OIE Collaborating Centre Reports
Activities in 2012
Title of Collaborating Centre:
Address of Collaborating Centre:
Animal Feed Safety and Analysis
Food and Agricultural Materials Inspection Centre
(FAMIC)
2-1, Shintoshin, Chuo-ku, Saitama-shi,
Saitama 330-9731 , JAPAN
Tel.:
+81-50-3797-1859
Fax:
+81-48-600-2372
e-mail address:
website:
Name of Director of Institute
(Responsible Official):
Name (including Title and
Position) of Head of the
Collaborating Centre (formally
OIE Contact Point):
Name (including Title and
Position) of writer of this report
(if different from above)
norio_aita@nm.famic.go.jp
http://www.famic.go.jp/ffis/feed/
Dr. Masaaki YOSHIBA
Mr. Toshirou TAKEHARA
Vice-President
Mr. Norio AITA
Director, Feed Analysis II Divison
Fertilizer and Feed Inspection
Department
Annual reports of OIE Reference Centres, 2012
1
Title of Collaborating Centre
Summary of activities specifically related to the mandate of
OIE Collaborating Centres
1.
Activities as a centre of research, expertise, standardisation and dissemination of
techniques within the remit of the mandate given by the OIE
 Mr Toshirou Takehara , Dr Masami Takagi , Mr Norio Aita, Mr Toshiaki Yamata , Ms Mari Tabata and Ms
Fumika Takatsu participated in Regional Seminar for OIE National Focal Points on Animal Production Food
Safety held on October 10 to November 2 , 2012 in Tokyo, Japan, and presented reports on “Feed-borne
hazards to human and animal health” and “Activities as an OIE Collaborating Centre for Feed Safety”.
 Mr Takayuki Ishibashi , Mr Yoshihiro Sekiguchi and Mr Yoshiyasu Hashimoto participated in the 4th
International FEED SAFETY Conference “Methods and Challenges” held on September 11 to 13, 2012 in
Beijing , China, and presented reports on “Rapid Identification of Seven Serovars of Salmonella spp. In Feed
by Multiplex PCR” , “Determination of Melamine in Feeds by LC-MS/MS” and “Assessment of Morinaga
ELISA kit Ver 2. Detecting Bovine Protein in Feed Analysis”.
 Mr Norio Aita and Mr Toshiaki Yamata participated in the 2012 International Symposium Alternatives to
Antibiotics “Challenges and Solutions in Animal Production” held on September 25 to 28 ,2012 in Paris,
France.
 FAMIC developed, validated or improved the following methods of analysis for animal feed:
 Method of analysis for the determination of clothianidin, dinotefuran and thiamethoxam in rice straw,
whole-crop rice silage and paddy rice for feed by LC-MS/MS
 Method of analysis for the determination of carbaryl and 8 pesticides in rice straw, whole-crop rice and
paddy rice silage for feed by LC-MS/MS
 Method of analysis for the determination of chloramphenicol in dried skim milk by LC-MS/MS
 Method of analysis for the determination of deoxynivalenol in dry and semi-dry type pet foods by LCMS
 Method of analysis for the determination of methamidophos in dry and semi-dry type pet food by LCMS/MS
 Method of analysis for the determination of organochlorine pesticides in wet type pet food by GC-ECD
 Method of analysis for the determination of glyphosate, glufosinate and their metabolites in pet food by
LC-MS/MS
 FAMIC supplied the following information about feed safety and analysis, not only for Japan but also for
feed-related manufactures in the world.
 Results of the monitoring of feed and feed ingredients in Japan
 Hazard card for important hazards of animal health and public health importance in animal feed
 Analytical standards of feed in Japan
2.
Proposal or development of any procedure that will facilitate harmonisation of international
regulations applicable to the surveillance and control of animal diseases, food safety or
animal welfare
 FAMIC has been working on a joint project with Oregon State University for development of analytical
method of lolitrem B in grass since August, 2009. Ms Hosokai has been examining the extraction of lolitrem
B in grass since 2010. Oregon State University and FAMIC conducted the collaborative study using
common sample, in 2012.
 FAMIC has participated to a proficiency test for the determination of processed animal proteins (PAPs)
including meat and bone meal (MBM) in feed organized by the EURL-AP since 2009. Mr. Yakata
examined a proficiency test for the detection of PAP’s in feed by light microscopic methods in 2012.
3.
Networking
2
a)
Maintenance of a network with other OIE Collaborating Centres designated for the same
specialty, and
None
b)
Should the need arise, maintenance of a network with Collaborating Centres in other
disciplines
Surveillance of antimicrobial resistance bacteria－JVARM (the Japanese Veterinary Antimicrobial
Resistance Monitoring System) has been in place since 1999 in response to international concern about
the impact of antimicrobial resistance on public health. In this system, FAMIC has a vital role in
analyzing faecal samples for the presence of antimicrobial resistant bacteria in collaboration with the
Annual reports of OIE Reference Centres, 2012
Title of Collaborating Centre
NVAL (OIE Collaborating Centre for Diagnosis and Control of Animal Diseases and Related Veterinary
Product assessment in Asia) in collaboration with Livestock Hygiene Service Centers which belong to
prefectural offices in Japan.
