Details of qPCR assay
Li Liao, et al. submitted to Molecular Ecology
Table 2. Information of qPCR assay of standard curves.
Gene
Efficiency (%) Y-intercept
R2
Ct of NTCa
narG
106.2
35.00
1.000
No Ct
napA
101.7
33.84
0.999
No Ct
nasA
102.1
34.06
1.000
No Ct
glnAsym
107.0
33.89
1.000
No Ct
gltBsym
104.0
33.53
0.999
No Ct
rpoA
109.0
35.25
0.999
36.75
cysG
105.5
33.37
1.000
No Ct
gyrB
107.6
33.18
1.000
No Ct
mraY
101.2
34.13
1.000
No Ct
glnARid
91.6
37.15
0.998
No Ct
gltBRid
103.7
34.35
1.000
35.99
ACTB
103.2
35.23
0.997
No Ct
aNo template control.
Slope Threshold
-3.182
0.8205
-3.283
0.6752
-3.272
0.9635
-3.165
0.7804
-3.229
0.6907
-3.123
0.7765
-3.197
0.9201
-3.153
0.7976
-3.293
0.7947
-3.541
0.8929
-3.237
0.8183
-3.248
0.7355