Table S2. Genes significantly up-regulated in the pstCA mutant strain compared to wildtype C. rodentium
Functional class and
gene name/ORFa,b
Cell envelope
yibDb
ybhT
bcsC
slyB
ygaM
pagP
lppA
yecN
mipA
Function
Putative glycosyl transferase
Small membrane protein involved in the cell
envelope stress response
Cellulose synthase operon protein C
Outer membrane lipoprotein
Predicted protein
Palmitoyl transferase for lipid A, putative
antimicrobial peptide resistance and lipid A
acylation
Murein lipoprotein
Putative inner membrane protein
Scaffolding protein for murein synthesizing
machinery
Cellular processes
katEa
Catalase HPII
mgrB
Small protein involved in the acid stress response
a
bfr
Bacterioferritin
c
cspD
Cold shock-like protein
ydhJc
Undecaprenyl pyrophosphate phosphatase
maf
Septum formation protein
Central intermediary metabolism
ppac
Inorganic pyrophosphatase
a
phoA-psiF
Alkaline phosphatase
c
puuD
γ-glutamyl-γ-aminobutyrate hydrolase
Energy metabolism
treY
Malto-oligosyltrehalose synthase
puuB
γ-glutamylputrescine oxidase
e
nrfABCDEFG
Formate-dependent cytochrome c552/ nitrite
reductase
Fatty acid and phospholipid metabolism
cdh
CDP-diacylglycerol pyrophosphatase
Protein fate
tisB
LexA-regulated toxic peptide
yfiD
Autonomous glycyl radical cofactor
degPd
Periplasmic serine protease
Average
fold
change
2.18
1.91
1.87
1.78
1.77
1.75
1.73
1.69
1.59
2.79
2.39
2.06
1.77
1.72
1.54
11.53
3.42
1.58
1.88
1.68
1.54
2.04
2.68
2.52
2.46
ROD29711
clpSc
tatE
iraP
Transcription
phoBRb,c
T3SS component
ATP-dependent Clp protease adaptor protein
Twin arginine translocase protein E
Anti-adaptor protein for σs stabilisation
2.21
1.83
1.68
1.53
DNA-binding response regulator in twocomponent regulatory system with PhoR (or CreC)
rraA
Regulator of ribonuclease activity A
yfeD
Predicted DNA-binding transcriptional regulator
bssRc
Biofilm regulator
rpoS
RNA polymerase sigma factor
caiF
DNA-binding transcriptional activator of carnitine
metabolism
dksA
RNA polymerase-binding transcription factor
mlrA
DNA-binding transcriptional regulator of curli and
extracellular matrix synthesis
rcsB
DNA-binding transcriptional regulator for colanic
capsule biosynthesis
Transport and binding proteins
phoEb,c
Outer membrane phosphoporin protein E
ugpBAECQa,b,c
Glycerol-3-phosphate transport system
b,c
pstSCAB-phoU
High-affinity phosphate ABC transport system
gntX
Gluconate periplasmic binding protein
rcnA
Nickel/cobalt efflux system
yjePd
Predicted mechanosensitive channel
a,c
artJ
Arginine-binding periplasmic protein 2
c
ppx
Exopolyphosphatase
ftnA
Ferritin iron storage protein
cutC
Copper homeostasis protein
sapF
Predicted antimicrobial peptide transporter subunit
Unknown function
ygiWa
Conserved outer membrane protein
a,b
ytfK
Conserved protein
ROD23211
Small hypothetical protein
ROD05111
Hypothetical protein
ROD19811
Hypothetical protein
ROD05801
Putative small toxic membrane polypeptide
ycfP
Conserved protein
4.32
ROD09131
yjdJa
2.46
2.44
Hypothetical protein
Predicted acyltransferase with acyl-CoA Nacyltransferase domain
2.38
1.84
1.83
1.77
1.72
1.65
1.63
1.57
7.75
4.81
3.62
3.33
2.30
1.77
1.74
1.65
1.63
1.55
1.51
5.70
4.91
3.89
3.29
2.53
2.49
2.46
ROD14971
ROD36211
yodCa
ycfP
phoHb,c
ROD36481
ROD23581
ROD17581
ROD26221
yeaQ
chbG
ROD20361
ybeD
Small hypothetical protein
Hypothetical protein
Hypothetical protein
Conserved protein
Putative phosphate starvation-inducible protein
Hypothetical protein
Hypothetical protein
Hypothetical protein
Hypothetical protein
Conserved inner membrane protein
Conserved protein
Hypothetical protein
Conserved protein required for swarming
phenotype
yeeD
Conserved protein
mraZ
Conserved protein
c
yeaL
Conserved inner membrane protein
a
yodC
Hypothetical protein
yggEa
Putative immunogenic protein
Biosynthesis of cofactors, prosthetic groups, and carriers
cybB
Cytochrome b561
ynfK
Putative dethiobiotin synthetase
nudB
Dihydroneopterin triphosphate
pyrophosphohydrolase
Mobile and extrachromosomal element functions
ROD02521
Predicted tail sheath protein
ROD25761
Hypothetical prophage protein
ymfH
e14 prophage
ROD25891
Bacteriophage tail tube protein
ROD25951
Phage major capsid protein E
a
Genes belonging to the RpoS regulon
b
Genes belonging to the Pho regulon
c
Indicates the presence of a putative Pho box in the gene promoter
d
Genes belonging to the Cpx regulon
e
Genes belonging to the c-AMP receptor protein (CRP) regulon
f
Genes shown in boldface are part of an operon in which two or more genes are upregulated in the pstCA mutant.
2.38
2.35
2.27
2.27
2.23
2.22
2.11
2.09
2.08
2.06
2.05
1.91
1.90
1.56
1.56
1.56
1.56
1.53
2.55
1.66
1.56
2.97
2.70
2.30
2.23
2.12