4.
Placement of expert consultants at the disposal of the OIE
None
5.
Provision of scientific and technical training, within the remit of the mandate given by the
OIE, to personnel from OIE Member Countries
 Twelve government officials from Indonesia, Laos, Mongolia, Myanmar, Pakistan, Sudan, Uganda, Vietnam
and Zambia visited FAMIC on February 8, 2012 under the JICA Training Course on Administration of
Livestock Industry Project. They were given lectures on the following topics: animal feed safety
regulations, analysis of nutrient components of feeds, microscopic and ELISA techniques for detection of
animal protein, PCR technique for detection of animal derived DNA for prevention of BSE, and chemical
hazards in feeds.
 Seven government officials from China, Indonesia, Laos, Nicaragua, Sri Lanka and Vietnam visited the
FAMIC on June 4, 2012 under the JICA Group Training Course on Sustainable Pasture Based Livestock
Farming Development. They were given lectures on the following topics: animal feed safety regulations,
analysis of nutrient components of feeds, and microscopic and ELISA techniques for detection of animal
protein, PCR technique for detection of animal derived DNA for prevention of BSE, and determination of
radioactive contamination in feed.
 Mr Norio Aita gave a lecture on animal feed safety regulations to nine government officials from Argentina,
Bhutan, Brazil, Cambodia, Fiji, Indonesia, Kosovo, Samoan and Vietnam, on August 31, 2012 at JICA
Tokyo International Center, Shibuya, Tokyo. Their visit to Japan was part of the Japan International
Cooperation Agency’s (JICA) Food Safety Policy Making and Management Project.
 Three government officials from Taipei (Chinese) visited FAMIC on November 5, 2012. They were given
lectures on the following topics: outline of spot inspection for feeds in Japan, introduction of methods for
analyzing pathogenic microbe, animal-derived protein and animal-derived DNA and introduction of methods
for analyzing agricultural chemicals, etc.
6.
Organisation of scientific meetings on behalf of the OIE
None
7.
Coordination of scientific and technical studies in collaboration with other laboratories,
organisations or collaborating centres
Development of analysis methods for detection of processed animal protein in feed - FAMIC developed a
new ELISA kit for detection of animal (ruminant, swine and chicken) proteins in feed, in collaboration with
the Morinaga Biochemistry Laboratory.
Development of analysis methods for detection of mycotoxins in feed - With the objective of preventing
damages to livestock health arising from the use of feed contaminated with mycotoxins,FAMIC conducted a
monitoring of contamination of feed with mycotoxins and developed ELISA methods for detection of
mycotoxins, in collaboration with the National Institute of Animal Health.
Development of analysis methods for quick detection of Salmonella in feed -With the objective of
preventing damages to livestock health arising from the uses of feed contaminated with Salmonella (SE, SP,
SD and ST), FAMIC developed a quick detection method of PCR using serotype specific primers, in
collaboration with the National Institute of Animal Health.
Development of analysis methods for chemical hazard in pet-food-With the objective of preventing
damages to livestock health arising from the use of pet food contaminated with undesirable substances,
FAMIC developed the chemical analysis method for determination of melamine, histamine and pesticides, in
collaboration with the Chemical Evaluation and Research Institute.
Study of analysis methods and surveillance for mycotoxins in cereals - FAMIC developed the chemical
analysis method for determination of mycotoxins in cereals, and conducted a surveillance on them, in
collaboration with National Institute of Health Sciences.
8.
Publication and dissemination of any information within the remit of the mandate given by
the OIE that may be useful to Member Countries of the OIE
1) Hashimoto T., Hashimoto S., Matuzaki M., Sekiguchi Y., Hashimoto Y., Asao M.,& Takagi M. (2012) Investigation of Whether CLSI Broth Microdilution Method is Applicable for MICs Determination of
Enterococcus species. Foof Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi), 53, 225-232.
2) Yakata M., Kuramitsu R., Kai S., Kudo N. Yamoto R. and Matsuo S. (2012) - Development and
performance evaluation of a GC-FPD method for quantification of ethephon in feeds. Food Hygiene and
Safety Science (Shokuhin Eiseigaku Zasshi), 53(1), 45-51.
Annual reports of OIE Reference Centres, 2012
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Hiraoka H., Fukunaka R., Ishikuro E., Enishi O. and Goto T. (2012) - Improvement and validation of
method to determine neutral detergent fiber in feed. Animal Science Journal, doi: 10.1111/j.17400929.2012.01019.x.
