SYLLABUS
COURSE TITLE
FACULTY/INSTITUTE
COURSE CODE
DEGREE PROGRAMME
FIELD OF STUDY
PLANT IN VITRO CULTURES
BIOLOGY AND AGRICULTURE
DEGREE LEVEL
IST
BIOLOGY
COURSE FORMAT
YEAR AND SEMESTER
NAME OF THE TEACHER
COURSE OBJECTIVES
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
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STUDY MODE
FULL TIME
LABORATORY CLASSES; SEMINARS AND
INDIVIDUAL MEETINGS
WINTER
MARZENA MAZUREK
provide basic knowledge about the types of plant in vitro cultures, their breeding and
use in practice (for example: micropropagation, breeding, germplasm conservation,
production of secondary metabolites)
broaden the knowledge associated with the control of plant growth and development in
in vitro cultures
prepare students to conduct research with application of plant in vitro cultures
PREREQUISITES
Good communication, reading and writing English;
High school plant physiology level
KNOWLEDGE:
LEARNING OUTCOMES
Students will be able tocharacterize the major types of
cultures in vitro and the possibility of their use in practice.
Students will understand the influence of physical
conditions, explant types, medium ingredients on the
growth and development of plant in vitro
SKILLS:
Students will be able to plan, establish and carry out
experiments with application of in vitro plant cultures.
Students will be able to prepare culture medium and
establish in vitro plant cultures from primary and secondary
explants.
Students will be able to carry out the observations and
biometric and physiological measurements with application
of the basic techniques used in the laboratory.
SOCIAL COMPETENCES:
Students will be able to develop skills in performing
collaborative research.
COURSE ORGANISATION –LEARNING FORMAT AND NUMBER OF HOURS
LABORATORY CLASSES 25 hrs
SEMINARS; 15 hrs
INDIVIDUAL MEETINGS 5 hrs
COURSE DESCRIPTION
This course has been designed to introduce the students to the issues of in vitro cultures of
plant.
In particular we will consider the following topics:
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Explants and quality of different type of explants. Preparing explants to create in
vitro culture of plants.
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Preparing basal solutions and medium for in vitro culture. Composition of the
medium and its impact on the growth and development of plants culture.
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Work rules in Laboratory of Plant in Vitro Cultures. The rules of using lab equipment
and work in sterile conditions.
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Establishment new in vitro cultures of plant in sterile conditions.
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Passaging the cultures.
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Creation of artificial seeds.
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Evaluation of Plant Growth Regulators and theirimpact on the growth and
development ofplant in vitro cultures.
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Determination of tolerance of cultures to abiotic stresses.
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Estimation the growth and quality of cultures, microplants established.
METHODS OF INSTRUCTION
REQUIREMENTS AND ASSESSMENTS
GRADING SYSTEM
TOTAL STUDENT WORKLOAD
Practical laboratory work, Audio/video presentations,
Discussion, Reporting and presenting results
Attendance is expected in all laboratory classes,
seminars and individual meetings. Assessment for this
course is carried out in many different ways. It takes
into consideration both conduct laboratory
experiments and presentation of results but also
knowledge of the seminars.
Technical skills
10%
Reporting and presenting 40%
Final exam 50%
Grading scale:
Grade 5, > 94% ;
Grade 4.5, 90-94%;
Grade 4, 80-89%;
Grade 3.5, 70-79%;
Grade 3, 60-69% correct answers
LABORATORY CLASSES 25 hrs
NEEDED TO ACHIEVE EXPECTED
LEARNING OUTCOMES EXPRESSED
IN TIME AND ECTS CREDIT POINTS
LANGUAGE OF INSTRUCTION
INTERNSHIP
MATERIALS
SEMINARS; 15 hrs
INDIVIDUAL MEETINGS 5 hrs
READING and HOMEWORK: 40 hrs
IN TOTAL:
85 hrs 4 ECTS
English
n.a.
PRIMARY OR REQUIRED BOOKS/READINGS:
 Plant Propagation byTissue Culture3rd Edition.
2008. Volume 1. The Background . Springer
 Hussain A, Qarshi IA, Nazir H and Ullah I. 2012.
Plant Tissue Culture: Current Status and
Opportunities. Recent Advances in Plant in vitro
Culture.InTech. Chapter 1
 Saad A I M and Elshahed AM. 2012. Plant Tissue
Culture Media. Recent Advances in Plant in vitro
Culture.InTech. Chapter 2
SUPPLEMENTAL OR OPTIONAL BOOKS/READINGS:
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RemottiP. C. 1998. Somaclonal variation and in
vitro selection for crop improvement. Current
Plant Science and Biotechnology in Agriculture,
32: 169- 202
Ahloowali B.S.1998. In vitro techniques and
mutagenesis for the improvement ofvegetatively
propagated plants. Current Plant Science and
Biotechnology in Agriculture, 32: 293-310
Sarasan V*, Cripps R, Ramsay M M., Atherton C,
Mcmichen M, Prendergast G, AND Rowntree J
K.2006. Conservation in vitro of threatened plants
– progress in the past decade. In Vitro Cell. Dev.
Biol.Plant 42:206–214