1
Table S1. The CsTFA BDs of the selected RNA fractions and the analyzed sequence number in Illumina sequence libraries
Mixing ratio of
the E. coli RNA
0.05%
0.01%
Density fraction
BDb
mixture
1H
2H
L
1.796
1.789
1.767
(±0.005)
(±0.005)
(±0.002)
rep1=32,394
rep1=31,917
rep1=39,564
rep2=26,367
rep2=23,903
rep2=32,384
rep3=31,106
rep3=31,455
rep3=34,984
rep1=5,660
rep1=1,966
rep1=163
rep2=2,611
rep2=2,147
rep2=126
rep3=3,956
rep3=257
rep3=16
Unlabeled
mixture
1H
2H
L
1.797
1.791
1.769
(±0.006)
(±0.004)
(±0.004)
rep1=31,310
rep1=56,046
rep1=40,907
rep2=65,264
rep2=86,749
rep2=191,184
rep3=35,006
rep3=39,135
rep3=62,195
rep1=505
rep1=393
rep1=169
rep2=201
rep2=201
rep2=928
rep3=1,356
rep3=75
rep3=30
1H
2H
L
1H
2H
L
1H
2H
L
1.798
1.791
1.768
1.797
1.792
1.768
1.798
1.792
1.772
(±0.004)
(±0.004)
(±0.003)
(±0.006)
(±0.006)
(±0.005)
(±0.002)
(±0.001)
(±0.003)
rep1=32,934
rep1=35,907
rep1=38,037
rep1=45,271
rep1=28,150
rep1=42,636
rep1=25,790
rep1=32,490
rep1=31,356
rep2=12,480
rep2=23,654
rep2=47,652
rep2=22,384
rep2=51,622
rep2=683,517
rep2=99,112
rep2=30,841
rep2=19,913
rep3=27,311
rep3=36,115
rep3=35,156
rep3=28,764
rep3=49,499
rep3=44,614
rep3=35,428
rep3=29,923
rep3=51,043
rep1=1,562
rep1=603
rep1=209
rep1=429
rep1=204
rep1=164
rep1=859
rep1=516
rep1=107
rep2=582
rep2=320
rep2=110
rep2=203
rep2=305
rep2=883
rep2=2,846
rep2=236
rep2=81
rep3=932
rep3=322
rep3=14
rep3=271
rep3=342
rep3=183
rep3=852
rep3=144
rep3=14
1H
2H
L
1.799
1.790
1.770
(±0.006)
(±0.006)
(±0.006)
rep1=21,465
rep1=34,642
rep1=36,667
rep2=20,610
rep2=29,624
rep2=66,080
rep3=37,767
rep3=25,523
rep3=32,490
rep1=174
rep1=323
rep1=125
rep2=98
rep2=80
rep2=710
rep3=372
rep3=121
rep3=26
mixture
1H
2H
L
1.797
1.791
1.770
(±0.004)
(±0.005)
(±0.004)
rep1=32,760
rep1=44,891
rep1=45,296
rep2=231,738
rep2=38,758
rep2=22,278
rep3=32,114
rep3=24,266
rep3=40,634
rep1=934
rep1=1,046
rep1=177
rep2=655
rep2=95
rep2=72
rep3=178
rep3=134
rep3=20
Unlabeled
mixture
1H
2H
L
1.797
1.790
1.770
(±0.005)
(±0.007)
(±0.001)
rep1=36,891
rep1=45,307
rep1=47,268
rep2=249,377
rep2=46,497
rep2=49,897
rep3=35,212
rep3=36,019
rep3=36,911
rep1=849
rep1=717
rep1=211
rep2=4,557
rep2=98
rep2=192
rep3=179
rep3=165
rep3=10
13C
13C
mixture
Unlabeled
mixture
0.001%
13C
mixture
Unlabeled
mixture
0.0001%
Number of sequences
Fractiona
Mixtures
13C
Sequences from total bacteria c
Sequences from E. coli c
2
3
a
Density fractions of RNA are indicated as “1H” (the heaviest), “2H” (second-heaviest), and “L” (light) fractions.
4
b
The CsTFA BDs (g ml−1) of the RNA fractions were determined from each set of different tubes for isopycnic centrifugation done in
1
5
triplicate. The standard deviations of three replications are indicated in parentheses.
6
c
7
and the sequence number in each replication is presented.
The 16S rRNA sequences were phylogenetically characterized from each set of the different sequence libraries constructed in triplicate,
8
9
10
2