Supplemental Table 4. Oligonucleotide sequences and modifications
Name
Illumina Standard
Adapter – Forward
Illumina Standard
Adapter – Reverse
Illumina Library PCR
Primer: LPX1.1
Illumina Library PCR
Primer: LPX2.1
Illumina Sequencing
Primer
Sequence (5’ to 3’)
AcAcTcTTTcccTAcAcGAcGcTcTTccGATcxT
pGATcGGAAGAGcTcGTATGccGTcTTcTGcTTG
Modification/ Annealing
Temp.
x: phosphorothiate linkage
c: 5mC
p: phosphate
c: 5mC
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT
TCCGATCxT
x: phosphorothiate linkage
CAAGCAGAAGACGGCATACGAGCTCTTCCGATCxT
x: phosphorothiate linkage
ACACTCTTTCCCTACACGACGCTCTTCCGATCT
Socs5 BGS – Forward
AAGGGGAAAAAATTAAAGTTTTG
57°C
Socs5 BGS – Reverse
AAAAACTTCAAAAAACACACACA
57°C
Otud7a – Intergenic
GTTGGTATATTGAGGATTGTGA
BGS – Forward
Otud7a – Intergenic
ATTCCAAAACTCAATTTCAAAC
BGS – Reverse
Standard Illumina adaptor sequences were synthesized and used as described in Gu et al. 20
55°C
55°C