Aoyama K., Akashi H., Mochizuki N., Ito Y., Miyashita T., Lee S., Ogiso M., Maeda M., Kai S., Tanaka
H., Noriduki H., Hiraoka H., Tanaka T., Ishikuro E., Itoh Y., Nagayama T., Nakajima M., Naito S. and
Sugita-Konisihi Y., (2012) - Interlaboratory study of LC-UV and LC-MS methods for the simultaneous
determination of deoxynivalenol and nivalenol in wheat. Food Hygiene and Safety Science (Shokuhin
Eiseigaku Zasshi), 53(3), 152-156.
Sugimoto Y. (2012) - Simultaneous determination of clothianidin, dinotefuran and thiamethoxam in rice
straw, whole-crop rice silage and paddy rice for feed by LC-MS/MS simultaneous. Research Report of
Animal Feed No.37. Food and Agricultural Materials Inspection Center, Saitama, 1-18.
Sakakibara Y. (2012) - Simultaneous determination of carbaryl and 8 pesticides in rice straw, whole-crop
silage and paddy rice for feed by LC-MS/MS. Research Report of Animal Feed No. 37. Food and
Agricultural Materials Inspection Center, Saitama, 19-45.
Yamamoto K. and Oshima S. (2012) - Determination of chloramphenicol in dried skim milk by LCMS/MS. Research Report of Animal Feed No. 37. Food and Agricultural Materials Inspection Center,
Saitama, 46-58.
Uenoyama T., Tanaka S., Yamata T. and Takahashi Y. (2012) - Determination of deoxynivalenol in dry and
semi-dry type pet food by LC-MS. Research Report of Animal Feed No. 37. Food and Agricultural
Materials Inspection Center, Saitama, 59-72.
Komoriya T. (2012) - Determination of methamidophos in dry and semi-dry type pet food by LC-MS/MS.
Research Report of Animal Feed No. 37. Food and Agricultural Materials Inspection Center, Saitama, 7383.
Yakata M. (2012) - Simultaneous determination of organochlorine pesticides in wet type pet food by GCECD. Research Report of Animal Feed No. 37 Food and Agricultural Materials Inspection Center, Saitama,
84-114.
Yamata T. and Yoshimura S. (2012) - Simultaneous determination of glyphosate, glufosinate and their
metabolites in pet food by LC-MS/MS. Research Report of Animal Feed No. 37. Food and Agricultural
Materials Inspection Center, Saitama, 115-141.
Oshima S. (2012) –Collaborative study of melamine in feeds by LC-MS/MS. Research Report of Animal
Feed No. 37. Food and Agricultural Materials Inspection Center, Saitama, 142-146.
Ishibashi T. and Tabata M. (2012) - Nested experiment for intermediate precision assessment of
determination of moisture content in wet type pet foods. Research Report of Animal Feed No. 37. Food and
Agricultural Materials Inspection Center, Saitama, 147-150.
Oshima S., Saiki M., Satou K., Takeda Z. and Tanaka S. (2012) - Proficiency test (in the fiscal 2011).
Research Report of Animal Feed No. 37. Food and Agricultural Materials Inspection Center, Saitama, 151181.
Komoriya T., Ishibashi T., Hashimoto S., Yoshinaga S., Yamata T., Aita N. and Chihara T. (2012) Monitoring result of undesirable substances in feeds (in the fiscal 2011). Research Report of Animal Feed
No. 37. Food and Agricultural Materials Inspection Center, Saitama, 182-204.
Asao M., Sasaki T., Hashimoto S., Yoshinaga S. Yamata T. and Aita N. (2012) - National assay results of
specified feed additives (in the fiscal 2011). Research Report of Animal Feed No. 37. Food and Agricultural
Materials Inspection Center, Saitama, 205-218.
Aoyama K., (2012) - Mycotoxin of food, medical and shelter No.12 Analysis methods of ochratoxin,
fumonisin and zearalenone in foods. Bokin Bobai, 40(12), 769-777.
Presentation (Oral and poster presentation)
1) Ishibashi T. et al. (2012) - Determination of Melamine in Feeds by LC-MS/MS. The 4th International FEED
SAFETY Conference “Methods and Challenges”, P04.
2) Sekiguchi Y. et al. (2012) - Rapid Identification of Seven Serovars of Salmonella spp. In Feed by Multiplex
PCR. The 4th International FEED SAFETY Conference “Methods and Challenges”, P05.
3) Hashimoto Y. et al. (2012) - Assessment of Morinaga ELISA kit Ver 2. Detecting Bovine Protein in Feed
Analysis. The 4th International FEED SAFETY Conference “Methods and Challenges”, P06.
4) Yoshimura T et al. - Development of analysis methods for determination of deoxynivalnol in pet food (dry
and semi-dry type) by LC-MS/MS. The open study/investigation meeting of FAMIC in 2012. 13-14. (in
Japanese)
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Annual reports of OIE Reference Centres, 2012