SCIENTIFIC SERVICES KONIK HPLC APPLICATION BOOK 1 SCIENTIFIC SERVICES Contents ENVIRONMENTAL APPLICATIONS FOOD APPLICATIONS PHARMACEUTICAL APPLICATIONS CHIRAL APPLICATIONS CHIRAL APPLICATIONS 2 SCIENTIFIC SERVICES Environmental Applications 1-Determination of multiple Additives in cosmetics 2-Determination of Aldehydes and Ketones as DNPH derivates 3-Ion pair chromatography of Alkyl Sulphates and Alkyl Sulphosuccinates 4-Anion determination according to U.S. EPA Method 300.0 / 317.0 and 326.0. 5-Determination of Anions with Ion pair chromatography and indirect UV detection 6-Determination of Carbamate Insecticides by HPLC with post-column derivatization . 7-Determination of monovalent Cations . 8-Determination of divalent Cations. 9-Separation of Explosives. 10-Biomonitoring of Hydroxypyrene . 11-Determination of Ketones as DNPH Derivates . 12-Analysis of Nitro Polycyclic Aromatic Hydrocarbons. 13-Separation of ortho-, nitro- para Nitroaniline. 14-Fast Determination of PAH . 15-Fast Separation of PAHs on a 2 mm ID column. 16-Separation of Parabens . 17-Chromatographic determination of Pesticides. 18-Determination of Phenoxy acid herbicides . 19-Determination of Priority Pollutant Phenols . 20-Determination of non-ionic Surfactants using HPLC. 21-Analysis of Surfactants in toothpastes by HPLC . 22-Determination of aromatic Hydrocarbon types in middle distillates acc. to prE 3 SCIENTIFIC SERVICES 4 SCIENTIFIC SERVICES 5 SCIENTIFIC SERVICES 6 SCIENTIFIC SERVICES 7 SCIENTIFIC SERVICES 8 SCIENTIFIC SERVICES 9 SCIENTIFIC SERVICES 10 SCIENTIFIC SERVICES 11 SCIENTIFIC SERVICES 12 SCIENTIFIC SERVICES 13 SCIENTIFIC SERVICES 14 SCIENTIFIC SERVICES 15 SCIENTIFIC SERVICES 16 SCIENTIFIC SERVICES 17 SCIENTIFIC SERVICES 18 SCIENTIFIC SERVICES 19 SCIENTIFIC SERVICES 20 SCIENTIFIC SERVICES 21 SCIENTIFIC SERVICES 22 SCIENTIFIC SERVICES 23 SCIENTIFIC SERVICES 24 SCIENTIFIC SERVICES 25 SCIENTIFIC SERVICES Food Applications 1-Separation of Additives in soft drinks . 2-Determination of Aflatoxins by HPLC with post column derivatization 3-Simultaneous determination of Alcohols, Carbohydrates and Organic Acids . 4-Determination of Alcohols, Carbohydrates and Organic Acids II . 5-Separation of Amines in wine with precolumn derivatization (DANSYL) . 6-HPLC determination of Amines in wine with precolumn derivatization (OPA) . 7-Determination of Amino Acids in beer with precolumn derivatization (OPA) . 8-Inorganic and Organic Anions in beer . 9-Anthocyanins in red and rose wine . 10-Determination of Biogenic Amines in food products by HPLC in combination with ninhydrin derivatization . 11-Separation of β-Carotene isomers . 12-Determination of Diketopiperazine in soft drinks . 13-Determination of Fatty acids . 14-Analysis of Flavonoids in fruit juice . 15-Stilbene and Flavonoids in red wine samples . 16-Analysis of Flavonoid Glycosides . 17-Separation of Methylfurfural and Hydroxyfurfural in beer . 18-Determination of Mono- and Disaccharides . 19-Determination of Deoxynivalenol (DON) . 20-Determination of Nivalenol (NIV) and Deoxynivalenol (DON) with postcolumn derivatisation . 21-Analysis of Fusarium Mycotoxines . 22-Ion chromatography for determination of NO2- and NO3- in beverages . 26 SCIENTIFIC SERVICES 23-Determination of Organic Acids . 24-Determination of Organic Acids II . 25-Determination of Patulin in apple juice . 26-Separation of Preservatives . 27-Determination of various Sterols . 28-Separation of water soluble Vitamins . 29-Separation of Tocopherols and Vitamin D2 . 