ANALYSIS OF SOLVATION NUMBER,ADIABATIC
COMPRESSIBILITY
AND SPECIFIC ACOUSTIC IMPEDANCE OF
GLYCYL AND AMIDE SALTS
Padmavathy.R1,JasmineVasanthaRani.E1,Santhakumari.S2,Sujatha.S2
1
Department of Physics,Seethalakshmi Ramaswamy College,Tiruchirappalli,Tamilnadu
2
Department of Physics,Srimathi Indira Gandhi College,Tiruchirappalli,Tamilnadu
ABSTRACT
Ultrasound is novel and challenging method that is being developed for biomolecules
where non-invasive technique is of great interest. Ultrasonic Velocity measurements are
highly sensitive to molecular interactions and used to provide qualitative information about
the physical nature and strength of the moleculer/ inter-ionic interactions.The aminoacids
belong to an important family of biomolecules which serve primarily as the basic building
block of all proteins.The Sample glycyl L-leucine is used in the study of protein structure
and fuctions .Another Sample sulphanilamide is the potent antibiotic which is important in
the control of urinary tract infections and mening gococcal meningitis profilaxes. Solvation
is the association of solvent molecules with the solute ions in a solutions .Adiabatic
compressibility of a solution varies due to the effect of hydrogen bonding or induced dipoledipole interactions. Specific acoustic impedance is the characteristic impedance of the
medium.In the present investigation ,the solutions of different molalities were prepared with
the samples in non-aqueous medium and experiments were carried out from a low
concentration to high concentration,at different temperature.The measured values of
density,viscosity and ultrasonicvelocity were used to compute solvation number,adiabatic
compressibility and Specific acoustic impedance.An attempt is made to identify the entry
solvate into the Samples and the entry of biological molecules into the Solvate Which
supports to identify the molecular structure.
KEYWORDS- ion-solvent and solute-solvent interactios.
1
INTRODUCTION
Amino acids belong to an important family of biomolecules which serve primarily as the
basic building block of all proteins[1].. Ultrasonic studies of solutions yield
valuableinformation about the molecular interactions, the nature and strength of the
interactions.Sulphanilamide is the parent compound of all the sulpha-durgs which is
important in urinarytract infections and meningococcal meningitis profilaxes. In the present
work, non-aqueoussolutions of Sulphanilamide and glycyl-L-Leucine have been prepared
with differentconcentrations and the experiments were carried out from a low temperature of
50c to a hightemperature of 550c. The ultrasonic velocity data combined with density and
viscosityprovides the standard means for determining the Solvation number and acoustical
parameters.(adiabaticcompressibility,specific acoustic impedance).
EXPERIMENTAL TECHNIQUE
Density of the solutions is measured using 25ml specific gravity bottle with accuracy
of 0.0001gm/cc. Cannon Fenske viscometer is used for the viscosity measurements, with an
accuracy of ± 0.5%. Mittal’s interferometer of frequency 2 MHZ with an accuracy ±0.5m/s is
used for the measurement of ultrasonic velocity.
COMPUTATION
The Solvation number and acoustic parameters are computed by using the formulae.
(i)Solvation number (Sn) = n1/n2 [1-β/βo]
(ii) Adiabatic compressibility (β) = [1/u2ρ] cm2/dyne
(iii)Specific acoustic impedance Z =(ρu)Rayl.
2
TABLES AND FIGURES
TABLE-(1)a
Solvation number- Glycyl-L-Leucine
Molality(m)
50c
150c
250c
350c
450c
550c
0.001
58.82
314.75
343.16
557.71
962.71
1333.57
0.005
43.45
84.78
91.44
145.46
231.97
306.52
0.01
35.69
57.41
75.15
110.24
156.50
193.58
0.015
40.41
53.62
55.44
82.78
115.69
135.45
0.02
38.17
51.79
54.09
68.86
96.37
112.05
SULPHANILAMIDE-TABLE-(1)b
Molality(m)
5°c
15°c
25°c
35°c
45°c
55°c
0.001
124.50
357.99
15.97
285.67
492.64
778.43
0.005
186.96
220.10
270.72
327.37
344.78
480.80
0.01
56.74
59.19
72.86
103.49
93.32
120.57
0.015
49.59
52.41
57.42
79.72
77.21
94.55
0.02
40.21
45.50
51.85
68.64
76.46
80.88
3
FIG(1)a
Glycyl-L-Leucine
Solvation number Vs Molality
1400
1200
5°c
Solvation number
1000
15°c
800
25°c
35°c
600
45°c
400
55°c
200
0
.001
.005
.01
0.015
0.02
Molality(m)
SULPHANILAMIDE –FIG(1)b
Solvation number Vs Molality
800
Solvation number
700
5°c
600
15°c
500
25°c
400
35°c
45°c
300
55°c
200
100
0
.001
.005
.01
.015
.02
Molality(m)
4
Adiabatic compressibility(cm2/dynes)x10-11
Glycyl-L-Leucin- TABLE(2)a
Molality(m)
Temperature in centigrade
5
15
25
35
45
55
0.001
3.239
3.292
3.359
3.470
3.542
3.590
0.005
3.215
3.275
3.341
3.442
3.507
3.554
0.01
3.194
3.251
3.294
3.379
3.437
3.482
0.015
3.156
3.215
3.281
3.356
3.407
3.465
0.02
3.132
3.179
3.241
3.333
3.374
3.427
SULPHANILAMIDE-TABLE(2)b
Molality(m)
5°c
15°c
25°c
35°c
45°c
55°c
0.001
3.23
3.28
3.41
3.52
3.62
3.69
0.005
3.19
3.27
3.35
3.45
3.59
3.66
0.01
3.16
3.24
3.32
3.39
3.55
3.61
0.015
3.14
3.22
3.28
3.36
3.51
3.58
0.02
3.13
3.20
3.28
3.33
3.44
3.54
5
Glycyl-L-LeucineFIG(2)a
