.38±0.13a
13.63±0.13a
24h
48h
72h
96h
4.39±0.46abcd
8.56±0.60abcd
10.98±1.40abcd
24.55±6.53abcd
3.44±0.42a
5.77±0.78a
7.25±0.29a
10.08±2.83a
24h
48h
72h
4.39±0.46ab
8.56±0.60abcd
10.98±1.72abcd
5.00±0.00a
6.78±0.48a
8.42±0.72a
96h
24.55±6.53ab
17.56±0.42
[bmim][BF4] (mg L-1)
10
2.83±0.16b
8.16±0.16
13.33±1.70
20.50±1.22b
[omim][BF4] (mg L-1)
2.00±0.00b
3.33±0.20b
6.58±0.62b
12.05±1.00b
[bmim][BF4]-[omim][BF4] (mg L-1)
2.62±0.14a
3.61±0.78b
2.12±0.12b
4.50±0.00b
[bmim][BF4] (mg L-1)
10
2.25±0.25b
3.00±0.00b
6.04±0.71b
12.50±0.50b
[omim][BF4] (mg L-1)
2.61±0.08b
4.78±0.48b
5.33±0.39b
7.50±1.29b
[bmim][BF4]-[omim][BF4] (mg L-1)
4.50±0.00
5,75±0.66b
7.44±0.39b
16.56±0.63
3
100
200
2.72±0.25c
6.25±0.25a
2.44±0.09d
6.00±1.73b
11.56±1.00a
15.66±0.98c
9.33±0.47b
14.94±0.39d
1.66±0.11c
2.65±0.13c
4.00±0.20c
5.20±0.66c
0.50±0.00d
0.37±0.12d
1.00±0.00d
1.00±0.00d
1.91±0.16b
2.00±0.00c
1.54±0.21c
2.50±0.00c
1.25±0.00c
1.00±0.00d
1.00±0.00d
1.00±0.25d
100
2.08±0.14c
3.75±0.25c
6.13±0.13c
14.50±0.50c
200
2.04±0.29d
3.79±0.46d
6.25±0.25d
16.67±1.67
2.11±0.26c
2.00±0.00c
1.40±0.19c
1.17±0.19c
1.00±0.00d
1.00±0.00d
1.00±0.00d
1.00±0.00d
3.83±0.19
5.03±0.98c
6.25±0.75c
3.33±0.24b
2.58±0.10d
2.17±0.44d
14.67±1.25a
2.42±0.10b
A
120
120
30‰
100
Inhibition rate (%)
Inhibition rate (%)
100
80
1
60
10
100
40
200
20
120
48
72
incubation period (h)
100
200
24
120
30‰
48
72
incubation period (h)
96
35‰
100
Inhibition rate (%)
Inhibition rate (%)
10
40
96
100
80
1
60
10
100
40
200
80
1
60
10
100
40
200
20
20
0
0
24
120
48
72
incubation period (h)
24
96
120
30‰
48
72
incubation period (h)
96
35‰
100
Inhibition rate (%)
100
Inhibition rate (%)
1
60
0
24
C
80
20
0
B
35‰
80
1
60
10
100
40
200
80
1
60
10
100
40
200
20
20
0
0
24
48
72
incubation period (h)
24
96
48
72
incubation period (h)
96
SM Figure 2.1 Inhibition rates (%) in algal cells treated with (A) [bmim][BF4], (B) [omim][BF4] and (C)
[bmim][BF4]-[omim][BF4] (dilution ratio 1:1) for 24, 48, 72 and 96h, in culture media with different salinities (30 and
35‰). The results are mean ± SD from 4 independent experiments in each case.
4