30-Separation of fat soluble Vitamins . 31-Fat soluble Vitamins by HPLC with electrochemical detection. 27 SCIENTIFIC SERVICES 28 SCIENTIFIC SERVICES 29 SCIENTIFIC SERVICES 30 SCIENTIFIC SERVICES 31 SCIENTIFIC SERVICES 32 SCIENTIFIC SERVICES 33 SCIENTIFIC SERVICES 34 SCIENTIFIC SERVICES 35 SCIENTIFIC SERVICES 36 SCIENTIFIC SERVICES 37 SCIENTIFIC SERVICES 38 SCIENTIFIC SERVICES 39 SCIENTIFIC SERVICES 40 SCIENTIFIC SERVICES 41 SCIENTIFIC SERVICES 42 SCIENTIFIC SERVICES 43 SCIENTIFIC SERVICES 44 SCIENTIFIC SERVICES 45 SCIENTIFIC SERVICES 46 SCIENTIFIC SERVICES 47 SCIENTIFIC SERVICES 48 SCIENTIFIC SERVICES 49 SCIENTIFIC SERVICES 50 SCIENTIFIC SERVICES 51 SCIENTIFIC SERVICES 52 SCIENTIFIC SERVICES 53 SCIENTIFIC SERVICES 54 SCIENTIFIC SERVICES 55 SCIENTIFIC SERVICES \ 56 SCIENTIFIC SERVICES 57 SCIENTIFIC SERVICES 58 SCIENTIFIC SERVICES Pharmaceutical Applications 1-Quality control mixture of Acetyl Cystein . 2-Separation and determination of Opium Alkaloids . 3-HPLC method for the determination of Amineptine and its main Metabolite in human plasma . 4-Determination of Amino Acids with OPA . 5-Separation of Aniline derivatives with electrochemical detection 64 6-Separation of Anilines and Sulfonamides by HPLC with electrochemical detection . 7-Separation of Trimethoprim and Sulfamethoxazol . 8-Determination of Anticonvulsant drugs and Methyl Xanthine derivatives in serum . 9-Separation of Antiepileptics in serum . 10-Separation of Antirheumatic drugs . 11-Separation of Catecholamines . 12-Determination of Catecholamines II . 13-Clinical analysis of Chloramphenicol . 14-Determination of Citrate and Oxalate in human urine . 15-Determination of Guaifenesin, Codein and Pseudoefedrin in cough syrup . 16-Isocratic separation of the Digoxin and Digitoxin series of Glycosides 75 Separation of Narcotics . 17-Analysis of β-Lactam Antibiotic (Moxalactam) . 18-Analysis of Bisphosphonate Pamidronate Disodium by precolumn derivatization and fluorescence detection . 19-Determination of Paracetamol and by-products . 20-Separation of Penicillin Antibiotics . 21-Separation of Peptides . 22-Fast separation of Proteins . 22-Separation of Sedative . 59 SCIENTIFIC SERVICES 23-Separation of Steroids . 24-Separation of Steroids II . 25-Separation of Sulfa drugs . 26-Separation of Sulfa drugs II . 27-Separation of Tricyclic Antidepressant drugs . 28-Separation of D,L-Tryptophan and Aromatic Acids. 60 SCIENTIFIC SERVICES 61 SCIENTIFIC SERVICES 62 SCIENTIFIC SERVICES 63 SCIENTIFIC SERVICES 64 SCIENTIFIC SERVICES 65 SCIENTIFIC SERVICES 66 SCIENTIFIC SERVICES 67 SCIENTIFIC SERVICES 68 SCIENTIFIC SERVICES 69 SCIENTIFIC SERVICES 70 SCIENTIFIC SERVICES 71 SCIENTIFIC SERVICES 72 SCIENTIFIC SERVICES 73 SCIENTIFIC SERVICES 74 SCIENTIFIC SERVICES 75 SCIENTIFIC SERVICES 76 SCIENTIFIC SERVICES 77 SCIENTIFIC SERVICES 78 SCIENTIFIC SERVICES 79 SCIENTIFIC SERVICES 80 SCIENTIFIC SERVICES 81 SCIENTIFIC SERVICES 82 SCIENTIFIC SERVICES 83 SCIENTIFIC SERVICES 84 SCIENTIFIC SERVICES 85 SCIENTIFIC SERVICES 86 SCIENTIFIC SERVICES 87 SCIENTIFIC SERVICES 88 SCIENTIFIC SERVICES 89 SCIENTIFIC SERVICES 90 SCIENTIFIC SERVICES Chiral Applications (HPLC) 1-Chiral separation of Abscisic Acid (4-Oxo-2-Cyclohexen-1-yl)-3-Methyl2,4-Pentanedienoic Acid) 2-Chiral separation of 3-Amino-2(2-Naphtyl)-1-Phenyl-Propanol . 3-Chiral separation of Atenolol (2-[4-[2-Hydroxy-3 (1-)Propoxy]Phenyl]Ethanamid). 4-Chiral separation of Atropine (rac-Hyoscyamin) . 5-Chiral separation of 1-Aza[6]Helicene . 6-Chiral separation of 1-Benzene-2-Naphthene-3-Amino Propan(1)ol . 