Adiabatic compressibility(cm2/dynes)x10-11
Adiabatic compressibility vs Molality
3.60
3.55
3.50
3.45
3.40
3.35
3.30
3.25
3.20
3.15
3.10
5°c
15°c
25°c
35°c
45°c
55°c
.001
.005 .01 .015
Molality(m)
.02
SULFANILAMIDE-FIG(2)b
Adiabatic compressibility(cm2/dynes) 10-11
Adiabatic compressibility Vs Molality
3.8
3.7
3.6
5°c
15°c
25°c
35°c
45°c
55°c
3.5
3.4
3.3
3.2
3.1
3.0
.001
.005
.01
.015
.02
Molality(m)
SULFAN ILAMID E
6
Specific acoustic impedance (Rayl)
Glycyl-L-LeucineMolality(m)
TABLE(3)a
Temperature in centigrade
5
15
25
35
45
55
0.001
18.83
18.65
18.38
18.00
17.77
17.59
0.005
18.91
18.67
18.43
18.07
17.86
17.67
0.01
18.97
18.73
18.57
18.25
18.04
17.87
0.015
19.08
18.86
18.60
18.31
18.12
17.90
0.02
19.14
18.95
18.71
18.38
18.21
18.01
SULPHANILAMIDE-TABLE(3)b
Molality(m)
5°c
15°c
25°c *
35°c
45°c
55°c
0.001
18.85
18.65
18.22
17.87
17.55
17.34
0.005
18.96
18.66
18.45
18.02
17.63
17.40
0.01
19.05
18.74
18.54
18.21
17.74
17.53
0.015
19.13
18.85
18.60
18.27
17.84
17.61
0.02
19.15
18.90
18.68
18.36
18.01
17.71
7
Glycyl-L-LeucineFIG(3)a
SPecific accoustic impedance(Rayl)
Specific accoustic impedance Vs Molality
5°c
15°c
25°c
35°c
45°c
55°c
19.25
18.75
18.25
17.75
17.25
.001 .005
.01
.015
.02
Molality(m)
SULPHANILAMIDE-FIG(3)b
Specific accoustic impedance(rayl)
Specific accoustic impedance Vs Molality
19.5
5°c
19.0
15°c
18.5
25°c
35°c
18.0
45°c
17.5
55°c
17.0
0.001
0.005 0.01 0.015
Molality(m)
0.02
8
Results and Discussion
SOLVATION NUMBER The solvation numbers reflect the dynamic situation of the ion as its moves round the
solution[2]
comparing the two salts as shown in the table(1)a&b)and fig(1)a&b), the
solvation number is a positive.It indicates that the compressibility of the solution is less than
the solvent.The salvation approach is used to interpret ion-solvent interaction[3]. The ionsolvent interaction may be due to equal to intermolecular interaction[4].
ADIABATIC COMPRESSIBILITY -The structural changes of molecules in the solution
takes place due to the existence of electrostatic field between the interacting
molecules[5]..The arrangement of the molecules results in the effect of adiabatic
compressibility.compare the both salts, the adiabatic compressibility increases with decrease
in molality and rise in temperature [6] shown in table(2)a&b and fig (2)a&b. When the salt
is added to the solvent ,the compressibility is lowered. This lowering is attributed to the
influence of the electrostatic field of the ions on the surrounding solvent molecules. such a
decrease may be due to (i) an increase in the number of incompressible molecule[7,8,9].
(ii) structural changes occurring in the solution .This may be due to the association taking
place between the molecules. When the temperature increases, the associated groups of
molecules breakdown increasingly and the forces of attraction between the molecules
decrease. This leads to an increase in the adiabatic compressibility of the system[10].
SPECIFIC ACOUSTIC IMPEDANCE-The specific acoustic impedance is governed by
the internal and elastic properties of the medium. In the present investigation, Z increases
with increasing concentration and decreases with increasing temperature, which indicates
the absence of complex formation and the existence of solute-solvent interaction. The results
are tabulated in the table(3)a&b and shown in the fig (3)a&b for both the salts.
This linear increase due to the effect of strong solute-solvent interaction{11] existing in the
solutions. The specific acoustic impedance is available as 18.39Rayl at 250c*of formamide
from the literature[12]. In the present investigation ,the inter-molecular free length of
solutions is found to decrease with respect to the various molality and increases with
temperature. These changes reveal the fact that there is a strong interaction exist between the
solute and solvent[13]. The comparision of this three parameters gave the good agreement
results for molecular interactions.
9
CONCLUSION
The field of protein folding and stability has been a critically important area of research for
years and remains today as one of the greatest unsolved mysteries. In the present work, a
detailed analysis of acoustic and thermodynamic properties was made. The study reveals the
fact that the addition solute in the solvent enhances the solute-solvent interaction.This
behavior of glycy1-L-Leucine in formamide emphasizes that it act as a structure maker due
to its structural stability and Zwitterionic nature. Sulphanilamide compounds identified as
chemotherapeutic agents, possess broad spectrum of biological properties[14].
Sulfanilamide is a potent antibiotic because this drug undergoes metabolic alterations in
tissues[15] .The detailed study of acoustic parameters suggests that there is a molecular
association existing between the molecules of the solution. The addition of solute into the
solvent brings about a strong solute-solvent interaction in the solution
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