7-Chiral separation of Benzoin (α-Hydroxy-α-Phenylacetophenon) . 8-Chiral separation of Benzyl-Mandelate (Benzyl-2-Hydroxy-3-Phenylpropionate) . 9-Chiral separation of Benzyl-Mandelate (Benzyl-2-Hydroxy-3-Phenylpropionate) 10-Chiral separation of 2,2´Bis(Diphenylphosphinoxid)3,3´Bibenzo[b]Thiophene . 11-Chiral separation of BITIANP 12- 2,2´Bis(Diphenylphosphino)3,3´Bibenzo[b]Thiophene . 13-Chiral separation of 4-Bromphenyl-Glycinamid (2-(4-Bromphenyl)-2-Amino Acetamid) . 14-Chiral separation of Carbinoxamine (2-[(4-Chlorophenyl)-Pyridin-2-yl-Methoxy]N,N-Dimethyl-Ethanamine) . 15-Chiral separation of Citalopram (1-(3-Dimethylaminopropyl)-1-(4-Fluorphenyl)3H-2-Benzofuran-5-Carbonitrile). 16-Chiral separation of Dilactid . 17-Chiral separation of 1,2-Diphenyl-2-(Tosylamino)Ethanone . 18-Chiral separation of Ethyl-Mandelate . 19-Chiral separation of Etozolin (Ethyl(3-Methyl-4-Oxo-5-Piperidinothiazolidin91 SCIENTIFIC SERVICES 2-ylidene)Acetate) . 20-Chiral separation of Flavanone (2-Phenyl-1,4-Benzopyrone) . 21-Chiral separation of Hexahydro-6,6-Dimethyl-2-Tosylpyrrolo [1,2-e]Imidazol-3-one . 22-Chiral separation of Hydroxyzine . 23-Chiral separation of Lactic Acid . 24-Chiral separation of n-Methylephedrin (2-Dimethylamino-1-Phenyl-Propanol) . 25-Chiral separation of Methyl phenyl sulfoxide . 26-Chiral separation of Methyl phenyl sulfoxide II . 27-Chiral separation of Metoprolol (1-(Isoppropylamino)-3-[4-(2Methoxyethyl)Phenoxy]Propan-2-ol) . 28-Chiral separation of 1-(Naphthalene-6-yl)Ethane-1,2-diol . 29-Chiral separation of Naproxen (6-Methoxy-α-Methyl-2-Naphthylessigsäure) . 30-Chiral separation of 1-Phenylethylenglycol (1-Phenylethane-1,2-diol) . 31-Chiral separation of 1-Phenylpropane-1,2-diol . 32-Chiral separation of Pindolol (1-(1H-Indol-4-yloxy)-Propan-2-ol) . 33-Chiral separation of Pindolol (1-(1H-Indol-4-yloxy)-3-(1- Propan-2-ol) 34-Chiral separation of Propranolol (1-Isopropylamino-3-(1-Naphthyloxy)2-Propanol) . 35-Chiral separation of Propranolol (1-Isopropylamino-3-(1-Naphthyloxy)2-Propanol) . 36-Chiral separation of Warfarin (4-Hydroxy-3-(3-Oxo-1-Phenyl-Butyl)-Cumarin) . 92 SCIENTIFIC SERVICES 93 SCIENTIFIC SERVICES 94 SCIENTIFIC SERVICES 95 SCIENTIFIC SERVICES 96 SCIENTIFIC SERVICES 97 SCIENTIFIC SERVICES 98 SCIENTIFIC SERVICES 99 SCIENTIFIC SERVICES 100 SCIENTIFIC SERVICES 101 SCIENTIFIC SERVICES 102 SCIENTIFIC SERVICES 103 SCIENTIFIC SERVICES 104 SCIENTIFIC SERVICES 105 SCIENTIFIC SERVICES 106 SCIENTIFIC SERVICES 107 SCIENTIFIC SERVICES 108 SCIENTIFIC SERVICES 109 SCIENTIFIC SERVICES 110 SCIENTIFIC SERVICES 111 SCIENTIFIC SERVICES 112 SCIENTIFIC SERVICES 113 SCIENTIFIC SERVICES 114 SCIENTIFIC SERVICES 115 SCIENTIFIC SERVICES 116 SCIENTIFIC SERVICES 117 SCIENTIFIC SERVICES 118 SCIENTIFIC SERVICES 119 SCIENTIFIC SERVICES 120 SCIENTIFIC SERVICES 121 SCIENTIFIC SERVICES 122 SCIENTIFIC SERVICES 123 SCIENTIFIC SERVICES 124 SCIENTIFIC SERVICES 125 SCIENTIFIC SERVICES 126 SCIENTIFIC SERVICES 127 SCIENTIFIC SERVICES Chiral Applications (SFC) 1-Chiral separation of Benzyl-Mandelate (Benzyl-2-Hydroxy-3-Phenylpropionate) 2-Chiral separation of 1,1´-Bi-2-Naphthol (BINOL) 3-Chiral separation of Ethyl-Mandelate . 4-Chiral separation of Hydrobenzoin (1,2-Diphenylethane-1,2-diol) . 5-Chiral separation of trans Stilbene Oxide . 6-Chiral separation of Warfarin (4-Hydroxy-3-(3-Oxo-1-Phenyl-Butyl)-Cumarin) . 128 SCIENTIFIC SERVICES 129 SCIENTIFIC SERVICES 130 SCIENTIFIC SERVICES 131 SCIENTIFIC SERVICES 132 SCIENTIFIC SERVICES 133 SCIENTIFIC SERVICES 134 SCIENTIFIC SERVICES 135